Wound healing and expression of antimicrobial peptides/polypeptides in human keratinocytes, a consequence of common growth factors.

TY - JOUR

T1 - Wound healing and expression of antimicrobial peptides/polypeptides in human keratinocytes, a consequence of common growth factors.

AU - Sørensen, Ole E

AU - Cowland, Jack B

AU - Theilgaard-Mönch, Kim

AU - Liu, Lide

AU - Ganz, Tomas

AU - Borregaard, Niels

N1 - Keywords: Acute-Phase Proteins; Anti-Infective Agents; Antimicrobial Cationic Peptides; Cell Division; Cells, Cultured; Cytokines; Fibroblast Growth Factor 2; Growth Substances; Humans; Inflammation Mediators; Insulin-Like Growth Factor I; Keratinocytes; Lipocalins; Oncogene Proteins; Organ Culture Techniques; Protein Biosynthesis; Proteinase Inhibitory Proteins, Secretory; Proteins; Proto-Oncogene Proteins; RNA, Messenger; Transforming Growth Factor alpha; Transforming Growth Factor beta; Transforming Growth Factor beta1; Wound Healing; beta-Defensins

PY - 2003

Y1 - 2003

N2 - In addition to acting as a physical barrier against microorganisms, the skin produces antimicrobial peptides and proteins. After wounding, growth factors are produced to stimulate the regeneration of tissue. The growth factor response ceases after regeneration of the tissue, when the physical barrier protecting against microbial infections is re-established. We found that the growth factors important in wound healing, insulin-like growth factor I and TGF-alpha, induce the expression of the antimicrobial peptides/polypeptides human cationic antimicrobial protein hCAP-18/LL-37, human beta-defensin 3, neutrophil gelatinase-associated lipocalin, and secretory leukocyte protease inhibitor in human keratinocytes. Both an individual and a synergistic effect of these growth factors were observed. These findings offer an explanation for the expression of these peptides/polypeptides in the skin disease psoriasis and in wound healing and define a host defense role for growth factors in wound healing.

AB - In addition to acting as a physical barrier against microorganisms, the skin produces antimicrobial peptides and proteins. After wounding, growth factors are produced to stimulate the regeneration of tissue. The growth factor response ceases after regeneration of the tissue, when the physical barrier protecting against microbial infections is re-established. We found that the growth factors important in wound healing, insulin-like growth factor I and TGF-alpha, induce the expression of the antimicrobial peptides/polypeptides human cationic antimicrobial protein hCAP-18/LL-37, human beta-defensin 3, neutrophil gelatinase-associated lipocalin, and secretory leukocyte protease inhibitor in human keratinocytes. Both an individual and a synergistic effect of these growth factors were observed. These findings offer an explanation for the expression of these peptides/polypeptides in the skin disease psoriasis and in wound healing and define a host defense role for growth factors in wound healing.

M3 - Journal article

C2 - 12759437

SN - 0022-1767

VL - 170

SP - 5583

EP - 5589

JO - Journal of Immunology

JF - Journal of Immunology

IS - 11

ER -