Vegetables and PUFA-rich plant oil reduce DNA strand breaks in individuals with type 2 diabetes

Elisabeth Müllner; Helmut Brath; Simone Pleifer; Christiane Schiermayr; Andreas Baierl; Marlies Wallner; Theresia Fastian; Yvonne Millner; Kristina Paller; Trine Henriksen; Henrik Enghusen Poulsen; Ernst Forster; Karl-Heinz Wagner

doi:10.1002/mnfr.201200343

Vegetables and PUFA-rich plant oil reduce DNA strand breaks in individuals with type 2 diabetes

Elisabeth Müllner, Helmut Brath, Simone Pleifer, Christiane Schiermayr, Andreas Baierl, Marlies Wallner, Theresia Fastian, Yvonne Millner, Kristina Paller, Trine Henriksen, Henrik Enghusen Poulsen, Ernst Forster, Karl-Heinz Wagner

30 Citations (Scopus)

Abstract

Type 2 diabetes is a multifactorial disease associated with increased oxidative stress, which may lead to increased DNA damage. The aim of this study was to investigate the effect of a healthy diet on DNA oxidation in diabetics and nondiabetics. Methods and results: Seventy-six diabetic and 21 nondiabetic individuals participated in this study. All subjects received information about the benefits of a healthy diet, while subjects randomly assigned to the intervention group received additionally 300 g of vegetables and 25 mL PUFA-rich plant oil per day. DNA damage in mononuclear cells (Comet Assay), urinary excretion of 8-oxo-7-hydro-2′-deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydroguanosine (8-oxoGuo) and glycated hemoglobin (HbA1c) were measured at baseline, after 4, 8 (end of intervention), and 16 weeks. The intervention with vegetables and PUFA-rich oil led to a significant increase in plasma antioxidant concentrations. Diabetic individuals of the intervention group showed a significant reduction in HbA1c and DNA strand breaks. Levels of HbA1c were also improved in diabetics of the information group, but oxidative damage to DNA was not altered. Urinary 8-oxodG and 8-oxoGuo excretion remained unchanged in both groups. Conclusions: This study provides evidence that a healthy diet rich in antioxidants reduces levels of DNA strand breaks in diabetic individuals.

Original language	English
Journal	Molecular Nutrition & Food Research
Volume	57
Issue number	2
Pages (from-to)	328-338
ISSN	1613-4125
DOIs	https://doi.org/10.1002/mnfr.201200343
Publication status	Published - Feb 2013

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/mnfr.201200343

Cite this

Müllner, E., Brath, H., Pleifer, S., Schiermayr, C., Baierl, A., Wallner, M., Fastian, T., Millner, Y., Paller, K., Henriksen, T., Poulsen, H. E., Forster, E., & Wagner, K-H. (2013). Vegetables and PUFA-rich plant oil reduce DNA strand breaks in individuals with type 2 diabetes. Molecular Nutrition & Food Research, 57(2), 328-338. https://doi.org/10.1002/mnfr.201200343

@article{bbb57ebb79b04ece8c34976ea14b3df6,

title = "Vegetables and PUFA-rich plant oil reduce DNA strand breaks in individuals with type 2 diabetes",

abstract = "Type 2 diabetes is a multifactorial disease associated with increased oxidative stress, which may lead to increased DNA damage. The aim of this study was to investigate the effect of a healthy diet on DNA oxidation in diabetics and nondiabetics. Methods and results: Seventy-six diabetic and 21 nondiabetic individuals participated in this study. All subjects received information about the benefits of a healthy diet, while subjects randomly assigned to the intervention group received additionally 300 g of vegetables and 25 mL PUFA-rich plant oil per day. DNA damage in mononuclear cells (Comet Assay), urinary excretion of 8-oxo-7-hydro-2′-deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydroguanosine (8-oxoGuo) and glycated hemoglobin (HbA1c) were measured at baseline, after 4, 8 (end of intervention), and 16 weeks. The intervention with vegetables and PUFA-rich oil led to a significant increase in plasma antioxidant concentrations. Diabetic individuals of the intervention group showed a significant reduction in HbA1c and DNA strand breaks. Levels of HbA1c were also improved in diabetics of the information group, but oxidative damage to DNA was not altered. Urinary 8-oxodG and 8-oxoGuo excretion remained unchanged in both groups. Conclusions: This study provides evidence that a healthy diet rich in antioxidants reduces levels of DNA strand breaks in diabetic individuals.",

