Vaccination with IL-6 analogues induces autoantibodies to IL-6 and influences experimentally induced inflammation

Pia Galle; Lene Jensen; Christina Andersson; Salvatore Cuzzocrea; Rosanna Di Paola; Ferdinando Nicoletti; Morten Svenson; Klaus Bendtzen; Allan Randrup Thomsen; Morten B Hansen

doi:10.1016/j.intimp.2007.08.026

Vaccination with IL-6 analogues induces autoantibodies to IL-6 and influences experimentally induced inflammation

Pia Galle, Lene Jensen, Christina Andersson, Salvatore Cuzzocrea, Rosanna Di Paola, Ferdinando Nicoletti, Morten Svenson, Klaus Bendtzen, Allan Randrup Thomsen, Morten B Hansen

11 Citations (Scopus)

Abstract

IL-6 is involved in inflammation and a therapeutic target. 0.1% of Danish blood donors have nanomolar plasma concentrations of polyclonal, picomolar affinity and in vitro as well as in vivo neutralizing IgG autoantibodies to IL-6 (aAb-IL-6). Such donors are assumed to be severely IL-6 deficient; yet they appear healthy and do not exhibit overt clinical or laboratory abnormalities. We induced comparable levels of aAb-IL-6 in different mouse strains by vaccination with immunogenic IL-6 analogues. We observed that the induced aAb-IL-6 protected against collagen-induced arthritis and experimental allergic encephalitis. Furthermore, aAb-IL-6 carrying mice displayed increased plasma TNFalpha concentrations upon challenge with LPS. Taken together, induction of IL-6 autoantibodies was possible in different mouse strains. The autoantibodies influenced experimental inflammation. This immunotherapeutic principle might be a viable alternative in immune competent humans suffering from disorders driven by IL-6.

Original language	English
Journal	International Immunopharmacology
Volume	7
Issue number	13
Pages (from-to)	1704-13
Number of pages	10
ISSN	1567-5769
DOIs	https://doi.org/10.1016/j.intimp.2007.08.026
Publication status	Published - 2007

Keywords

Animals
Arthritis, Experimental
Autoantibodies
Encephalomyelitis, Autoimmune, Experimental
Interleukin-6
Lipopolysaccharides
Mice
Mice, Inbred Strains
Serum Amyloid A Protein
Tumor Necrosis Factor-alpha
Vaccination

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.intimp.2007.08.026

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title = "Vaccination with IL-6 analogues induces autoantibodies to IL-6 and influences experimentally induced inflammation",

abstract = "IL-6 is involved in inflammation and a therapeutic target. 0.1% of Danish blood donors have nanomolar plasma concentrations of polyclonal, picomolar affinity and in vitro as well as in vivo neutralizing IgG autoantibodies to IL-6 (aAb-IL-6). Such donors are assumed to be severely IL-6 deficient; yet they appear healthy and do not exhibit overt clinical or laboratory abnormalities. We induced comparable levels of aAb-IL-6 in different mouse strains by vaccination with immunogenic IL-6 analogues. We observed that the induced aAb-IL-6 protected against collagen-induced arthritis and experimental allergic encephalitis. Furthermore, aAb-IL-6 carrying mice displayed increased plasma TNFalpha concentrations upon challenge with LPS. Taken together, induction of IL-6 autoantibodies was possible in different mouse strains. The autoantibodies influenced experimental inflammation. This immunotherapeutic principle might be a viable alternative in immune competent humans suffering from disorders driven by IL-6.",

keywords = "Animals, Arthritis, Experimental, Autoantibodies, Encephalomyelitis, Autoimmune, Experimental, Interleukin-6, Lipopolysaccharides, Mice, Mice, Inbred Strains, Serum Amyloid A Protein, Tumor Necrosis Factor-alpha, Vaccination",

author = "Pia Galle and Lene Jensen and Christina Andersson and Salvatore Cuzzocrea and {Di Paola}, Rosanna and Ferdinando Nicoletti and Morten Svenson and Klaus Bendtzen and Thomsen, {Allan Randrup} and Hansen, {Morten B}",

