Visualization and quantitation of poly A+-mRNA by using in situ hybridization with biotinylated oligonucleotides.

K Wehner; P Fritz; K Köhler; H Multhaupt

Visualization and quantitation of poly A+-mRNA by using in situ hybridization with biotinylated oligonucleotides.

K Wehner, P Fritz, K Köhler, H Multhaupt

2 Citations (Scopus)

Abstract

HeLa cells and synovial tissue sections were used for visualizing poly A+-mRNA after in situ hybridization. We describe a method to biotinylate hybridization probes by tailing oligo-dT nucleotides with biotinyl-dUTP using terminal deoxynucleotidyl transferase (E.C.2.7.7.3.1.). The hybrids were detected by using the strepavidin-biotin-peroxidase complex (ABC method). The reaction product of the peroxidase staining in the cells and in the tissue sections, is quantitated by the method of plug- and scanning photometry respectively.

Original language	English
Journal	Acta histochemica. Supplementband
Volume	37
Pages (from-to)	83-8
Number of pages	5
ISSN	0567-7556
Publication status	Published - 1989
Externally published	Yes

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@article{a33515605d4311dd8d9f000ea68e967b,

title = "Visualization and quantitation of poly A+-mRNA by using in situ hybridization with biotinylated oligonucleotides.",

abstract = "HeLa cells and synovial tissue sections were used for visualizing poly A+-mRNA after in situ hybridization. We describe a method to biotinylate hybridization probes by tailing oligo-dT nucleotides with biotinyl-dUTP using terminal deoxynucleotidyl transferase (E.C.2.7.7.3.1.). The hybrids were detected by using the strepavidin-biotin-peroxidase complex (ABC method). The reaction product of the peroxidase staining in the cells and in the tissue sections, is quantitated by the method of plug- and scanning photometry respectively.",

author = "K Wehner and P Fritz and K K{\"o}hler and H Multhaupt",

note = "Keywords: Hela Cells; Humans; Immunoenzyme Techniques; Neoplasm Proteins; Nucleic Acid Hybridization; Oligonucleotide Probes; Poly A; RNA, Messenger",

year = "1989",

language = "English",

volume = "37",

pages = "83--8",

journal = "Acta histochemica. Supplementband",

issn = "0567-7556",

}

TY - JOUR

T1 - Visualization and quantitation of poly A+-mRNA by using in situ hybridization with biotinylated oligonucleotides.

AU - Wehner, K

AU - Fritz, P

AU - Köhler, K

AU - Multhaupt, H

N1 - Keywords: Hela Cells; Humans; Immunoenzyme Techniques; Neoplasm Proteins; Nucleic Acid Hybridization; Oligonucleotide Probes; Poly A; RNA, Messenger

PY - 1989

Y1 - 1989

N2 - HeLa cells and synovial tissue sections were used for visualizing poly A+-mRNA after in situ hybridization. We describe a method to biotinylate hybridization probes by tailing oligo-dT nucleotides with biotinyl-dUTP using terminal deoxynucleotidyl transferase (E.C.2.7.7.3.1.). The hybrids were detected by using the strepavidin-biotin-peroxidase complex (ABC method). The reaction product of the peroxidase staining in the cells and in the tissue sections, is quantitated by the method of plug- and scanning photometry respectively.

AB - HeLa cells and synovial tissue sections were used for visualizing poly A+-mRNA after in situ hybridization. We describe a method to biotinylate hybridization probes by tailing oligo-dT nucleotides with biotinyl-dUTP using terminal deoxynucleotidyl transferase (E.C.2.7.7.3.1.). The hybrids were detected by using the strepavidin-biotin-peroxidase complex (ABC method). The reaction product of the peroxidase staining in the cells and in the tissue sections, is quantitated by the method of plug- and scanning photometry respectively.

M3 - Journal article

C2 - 2505326

SN - 0567-7556

VL - 37

SP - 83

EP - 88

JO - Acta histochemica. Supplementband

JF - Acta histochemica. Supplementband

ER -

Visualization and quantitation of poly A+-mRNA by using in situ hybridization with biotinylated oligonucleotides.

Abstract

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