Variation in NOD2 augments Th2- and Th17 responses to myelin basic protein in multiple sclerosis

Chris Juul Hedegaard; Christian Enevold; Finn Sellebjerg; Klaus Bendtzen; Claus Henrik Nielsen

doi:http://dx.doi.org/10.1371/journal.pone.0020253

Variation in NOD2 augments Th2- and Th17 responses to myelin basic protein in multiple sclerosis

Chris Juul Hedegaard, Christian Enevold, Finn Sellebjerg, Klaus Bendtzen, Claus Henrik Nielsen

Department of Clinical Medicine

10 Citations (Scopus)

Abstract

Variations in the gene for the nucleotide-binding oligomerisation domain (NOD) 2 have been associated with Crohn's disease but not multiple sclerosis (MS). Here we investigate the effect of three polymorphisms in the NOD2 gene (rs5743277, rs2066842 and rs5743291) on cytokine production and CD4+ T cell proliferation elicited by human myelin basic protein (MBP) in blood mononuclear cell (MNC) cultures from 29 patients with MS. No polymorphism was observed at rs5743277. No associations with the rs2066842 polymorphism were found. Concerning rs5743291, none were homozygous for the minor allele. Seven of 29 (24%) patients were heterozygous, and five of these (71%) exhibited increased MBP-induced CD4+ T cell proliferation versus four of 22 (18%), who were homozygous for the major allele (p

Original language	English
Journal	P L o S One
Volume	6
Issue number	5
Pages (from-to)	e20253
ISSN	1932-6203
DOIs	https://doi.org/10.1371/journal.pone.0020253
Publication status	Published - 2011

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title = "Variation in NOD2 augments Th2- and Th17 responses to myelin basic protein in multiple sclerosis",

abstract = "Variations in the gene for the nucleotide-binding oligomerisation domain (NOD) 2 have been associated with Crohn's disease but not multiple sclerosis (MS). Here we investigate the effect of three polymorphisms in the NOD2 gene (rs5743277, rs2066842 and rs5743291) on cytokine production and CD4+ T cell proliferation elicited by human myelin basic protein (MBP) in blood mononuclear cell (MNC) cultures from 29 patients with MS. No polymorphism was observed at rs5743277. No associations with the rs2066842 polymorphism were found. Concerning rs5743291, none were homozygous for the minor allele. Seven of 29 (24%) patients were heterozygous, and five of these (71%) exhibited increased MBP-induced CD4+ T cell proliferation versus four of 22 (18%), who were homozygous for the major allele (p",

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T1 - Variation in NOD2 augments Th2- and Th17 responses to myelin basic protein in multiple sclerosis

AU - Hedegaard, Chris Juul

AU - Enevold, Christian

AU - Sellebjerg, Finn

AU - Bendtzen, Klaus

AU - Nielsen, Claus Henrik

PY - 2011

Y1 - 2011

N2 - Variations in the gene for the nucleotide-binding oligomerisation domain (NOD) 2 have been associated with Crohn's disease but not multiple sclerosis (MS). Here we investigate the effect of three polymorphisms in the NOD2 gene (rs5743277, rs2066842 and rs5743291) on cytokine production and CD4+ T cell proliferation elicited by human myelin basic protein (MBP) in blood mononuclear cell (MNC) cultures from 29 patients with MS. No polymorphism was observed at rs5743277. No associations with the rs2066842 polymorphism were found. Concerning rs5743291, none were homozygous for the minor allele. Seven of 29 (24%) patients were heterozygous, and five of these (71%) exhibited increased MBP-induced CD4+ T cell proliferation versus four of 22 (18%), who were homozygous for the major allele (p

AB - Variations in the gene for the nucleotide-binding oligomerisation domain (NOD) 2 have been associated with Crohn's disease but not multiple sclerosis (MS). Here we investigate the effect of three polymorphisms in the NOD2 gene (rs5743277, rs2066842 and rs5743291) on cytokine production and CD4+ T cell proliferation elicited by human myelin basic protein (MBP) in blood mononuclear cell (MNC) cultures from 29 patients with MS. No polymorphism was observed at rs5743277. No associations with the rs2066842 polymorphism were found. Concerning rs5743291, none were homozygous for the minor allele. Seven of 29 (24%) patients were heterozygous, and five of these (71%) exhibited increased MBP-induced CD4+ T cell proliferation versus four of 22 (18%), who were homozygous for the major allele (p

U2 - http://dx.doi.org/10.1371/journal.pone.0020253

DO - http://dx.doi.org/10.1371/journal.pone.0020253

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VL - 6

SP - e20253

JO - PLoS Computational Biology

JF - PLoS Computational Biology

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Variation in NOD2 augments Th2- and Th17 responses to myelin basic protein in multiple sclerosis

Abstract

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