note = "Keywords: Animals; Arthritis, Experimental; Autoantibodies; Encephalomyelitis, Autoimmune, Experimental; Interleukin-6; Lipopolysaccharides; Mice; Mice, Inbred Strains; Serum Amyloid A Protein; Tumor Necrosis Factor-alpha; Vaccination",

year = "2007",

doi = "10.1016/j.intimp.2007.08.026",

language = "English",

volume = "7",

pages = "1704--13",

journal = "International Immunopharmacology",

issn = "1567-5769",

publisher = "Elsevier",

number = "13",

}

TY - JOUR

T1 - Vaccination with IL-6 analogues induces autoantibodies to IL-6 and influences experimentally induced inflammation

AU - Galle, Pia

AU - Jensen, Lene

AU - Andersson, Christina

AU - Cuzzocrea, Salvatore

AU - Di Paola, Rosanna

AU - Nicoletti, Ferdinando

AU - Svenson, Morten

AU - Bendtzen, Klaus

AU - Thomsen, Allan Randrup

AU - Hansen, Morten B

N1 - Keywords: Animals; Arthritis, Experimental; Autoantibodies; Encephalomyelitis, Autoimmune, Experimental; Interleukin-6; Lipopolysaccharides; Mice; Mice, Inbred Strains; Serum Amyloid A Protein; Tumor Necrosis Factor-alpha; Vaccination

PY - 2007

Y1 - 2007

N2 - IL-6 is involved in inflammation and a therapeutic target. 0.1% of Danish blood donors have nanomolar plasma concentrations of polyclonal, picomolar affinity and in vitro as well as in vivo neutralizing IgG autoantibodies to IL-6 (aAb-IL-6). Such donors are assumed to be severely IL-6 deficient; yet they appear healthy and do not exhibit overt clinical or laboratory abnormalities. We induced comparable levels of aAb-IL-6 in different mouse strains by vaccination with immunogenic IL-6 analogues. We observed that the induced aAb-IL-6 protected against collagen-induced arthritis and experimental allergic encephalitis. Furthermore, aAb-IL-6 carrying mice displayed increased plasma TNFalpha concentrations upon challenge with LPS. Taken together, induction of IL-6 autoantibodies was possible in different mouse strains. The autoantibodies influenced experimental inflammation. This immunotherapeutic principle might be a viable alternative in immune competent humans suffering from disorders driven by IL-6.

AB - IL-6 is involved in inflammation and a therapeutic target. 0.1% of Danish blood donors have nanomolar plasma concentrations of polyclonal, picomolar affinity and in vitro as well as in vivo neutralizing IgG autoantibodies to IL-6 (aAb-IL-6). Such donors are assumed to be severely IL-6 deficient; yet they appear healthy and do not exhibit overt clinical or laboratory abnormalities. We induced comparable levels of aAb-IL-6 in different mouse strains by vaccination with immunogenic IL-6 analogues. We observed that the induced aAb-IL-6 protected against collagen-induced arthritis and experimental allergic encephalitis. Furthermore, aAb-IL-6 carrying mice displayed increased plasma TNFalpha concentrations upon challenge with LPS. Taken together, induction of IL-6 autoantibodies was possible in different mouse strains. The autoantibodies influenced experimental inflammation. This immunotherapeutic principle might be a viable alternative in immune competent humans suffering from disorders driven by IL-6.

KW - Animals

KW - Arthritis, Experimental

KW - Autoantibodies

KW - Encephalomyelitis, Autoimmune, Experimental

KW - Interleukin-6

KW - Lipopolysaccharides

KW - Mice

KW - Mice, Inbred Strains

KW - Serum Amyloid A Protein

KW - Tumor Necrosis Factor-alpha

KW - Vaccination

U2 - 10.1016/j.intimp.2007.08.026

DO - 10.1016/j.intimp.2007.08.026

M3 - Journal article

C2 - 17996680

SN - 1567-5769

VL - 7

SP - 1704

EP - 1713

JO - International Immunopharmacology

JF - International Immunopharmacology

IS - 13

ER -

Vaccination with IL-6 analogues induces autoantibodies to IL-6 and influences experimentally induced inflammation

Abstract

Keywords

UN SDGs

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