Vitamin D is positively associated with sperm motility and increases intracellular calcium in human spermatozoa

Martin Blomberg Jensen; Poul J Bjerrum; Torben E Jessen; John E Nielsen; Ulla N Joensen; Inge A Olesen; Jørgen H Petersen; Anders Juul; Steen Dissing; Niels Jørgensen

doi:10.1093/humrep/der059

Vitamin D is positively associated with sperm motility and increases intracellular calcium in human spermatozoa

Martin Blomberg Jensen, Poul J Bjerrum, Torben E Jessen, John E Nielsen, Ulla N Joensen, Inge A Olesen, Jørgen H Petersen, Anders Juul, Steen Dissing, Niels Jørgensen

135 Citations (Scopus)

Abstract

BACKGROUND The vitamin D receptor (VDR) is expressed in human spermatozoa, and VDR-knockout mice and vitamin D (VD) deficiency in rodents results in impaired fertility, low sperm counts and a low number of motile spermatozoa. We investigated the role of activated VD (1,25(OH)(2)D(3)) in human spermatozoa and whether VD serum levels are associated with semen quality. METHODS Cross-sectional association study of semen quality and VD serum level in 300 men from the general population, and in vitro studies on spermatozoa from 40 men to investigate the effects of VD on intracellular calcium, sperm motility and acrosome reaction. All men delivered samples for routine semen analysis and blood for measurements of follicle stimulating hormone, Inhibin B, 25-hydroxy-VD, albumin, alkaline phosphatase, calcium and parathyroid hormone (PTH). RESULTS In the association study, 44% were VD insufficient (75 nM). 1,25(OH)(2)D(3) increased intracellular calcium concentration in human spermatozoa through VDR-mediated calcium release from an intracellular calcium storage, increased sperm motility and induced the acrosome reaction in vitro. CONCLUSIONS 1,25(OH)(2)D(3) increased intracellular calcium concentration, sperm motility and induced the acrosome reaction in mature spermatozoa, and VD serum levels were positively associated with sperm motility, suggesting a role for VD in human sperm function.

Original language	English
Journal	Human Reproduction
Volume	26
Issue number	6
Pages (from-to)	1307-17
Number of pages	11
ISSN	0268-1161
DOIs	https://doi.org/10.1093/humrep/der059
Publication status	Published - 1 Jun 2011

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@article{6f7ef23d10a04654b996314b223f337f,

title = "Vitamin D is positively associated with sperm motility and increases intracellular calcium in human spermatozoa",

abstract = "BACKGROUND The vitamin D receptor (VDR) is expressed in human spermatozoa, and VDR-knockout mice and vitamin D (VD) deficiency in rodents results in impaired fertility, low sperm counts and a low number of motile spermatozoa. We investigated the role of activated VD (1,25(OH)(2)D(3)) in human spermatozoa and whether VD serum levels are associated with semen quality. METHODS Cross-sectional association study of semen quality and VD serum level in 300 men from the general population, and in vitro studies on spermatozoa from 40 men to investigate the effects of VD on intracellular calcium, sperm motility and acrosome reaction. All men delivered samples for routine semen analysis and blood for measurements of follicle stimulating hormone, Inhibin B, 25-hydroxy-VD, albumin, alkaline phosphatase, calcium and parathyroid hormone (PTH). RESULTS In the association study, 44% were VD insufficient (75 nM). 1,25(OH)(2)D(3) increased intracellular calcium concentration in human spermatozoa through VDR-mediated calcium release from an intracellular calcium storage, increased sperm motility and induced the acrosome reaction in vitro. CONCLUSIONS 1,25(OH)(2)D(3) increased intracellular calcium concentration, sperm motility and induced the acrosome reaction in mature spermatozoa, and VD serum levels were positively associated with sperm motility, suggesting a role for VD in human sperm function.",

author = "{Blomberg Jensen}, Martin and Bjerrum, {Poul J} and Jessen, {Torben E} and Nielsen, {John E} and Joensen, {Ulla N} and Olesen, {Inge A} and Petersen, {J{\o}rgen H} and Anders Juul and Steen Dissing and Niels J{\o}rgensen",

year = "2011",

month = jun,

day = "1",

doi = "10.1093/humrep/der059",

language = "English",

volume = "26",

pages = "1307--17",

journal = "Human Reproduction",

issn = "0268-1161",

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number = "6",

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TY - JOUR

T1 - Vitamin D is positively associated with sperm motility and increases intracellular calcium in human spermatozoa

