Within-host competition between two entomopathogenic fungi and a granulovirus in Diatraea saccharalis (Lepidoptera: Crambidae)

Giuliano Pauli; Gabriel Moura Mascarin; Jørgen Eilenberg; Italo Delalibera Júnior

doi:10.3390/insects9020064

Within-host competition between two entomopathogenic fungi and a granulovirus in Diatraea saccharalis (Lepidoptera: Crambidae)

Giuliano Pauli, Gabriel Moura Mascarin, Jørgen Eilenberg, Italo Delalibera Júnior^*

^*Corresponding author for this work

Section for Organismal Biology

6 Citations (Scopus)

47 Downloads (Pure)

Abstract

We provide insights into how the interactions of two entomopathogenic fungi and a virus play a role in virulence, disease development, and pathogen reproduction for an economically important insect crop pest, the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae). In our model system, we highlight the antagonistic effects of the co-inoculation of Beauveria bassiana and granulovirus (DisaGV) on virulence, compared to their single counterparts. By contrast, combinations of Metarhizium anisopliae and B. bassiana, or M. anisopliae and DisaGV, have resulted in additive effects against the insect. Intriguingly, most cadavers that were derived from dual or triple infections, produced signs/symptoms of only one species after the death of the infected host. In the combination of fungi and DisaGV, there was a trend where a higher proportion of viral infection bearing conspicuous symptoms occurred, except when the larvae were inoculated with M. anisopliae and DisaGV at the two highest inoculum rates. Co-infections with B. bassiana and M. anisopliae did not affect pathogen reproduction, since the sporulation from co-inoculated larvae did not differ from their single counterparts.

Original language	English
Article number	64
Journal	Insects
Volume	9
Issue number	2
Number of pages	11
ISSN	2075-4450
DOIs	https://doi.org/10.3390/insects9020064
Publication status	Published - 2018

Keywords

Beauveria bassiana
Beta-baculovirus
Metarhizium anisopliae
Mixed infection
Sugarcane borer
Virulence

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10.3390/insects9020064

Within-Host Competition between Two Entomopathogenic Fungi and a Granulovirus in Diatraea saccharalis (Lepidoptera: Crambidae)Final published version, 487 KB

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title = "Within-host competition between two entomopathogenic fungi and a granulovirus in Diatraea saccharalis (Lepidoptera: Crambidae)",

abstract = "We provide insights into how the interactions of two entomopathogenic fungi and a virus play a role in virulence, disease development, and pathogen reproduction for an economically important insect crop pest, the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae). In our model system, we highlight the antagonistic effects of the co-inoculation of Beauveria bassiana and granulovirus (DisaGV) on virulence, compared to their single counterparts. By contrast, combinations of Metarhizium anisopliae and B. bassiana, or M. anisopliae and DisaGV, have resulted in additive effects against the insect. Intriguingly, most cadavers that were derived from dual or triple infections, produced signs/symptoms of only one species after the death of the infected host. In the combination of fungi and DisaGV, there was a trend where a higher proportion of viral infection bearing conspicuous symptoms occurred, except when the larvae were inoculated with M. anisopliae and DisaGV at the two highest inoculum rates. Co-infections with B. bassiana and M. anisopliae did not affect pathogen reproduction, since the sporulation from co-inoculated larvae did not differ from their single counterparts.",

keywords = "Beauveria bassiana, Beta-baculovirus, Metarhizium anisopliae, Mixed infection, Sugarcane borer, Virulence",

author = "Giuliano Pauli and Mascarin, {Gabriel Moura} and J{\o}rgen Eilenberg and {Delalibera J{\'u}nior}, Italo",

year = "2018",

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T1 - Within-host competition between two entomopathogenic fungi and a granulovirus in Diatraea saccharalis (Lepidoptera: Crambidae)

AU - Pauli, Giuliano

AU - Mascarin, Gabriel Moura

AU - Eilenberg, Jørgen

AU - Delalibera Júnior, Italo

PY - 2018

Y1 - 2018

N2 - We provide insights into how the interactions of two entomopathogenic fungi and a virus play a role in virulence, disease development, and pathogen reproduction for an economically important insect crop pest, the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae). In our model system, we highlight the antagonistic effects of the co-inoculation of Beauveria bassiana and granulovirus (DisaGV) on virulence, compared to their single counterparts. By contrast, combinations of Metarhizium anisopliae and B. bassiana, or M. anisopliae and DisaGV, have resulted in additive effects against the insect. Intriguingly, most cadavers that were derived from dual or triple infections, produced signs/symptoms of only one species after the death of the infected host. In the combination of fungi and DisaGV, there was a trend where a higher proportion of viral infection bearing conspicuous symptoms occurred, except when the larvae were inoculated with M. anisopliae and DisaGV at the two highest inoculum rates. Co-infections with B. bassiana and M. anisopliae did not affect pathogen reproduction, since the sporulation from co-inoculated larvae did not differ from their single counterparts.

AB - We provide insights into how the interactions of two entomopathogenic fungi and a virus play a role in virulence, disease development, and pathogen reproduction for an economically important insect crop pest, the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae). In our model system, we highlight the antagonistic effects of the co-inoculation of Beauveria bassiana and granulovirus (DisaGV) on virulence, compared to their single counterparts. By contrast, combinations of Metarhizium anisopliae and B. bassiana, or M. anisopliae and DisaGV, have resulted in additive effects against the insect. Intriguingly, most cadavers that were derived from dual or triple infections, produced signs/symptoms of only one species after the death of the infected host. In the combination of fungi and DisaGV, there was a trend where a higher proportion of viral infection bearing conspicuous symptoms occurred, except when the larvae were inoculated with M. anisopliae and DisaGV at the two highest inoculum rates. Co-infections with B. bassiana and M. anisopliae did not affect pathogen reproduction, since the sporulation from co-inoculated larvae did not differ from their single counterparts.

KW - Beauveria bassiana

KW - Beta-baculovirus

KW - Metarhizium anisopliae

KW - Mixed infection

KW - Sugarcane borer

KW - Virulence

U2 - 10.3390/insects9020064

DO - 10.3390/insects9020064

M3 - Journal article

C2 - 29899228

AN - SCOPUS:85049101718

SN - 2075-4450

VL - 9

JO - Insects

JF - Insects

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M1 - 64

ER -

Within-host competition between two entomopathogenic fungi and a granulovirus in Diatraea saccharalis (Lepidoptera: Crambidae)

Abstract

Keywords

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