Vitamin D insufficient levels during pregnancy and micronuclei frequency in peripheral blood T lymphocytes mothers and newborns (Rhea cohort, Crete)

Cristina O'Callaghan-Gordo; Manolis Kogevinas; Eleni Fthenou; Marie Pedersen; Ana Espinosa; Georgia Chalkiadaki; Vasiliki Daraki; Eirini Dermitzaki; Ilse Decordier; Vaggelis Georgiou; Domenico Franco Merlo; Theano Roumeliotaki; Kim Vande Loock; Jos Kleinjans; Micheline Kirsch-volders; Leda Chatzi

doi:10.1016/j.clnu.2016.06.016

Vitamin D insufficient levels during pregnancy and micronuclei frequency in peripheral blood T lymphocytes mothers and newborns (Rhea cohort, Crete)

Cristina O'Callaghan-Gordo, Manolis Kogevinas, Eleni Fthenou, Marie Pedersen, Ana Espinosa, Georgia Chalkiadaki, Vasiliki Daraki, Eirini Dermitzaki, Ilse Decordier, Vaggelis Georgiou, Domenico Franco Merlo, Theano Roumeliotaki, Kim Vande Loock, Jos Kleinjans, Micheline Kirsch-volders, Leda Chatzi

2 Citations (Scopus)

Abstract

Background & aims Vitamin D deficiency is common among pregnant women and may be associated with several adverse health outcomes including cancer. Micronuclei frequency is a biomarker of early genetic effects and has been used to examine the association between genotoxic exposures and cancer. We examined maternal vitamin D levels during pregnancy in associations with micronuclei frequency in maternal blood and in cord blood. Methods 173 mothers and 171 newborns born between 2007 and 2008 in Heraklion (Crete, Greece) were included in the study. Between 14th and 18th weeks of gestation we collected information on maternal diet using food frequency questionnaires (FFQs). We measured maternal serum concentrations of 25-hydroxyvitamin D [25(OH)D] between the first and second trimester of pregnancy. We estimated dietary vitamin D intake using information from FFQ. After delivery we collected cord blood and maternal peripheral blood. We used the cytokinesis-block micronucleus (CBMN) assay to assess the frequencies of micronucleated cells in binucleated T lymphocytes (MNBN). Results Maternal insufficient serum levels of 25(OH)D (<50 nmol/L) during pregnancy were associated with increased MNBN frequency in cord blood [IRR = 1.32 (95%CI: 1.00, 1.72)]. This increase was higher for newborns with birth weight above the third quartile [≥3.500 kg; IRR = 2.21 (1.26, 3.89)]. Similarly, low levels of dietary vitamin D were associated with increased MNBN frequency in cord blood [middle tertile IRR = 1.08 (0.78, 1.47), lower tertile IRR = 1.51 (1.06, 2.14)]. Insufficient levels of vitamin D were not associated with MNBN in mothers. Conclusion Our results suggest that vitamin D deficiency during pregnancy increases genotoxic risks in newborns. The prevalence of vitamin D deficiency globally is high and it is important to further investigate whether vitamin D supplementation or similar interventions during pregnancy could prevent DNA damage at early stages of life.

Original language	English
Journal	Clinical Nutrition
Pages (from-to)	1-7
Number of pages	7
ISSN	0261-5614
DOIs	https://doi.org/10.1016/j.clnu.2016.06.016
Publication status	Published - Aug 2017
Externally published	Yes

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O'Callaghan-Gordo, C., Kogevinas, M., Fthenou, E., Pedersen, M., Espinosa, A., Chalkiadaki, G., Daraki, V., Dermitzaki, E., Decordier, I., Georgiou, V., Merlo, D. F., Roumeliotaki, T., Vande Loock, K., Kleinjans, J., Kirsch-volders, M., & Chatzi, L. (2017). Vitamin D insufficient levels during pregnancy and micronuclei frequency in peripheral blood T lymphocytes mothers and newborns (Rhea cohort, Crete). Clinical Nutrition, 1-7. https://doi.org/10.1016/j.clnu.2016.06.016