AU - Blomberg Jensen, Martin

AU - Bjerrum, Poul J

AU - Jessen, Torben E

AU - Nielsen, John E

AU - Joensen, Ulla N

AU - Olesen, Inge A

AU - Petersen, Jørgen H

AU - Juul, Anders

AU - Dissing, Steen

AU - Jørgensen, Niels

PY - 2011/6/1

Y1 - 2011/6/1

N2 - BACKGROUND The vitamin D receptor (VDR) is expressed in human spermatozoa, and VDR-knockout mice and vitamin D (VD) deficiency in rodents results in impaired fertility, low sperm counts and a low number of motile spermatozoa. We investigated the role of activated VD (1,25(OH)(2)D(3)) in human spermatozoa and whether VD serum levels are associated with semen quality. METHODS Cross-sectional association study of semen quality and VD serum level in 300 men from the general population, and in vitro studies on spermatozoa from 40 men to investigate the effects of VD on intracellular calcium, sperm motility and acrosome reaction. All men delivered samples for routine semen analysis and blood for measurements of follicle stimulating hormone, Inhibin B, 25-hydroxy-VD, albumin, alkaline phosphatase, calcium and parathyroid hormone (PTH). RESULTS In the association study, 44% were VD insufficient (75 nM). 1,25(OH)(2)D(3) increased intracellular calcium concentration in human spermatozoa through VDR-mediated calcium release from an intracellular calcium storage, increased sperm motility and induced the acrosome reaction in vitro. CONCLUSIONS 1,25(OH)(2)D(3) increased intracellular calcium concentration, sperm motility and induced the acrosome reaction in mature spermatozoa, and VD serum levels were positively associated with sperm motility, suggesting a role for VD in human sperm function.

AB - BACKGROUND The vitamin D receptor (VDR) is expressed in human spermatozoa, and VDR-knockout mice and vitamin D (VD) deficiency in rodents results in impaired fertility, low sperm counts and a low number of motile spermatozoa. We investigated the role of activated VD (1,25(OH)(2)D(3)) in human spermatozoa and whether VD serum levels are associated with semen quality. METHODS Cross-sectional association study of semen quality and VD serum level in 300 men from the general population, and in vitro studies on spermatozoa from 40 men to investigate the effects of VD on intracellular calcium, sperm motility and acrosome reaction. All men delivered samples for routine semen analysis and blood for measurements of follicle stimulating hormone, Inhibin B, 25-hydroxy-VD, albumin, alkaline phosphatase, calcium and parathyroid hormone (PTH). RESULTS In the association study, 44% were VD insufficient (75 nM). 1,25(OH)(2)D(3) increased intracellular calcium concentration in human spermatozoa through VDR-mediated calcium release from an intracellular calcium storage, increased sperm motility and induced the acrosome reaction in vitro. CONCLUSIONS 1,25(OH)(2)D(3) increased intracellular calcium concentration, sperm motility and induced the acrosome reaction in mature spermatozoa, and VD serum levels were positively associated with sperm motility, suggesting a role for VD in human sperm function.

U2 - 10.1093/humrep/der059

DO - 10.1093/humrep/der059

M3 - Journal article

C2 - 21427118

SN - 0268-1161

VL - 26

SP - 1307

EP - 1317

JO - Human Reproduction

JF - Human Reproduction

IS - 6

ER -

Vitamin D is positively associated with sperm motility and increases intracellular calcium in human spermatozoa

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