Xanthosine utilization in Salmonella enterica serovar Typhimurium is recovered by a single aspartate-to-glycine substitution in xanthosine phosphorylase.

Michael Riis Hansen; Jesper Tranekjær Jørgensen; Gert Dandanell

doi:10.1128/JB.01926-05

Xanthosine utilization in Salmonella enterica serovar Typhimurium is recovered by a single aspartate-to-glycine substitution in xanthosine phosphorylase.

Michael Riis Hansen, Jesper Tranekjær Jørgensen, Gert Dandanell

3 Citations (Scopus)

Abstract

xapABR from Salmonella enterica was analyzed and compared withthe corresponding Escherichia coli genes. xapB and xapR, butnot xapA, encode functional proteins. An S. enterica XapA(Asp72Gly)mutant that restores the phosphorolytic activity was selected.The purified mutant enzyme has different kinetic constants thanthe E. coli enzyme but similar substrate specificity.

Original language	English
Journal	Journal of Bacteriology
Volume	188
Issue number	11
Pages (from-to)	4153-7
ISSN	0021-9193
DOIs	https://doi.org/10.1128/JB.01926-05
Publication status	Published - 2006

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Xanthosine utilization in Salmonella enterica serovar Typhimurium is recovered by a single aspartate-to-glycine substitution in xanthosine phosphorylase. / Hansen, Michael Riis; Jørgensen, Jesper Tranekjær ; Dandanell, Gert.
In: Journal of Bacteriology, Vol. 188, No. 11, 2006, p. 4153-7.

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abstract = "xapABR from Salmonella enterica was analyzed and compared with the corresponding Escherichia coli genes. xapB and xapR, but not xapA, encode functional proteins. An S. enterica XapA(Asp72Gly) mutant that restores the phosphorolytic activity was selected. The purified mutant enzyme has different kinetic constants than the E. coli enzyme but similar substrate specificity. ",

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AU - Jørgensen, Jesper Tranekjær

AU - Dandanell, Gert

PY - 2006

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N2 - xapABR from Salmonella enterica was analyzed and compared with the corresponding Escherichia coli genes. xapB and xapR, but not xapA, encode functional proteins. An S. enterica XapA(Asp72Gly) mutant that restores the phosphorolytic activity was selected. The purified mutant enzyme has different kinetic constants than the E. coli enzyme but similar substrate specificity.

AB - xapABR from Salmonella enterica was analyzed and compared with the corresponding Escherichia coli genes. xapB and xapR, but not xapA, encode functional proteins. An S. enterica XapA(Asp72Gly) mutant that restores the phosphorolytic activity was selected. The purified mutant enzyme has different kinetic constants than the E. coli enzyme but similar substrate specificity.

U2 - 10.1128/JB.01926-05

DO - 10.1128/JB.01926-05

M3 - Journal article

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SN - 0021-9193

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JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 11

ER -

Xanthosine utilization in Salmonella enterica serovar Typhimurium is recovered by a single aspartate-to-glycine substitution in xanthosine phosphorylase.

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