O'Callaghan-Gordo, C, Kogevinas, M, Fthenou, E, Pedersen, M, Espinosa, A, Chalkiadaki, G, Daraki, V, Dermitzaki, E, Decordier, I, Georgiou, V, Merlo, DF, Roumeliotaki, T, Vande Loock, K, Kleinjans, J, Kirsch-volders, M & Chatzi, L 2017, 'Vitamin D insufficient levels during pregnancy and micronuclei frequency in peripheral blood T lymphocytes mothers and newborns (Rhea cohort, Crete)', Clinical Nutrition, pp. 1-7. https://doi.org/10.1016/j.clnu.2016.06.016

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title = "Vitamin D insufficient levels during pregnancy and micronuclei frequency in peripheral blood T lymphocytes mothers and newborns (Rhea cohort, Crete)",

abstract = "Background & aims Vitamin D deficiency is common among pregnant women and may be associated with several adverse health outcomes including cancer. Micronuclei frequency is a biomarker of early genetic effects and has been used to examine the association between genotoxic exposures and cancer. We examined maternal vitamin D levels during pregnancy in associations with micronuclei frequency in maternal blood and in cord blood. Methods 173 mothers and 171 newborns born between 2007 and 2008 in Heraklion (Crete, Greece) were included in the study. Between 14th and 18th weeks of gestation we collected information on maternal diet using food frequency questionnaires (FFQs). We measured maternal serum concentrations of 25-hydroxyvitamin D [25(OH)D] between the first and second trimester of pregnancy. We estimated dietary vitamin D intake using information from FFQ. After delivery we collected cord blood and maternal peripheral blood. We used the cytokinesis-block micronucleus (CBMN) assay to assess the frequencies of micronucleated cells in binucleated T lymphocytes (MNBN). Results Maternal insufficient serum levels of 25(OH)D (<50 nmol/L) during pregnancy were associated with increased MNBN frequency in cord blood [IRR = 1.32 (95%CI: 1.00, 1.72)]. This increase was higher for newborns with birth weight above the third quartile [≥3.500 kg; IRR = 2.21 (1.26, 3.89)]. Similarly, low levels of dietary vitamin D were associated with increased MNBN frequency in cord blood [middle tertile IRR = 1.08 (0.78, 1.47), lower tertile IRR = 1.51 (1.06, 2.14)]. Insufficient levels of vitamin D were not associated with MNBN in mothers. Conclusion Our results suggest that vitamin D deficiency during pregnancy increases genotoxic risks in newborns. The prevalence of vitamin D deficiency globally is high and it is important to further investigate whether vitamin D supplementation or similar interventions during pregnancy could prevent DNA damage at early stages of life.",

author = "Cristina O'Callaghan-Gordo and Manolis Kogevinas and Eleni Fthenou and Marie Pedersen and Ana Espinosa and Georgia Chalkiadaki and Vasiliki Daraki and Eirini Dermitzaki and Ilse Decordier and Vaggelis Georgiou and Merlo, {Domenico Franco} and Theano Roumeliotaki and {Vande Loock}, Kim and Jos Kleinjans and Micheline Kirsch-volders and Leda Chatzi",

year = "2017",

month = aug,

doi = "10.1016/j.clnu.2016.06.016",

language = "English",

pages = "1--7",

journal = "Clinical Nutrition",

issn = "0261-5614",

publisher = "Elsevier",

}

TY - JOUR

T1 - Vitamin D insufficient levels during pregnancy and micronuclei frequency in peripheral blood T lymphocytes mothers and newborns (Rhea cohort, Crete)