author = "Elisabeth M{\"u}llner and Helmut Brath and Simone Pleifer and Christiane Schiermayr and Andreas Baierl and Marlies Wallner and Theresia Fastian and Yvonne Millner and Kristina Paller and Trine Henriksen and Poulsen, {Henrik Enghusen} and Ernst Forster and Karl-Heinz Wagner",

note = "{\textcopyright} 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",

year = "2013",

month = feb,

doi = "10.1002/mnfr.201200343",

language = "English",

volume = "57",

pages = "328--338",

journal = "Molecular Nutrition & Food Research",

issn = "1613-4125",

publisher = "Wiley-VCH",

number = "2",

}

TY - JOUR

T1 - Vegetables and PUFA-rich plant oil reduce DNA strand breaks in individuals with type 2 diabetes

AU - Müllner, Elisabeth

AU - Brath, Helmut

AU - Pleifer, Simone

AU - Schiermayr, Christiane

AU - Baierl, Andreas

AU - Wallner, Marlies

AU - Fastian, Theresia

AU - Millner, Yvonne

AU - Paller, Kristina

AU - Henriksen, Trine

AU - Poulsen, Henrik Enghusen

AU - Forster, Ernst

AU - Wagner, Karl-Heinz

PY - 2013/2

Y1 - 2013/2

N2 - Type 2 diabetes is a multifactorial disease associated with increased oxidative stress, which may lead to increased DNA damage. The aim of this study was to investigate the effect of a healthy diet on DNA oxidation in diabetics and nondiabetics. Methods and results: Seventy-six diabetic and 21 nondiabetic individuals participated in this study. All subjects received information about the benefits of a healthy diet, while subjects randomly assigned to the intervention group received additionally 300 g of vegetables and 25 mL PUFA-rich plant oil per day. DNA damage in mononuclear cells (Comet Assay), urinary excretion of 8-oxo-7-hydro-2′-deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydroguanosine (8-oxoGuo) and glycated hemoglobin (HbA1c) were measured at baseline, after 4, 8 (end of intervention), and 16 weeks. The intervention with vegetables and PUFA-rich oil led to a significant increase in plasma antioxidant concentrations. Diabetic individuals of the intervention group showed a significant reduction in HbA1c and DNA strand breaks. Levels of HbA1c were also improved in diabetics of the information group, but oxidative damage to DNA was not altered. Urinary 8-oxodG and 8-oxoGuo excretion remained unchanged in both groups. Conclusions: This study provides evidence that a healthy diet rich in antioxidants reduces levels of DNA strand breaks in diabetic individuals.

AB - Type 2 diabetes is a multifactorial disease associated with increased oxidative stress, which may lead to increased DNA damage. The aim of this study was to investigate the effect of a healthy diet on DNA oxidation in diabetics and nondiabetics. Methods and results: Seventy-six diabetic and 21 nondiabetic individuals participated in this study. All subjects received information about the benefits of a healthy diet, while subjects randomly assigned to the intervention group received additionally 300 g of vegetables and 25 mL PUFA-rich plant oil per day. DNA damage in mononuclear cells (Comet Assay), urinary excretion of 8-oxo-7-hydro-2′-deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydroguanosine (8-oxoGuo) and glycated hemoglobin (HbA1c) were measured at baseline, after 4, 8 (end of intervention), and 16 weeks. The intervention with vegetables and PUFA-rich oil led to a significant increase in plasma antioxidant concentrations. Diabetic individuals of the intervention group showed a significant reduction in HbA1c and DNA strand breaks. Levels of HbA1c were also improved in diabetics of the information group, but oxidative damage to DNA was not altered. Urinary 8-oxodG and 8-oxoGuo excretion remained unchanged in both groups. Conclusions: This study provides evidence that a healthy diet rich in antioxidants reduces levels of DNA strand breaks in diabetic individuals.

U2 - 10.1002/mnfr.201200343

DO - 10.1002/mnfr.201200343

M3 - Journal article

C2 - 23148048

SN - 1613-4125

VL - 57

SP - 328

EP - 338

JO - Molecular Nutrition & Food Research

JF - Molecular Nutrition & Food Research

IS - 2

ER -

Vegetables and PUFA-rich plant oil reduce DNA strand breaks in individuals with type 2 diabetes

Abstract

UN SDGs

Access to Document

Fingerprint

Cite this