Visualization of interaction between inorganic nanoparticles and bacteria or fungi

André Chwalibog; Ewa Sawosz; Anna Malgorzata Hotowy; Jacek Szeliga; Stanislaw Mitura; Katarzyna  Mitura; Marta Grodzik; Piotr Orlowski; Aleksandra Sokolowska

doi:10.2147/IJN.S13532

Visualization of interaction between inorganic nanoparticles and bacteria or fungi

André Chwalibog, Ewa Sawosz, Anna Malgorzata Hotowy, Jacek Szeliga, Stanislaw Mitura, Katarzyna Mitura, Marta Grodzik, Piotr Orlowski, Aleksandra Sokolowska

175 Citations (Scopus)

Abstract

Purpose: The objective of the present investigation was to evaluate the morphologic characteristics of self-assemblies of diamond (nano-D), silver (nano-Ag), gold (nano-Au), and platinum (nano-Pt) nanoparticles with Staphylococcus aureus (bacteria) and Candida albicans (fungi), to determine the possibility of constructing microorganism-nanoparticle vehicles. Methods: Hydrocolloids of individual nanoparticles were added to suspensions of S. aureus and C. albicans. Immediately after mixing, the samples were inspected by transmission electron microscopy. Results: Visualization of the morphologic interaction between the nanoparticles and microorganisms showed that nano-D, which are dielectrics and exhibit a positive zeta potential, were very different from the membrane potentials of microorganisms, and uniformly surrounded the microorganisms, without causing visible damage and destruction of cells. All metal nanoparticles with negative zeta potential had cell damaging properties. Nano-Ag showed the properties of self-organization with the cells, disintegrating the cell walls and cytoplasmic membranes, and releasing a substance (probably cytoplasm) outside the cell. Arrangement of nano-Au with microorganisms did not create a system of self-organization, but instead a "noncontact" interaction between the nanoparticles and microorganisms was observed to cause damage to fungal cells. Nano-Pt caused both microorganisms to release a substance outside the cell and disintegrated the cytoplasmic membrane and cell wall. Conclusion: Nano-Ag, nano-Au, and nano-Pt (all metal nanoparticles) are harmful to bacteria and fungi. In contrast, nano-D bind closely to the surface of microorganisms without causing visible damage to cells, and demonstrating good self-assembling ability. The results indicate that both microorganisms could be used as potential carriers for nano-D.

Original language	English
Journal	International Journal of Nanomedicine (Online)
Volume	5
Pages (from-to)	1085-1094
Number of pages	10
ISSN	1176-9114
DOIs	https://doi.org/10.2147/IJN.S13532
Publication status	Published - 2010

Keywords

Former LIFE faculty

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10.2147/IJN.S13532

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title = "Visualization of interaction between inorganic nanoparticles and bacteria or fungi",

abstract = "Purpose: The objective of the present investigation was to evaluate the morphologic characteristics of self-assemblies of diamond (nano-D), silver (nano-Ag), gold (nano-Au), and platinum (nano-Pt) nanoparticles with Staphylococcus aureus (bacteria) and Candida albicans (fungi), to determine the possibility of constructing microorganism-nanoparticle vehicles. Methods: Hydrocolloids of individual nanoparticles were added to suspensions of S. aureus and C. albicans. Immediately after mixing, the samples were inspected by transmission electron microscopy. Results: Visualization of the morphologic interaction between the nanoparticles and microorganisms showed that nano-D, which are dielectrics and exhibit a positive zeta potential, were very different from the membrane potentials of microorganisms, and uniformly surrounded the microorganisms, without causing visible damage and destruction of cells. All metal nanoparticles with negative zeta potential had cell damaging properties. Nano-Ag showed the properties of self-organization with the cells, disintegrating the cell walls and cytoplasmic membranes, and releasing a substance (probably cytoplasm) outside the cell. Arrangement of nano-Au with microorganisms did not create a system of self-organization, but instead a {"}noncontact{"} interaction between the nanoparticles and microorganisms was observed to cause damage to fungal cells. Nano-Pt caused both microorganisms to release a substance outside the cell and disintegrated the cytoplasmic membrane and cell wall. Conclusion: Nano-Ag, nano-Au, and nano-Pt (all metal nanoparticles) are harmful to bacteria and fungi. In contrast, nano-D bind closely to the surface of microorganisms without causing visible damage to cells, and demonstrating good self-assembling ability. The results indicate that both microorganisms could be used as potential carriers for nano-D.",

keywords = "Former LIFE faculty, nanoparticles, diamon, silver, gold, platinum, Staphylococcus aureus, Candida albicans, morphology",

author = "Andr{\'e} Chwalibog and Ewa Sawosz and Hotowy, {Anna Malgorzata} and Jacek Szeliga and Stanislaw Mitura and Katarzyna Mitura and Marta Grodzik and Piotr Orlowski and Aleksandra Sokolowska",

year = "2010",

doi = "10.2147/IJN.S13532",

language = "English",

volume = "5",

pages = "1085--1094",

journal = "International Journal of Nanomedicine",

issn = "1176-9114",

publisher = "Dove Press Ltd",

}

TY - JOUR

T1 - Visualization of interaction between inorganic nanoparticles and bacteria or fungi