AU - O'Callaghan-Gordo, Cristina

AU - Kogevinas, Manolis

AU - Fthenou, Eleni

AU - Pedersen, Marie

AU - Espinosa, Ana

AU - Chalkiadaki, Georgia

AU - Daraki, Vasiliki

AU - Dermitzaki, Eirini

AU - Decordier, Ilse

AU - Georgiou, Vaggelis

AU - Merlo, Domenico Franco

AU - Roumeliotaki, Theano

AU - Vande Loock, Kim

AU - Kleinjans, Jos

AU - Kirsch-volders, Micheline

AU - Chatzi, Leda

PY - 2017/8

Y1 - 2017/8

N2 - Background & aims Vitamin D deficiency is common among pregnant women and may be associated with several adverse health outcomes including cancer. Micronuclei frequency is a biomarker of early genetic effects and has been used to examine the association between genotoxic exposures and cancer. We examined maternal vitamin D levels during pregnancy in associations with micronuclei frequency in maternal blood and in cord blood. Methods 173 mothers and 171 newborns born between 2007 and 2008 in Heraklion (Crete, Greece) were included in the study. Between 14th and 18th weeks of gestation we collected information on maternal diet using food frequency questionnaires (FFQs). We measured maternal serum concentrations of 25-hydroxyvitamin D [25(OH)D] between the first and second trimester of pregnancy. We estimated dietary vitamin D intake using information from FFQ. After delivery we collected cord blood and maternal peripheral blood. We used the cytokinesis-block micronucleus (CBMN) assay to assess the frequencies of micronucleated cells in binucleated T lymphocytes (MNBN). Results Maternal insufficient serum levels of 25(OH)D (<50 nmol/L) during pregnancy were associated with increased MNBN frequency in cord blood [IRR = 1.32 (95%CI: 1.00, 1.72)]. This increase was higher for newborns with birth weight above the third quartile [≥3.500 kg; IRR = 2.21 (1.26, 3.89)]. Similarly, low levels of dietary vitamin D were associated with increased MNBN frequency in cord blood [middle tertile IRR = 1.08 (0.78, 1.47), lower tertile IRR = 1.51 (1.06, 2.14)]. Insufficient levels of vitamin D were not associated with MNBN in mothers. Conclusion Our results suggest that vitamin D deficiency during pregnancy increases genotoxic risks in newborns. The prevalence of vitamin D deficiency globally is high and it is important to further investigate whether vitamin D supplementation or similar interventions during pregnancy could prevent DNA damage at early stages of life.

AB - Background & aims Vitamin D deficiency is common among pregnant women and may be associated with several adverse health outcomes including cancer. Micronuclei frequency is a biomarker of early genetic effects and has been used to examine the association between genotoxic exposures and cancer. We examined maternal vitamin D levels during pregnancy in associations with micronuclei frequency in maternal blood and in cord blood. Methods 173 mothers and 171 newborns born between 2007 and 2008 in Heraklion (Crete, Greece) were included in the study. Between 14th and 18th weeks of gestation we collected information on maternal diet using food frequency questionnaires (FFQs). We measured maternal serum concentrations of 25-hydroxyvitamin D [25(OH)D] between the first and second trimester of pregnancy. We estimated dietary vitamin D intake using information from FFQ. After delivery we collected cord blood and maternal peripheral blood. We used the cytokinesis-block micronucleus (CBMN) assay to assess the frequencies of micronucleated cells in binucleated T lymphocytes (MNBN). Results Maternal insufficient serum levels of 25(OH)D (<50 nmol/L) during pregnancy were associated with increased MNBN frequency in cord blood [IRR = 1.32 (95%CI: 1.00, 1.72)]. This increase was higher for newborns with birth weight above the third quartile [≥3.500 kg; IRR = 2.21 (1.26, 3.89)]. Similarly, low levels of dietary vitamin D were associated with increased MNBN frequency in cord blood [middle tertile IRR = 1.08 (0.78, 1.47), lower tertile IRR = 1.51 (1.06, 2.14)]. Insufficient levels of vitamin D were not associated with MNBN in mothers. Conclusion Our results suggest that vitamin D deficiency during pregnancy increases genotoxic risks in newborns. The prevalence of vitamin D deficiency globally is high and it is important to further investigate whether vitamin D supplementation or similar interventions during pregnancy could prevent DNA damage at early stages of life.

U2 - 10.1016/j.clnu.2016.06.016

DO - 10.1016/j.clnu.2016.06.016

M3 - Journal article

C2 - 27396287

SN - 0261-5614

SP - 1

EP - 7

JO - Clinical Nutrition

JF - Clinical Nutrition

ER -

Vitamin D insufficient levels during pregnancy and micronuclei frequency in peripheral blood T lymphocytes mothers and newborns (Rhea cohort, Crete)

Abstract

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