TY - JOUR
T1 - Validation of commercially available automated canine-specific immunoturbidimetric method for measuring canine C-reactive protein
AU - Hillström, Anna
AU - Hagman, Ragnvi
AU - Tvedten, Harold
AU - Kjelgaard-Hansen, Mads
N1 - © 2014 The Authors Veterinary Clinical Pathology published by Wiley Periodicals, Inc. on behalf of American Society for Veterinary Clinical Pathology and European Society for Veterinary Clinical Pathology.
PY - 2014/6
Y1 - 2014/6
N2 - Background: Measurement of C-reactive protein (CRP) is used for diagnosing and monitoring systemic inflammatory disease in canine patients. An automated human immunoturbidimetric assay has been validated for measuring canine CRP, but cross-reactivity with canine CRP is unpredictable. Objective: The purpose of the study was to validate a new automated canine-specific immunoturbidimetric CRP method (Gentian cCRP). Methods: Studies of imprecision, accuracy, prozone effect, interference, limit of quantification, and stability under different storage conditions were performed. The new method was compared with a human CRP assay previously validated for canine CRP determination. Samples from 40 healthy dogs were analyzed to establish a reference interval. Results: Total imprecision was < 2.4% for 4 tested serum pools analyzed twice daily over 10 days. The method was linear under dilution, and no prozone effect was detected at a concentration of 1200 mg/L. Recovery after spiking serum with purified canine CRP at 2 different concentrations was 123% and 116%, respectively. No interference from hemoglobin or triglycerides (10 g/L) was detected. CRP was stable for 14 days at 4°C and 22°C. In the method comparison study, there was good agreement between the validated human CRP assay and the new canine-specific assay. Healthy dogs had CRP concentrations that were less than the limit of quantification of the Gentian cCRP method (6.8 mg/L). Conclusions: The new canine-specific immunoturbidimetric CRP assay is a reliable and rapid method for measuring canine CRP, suitable for clinical use due to the option for an automated assay.
AB - Background: Measurement of C-reactive protein (CRP) is used for diagnosing and monitoring systemic inflammatory disease in canine patients. An automated human immunoturbidimetric assay has been validated for measuring canine CRP, but cross-reactivity with canine CRP is unpredictable. Objective: The purpose of the study was to validate a new automated canine-specific immunoturbidimetric CRP method (Gentian cCRP). Methods: Studies of imprecision, accuracy, prozone effect, interference, limit of quantification, and stability under different storage conditions were performed. The new method was compared with a human CRP assay previously validated for canine CRP determination. Samples from 40 healthy dogs were analyzed to establish a reference interval. Results: Total imprecision was < 2.4% for 4 tested serum pools analyzed twice daily over 10 days. The method was linear under dilution, and no prozone effect was detected at a concentration of 1200 mg/L. Recovery after spiking serum with purified canine CRP at 2 different concentrations was 123% and 116%, respectively. No interference from hemoglobin or triglycerides (10 g/L) was detected. CRP was stable for 14 days at 4°C and 22°C. In the method comparison study, there was good agreement between the validated human CRP assay and the new canine-specific assay. Healthy dogs had CRP concentrations that were less than the limit of quantification of the Gentian cCRP method (6.8 mg/L). Conclusions: The new canine-specific immunoturbidimetric CRP assay is a reliable and rapid method for measuring canine CRP, suitable for clinical use due to the option for an automated assay.
U2 - 10.1111/vcp.12150
DO - 10.1111/vcp.12150
M3 - Journal article
C2 - 24798319
SN - 1939-165X
VL - 43
SP - 235
EP - 243
JO - Veterinary clinical pathology / American Society for Veterinary Clinical Pathology
JF - Veterinary clinical pathology / American Society for Veterinary Clinical Pathology
IS - 2
ER -