AU - Chwalibog, André

AU - Sawosz, Ewa

AU - Hotowy, Anna Malgorzata

AU - Szeliga, Jacek

AU - Mitura, Stanislaw

AU - Mitura, Katarzyna

AU - Grodzik, Marta

AU - Orlowski, Piotr

AU - Sokolowska, Aleksandra

PY - 2010

Y1 - 2010

N2 - Purpose: The objective of the present investigation was to evaluate the morphologic characteristics of self-assemblies of diamond (nano-D), silver (nano-Ag), gold (nano-Au), and platinum (nano-Pt) nanoparticles with Staphylococcus aureus (bacteria) and Candida albicans (fungi), to determine the possibility of constructing microorganism-nanoparticle vehicles. Methods: Hydrocolloids of individual nanoparticles were added to suspensions of S. aureus and C. albicans. Immediately after mixing, the samples were inspected by transmission electron microscopy. Results: Visualization of the morphologic interaction between the nanoparticles and microorganisms showed that nano-D, which are dielectrics and exhibit a positive zeta potential, were very different from the membrane potentials of microorganisms, and uniformly surrounded the microorganisms, without causing visible damage and destruction of cells. All metal nanoparticles with negative zeta potential had cell damaging properties. Nano-Ag showed the properties of self-organization with the cells, disintegrating the cell walls and cytoplasmic membranes, and releasing a substance (probably cytoplasm) outside the cell. Arrangement of nano-Au with microorganisms did not create a system of self-organization, but instead a "noncontact" interaction between the nanoparticles and microorganisms was observed to cause damage to fungal cells. Nano-Pt caused both microorganisms to release a substance outside the cell and disintegrated the cytoplasmic membrane and cell wall. Conclusion: Nano-Ag, nano-Au, and nano-Pt (all metal nanoparticles) are harmful to bacteria and fungi. In contrast, nano-D bind closely to the surface of microorganisms without causing visible damage to cells, and demonstrating good self-assembling ability. The results indicate that both microorganisms could be used as potential carriers for nano-D.

AB - Purpose: The objective of the present investigation was to evaluate the morphologic characteristics of self-assemblies of diamond (nano-D), silver (nano-Ag), gold (nano-Au), and platinum (nano-Pt) nanoparticles with Staphylococcus aureus (bacteria) and Candida albicans (fungi), to determine the possibility of constructing microorganism-nanoparticle vehicles. Methods: Hydrocolloids of individual nanoparticles were added to suspensions of S. aureus and C. albicans. Immediately after mixing, the samples were inspected by transmission electron microscopy. Results: Visualization of the morphologic interaction between the nanoparticles and microorganisms showed that nano-D, which are dielectrics and exhibit a positive zeta potential, were very different from the membrane potentials of microorganisms, and uniformly surrounded the microorganisms, without causing visible damage and destruction of cells. All metal nanoparticles with negative zeta potential had cell damaging properties. Nano-Ag showed the properties of self-organization with the cells, disintegrating the cell walls and cytoplasmic membranes, and releasing a substance (probably cytoplasm) outside the cell. Arrangement of nano-Au with microorganisms did not create a system of self-organization, but instead a "noncontact" interaction between the nanoparticles and microorganisms was observed to cause damage to fungal cells. Nano-Pt caused both microorganisms to release a substance outside the cell and disintegrated the cytoplasmic membrane and cell wall. Conclusion: Nano-Ag, nano-Au, and nano-Pt (all metal nanoparticles) are harmful to bacteria and fungi. In contrast, nano-D bind closely to the surface of microorganisms without causing visible damage to cells, and demonstrating good self-assembling ability. The results indicate that both microorganisms could be used as potential carriers for nano-D.

KW - Former LIFE faculty

KW - nanoparticles

KW - diamon

KW - silver

KW - gold

KW - platinum

KW - Staphylococcus aureus

KW - Candida albicans

KW - morphology

U2 - 10.2147/IJN.S13532

DO - 10.2147/IJN.S13532

M3 - Journal article

C2 - 21270959

SN - 1176-9114

VL - 5

SP - 1085

EP - 1094

JO - International Journal of Nanomedicine

JF - International Journal of Nanomedicine

ER -

Visualization of interaction between inorganic nanoparticles and bacteria or fungi

Abstract

Keywords

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