Upscaling and automation of electrophysiology: toward high throughput screening in ion channel drug discovery

Margit Asmild; Nicholas Oswald; Karen M Krzywkowski; Søren Friis; Rasmus B Jacobsen; Dirk Reuter; Rafael Taboryski; Jonathan Kutchinsky; Ras K Vestergaard; Rikke L Schrøder; Claus B Sørensen; Morten Bech; Mads P G Korsgaard; Niels J Willumsen

Upscaling and automation of electrophysiology: toward high throughput screening in ion channel drug discovery

Margit Asmild, Nicholas Oswald, Karen M Krzywkowski, Søren Friis, Rasmus B Jacobsen, Dirk Reuter, Rafael Taboryski, Jonathan Kutchinsky, Ras K Vestergaard, Rikke L Schrøder, Claus B Sørensen, Morten Bech, Mads P G Korsgaard, Niels J Willumsen

Molecular Integrative Physiology

56 Citations (Scopus)

Abstract

Effective screening of large compound libraries in ion channel drug discovery requires the development of new electrophysiological techniques with substantially increased throughputs compared to the conventional patch clamp technique. Sophion Bioscience is aiming to meet this challenge by developing two lines of automated patch clamp products, a traditional pipette-based system called Apatchi-1, and a silicon chip-based system QPatch. The degree of automation spans from semi-automation (Apatchi-1) where a trained technician interacts with the system in a limited way, to a complete automation (QPatch 96) where the system works continuously and unattended until screening of a full compound library is completed. The performance of the systems range from medium to high throughputs.

Original language	English
Journal	Receptors and Channels
Volume	9
Issue number	1
Pages (from-to)	49-58
Number of pages	9
ISSN	1060-6823
Publication status	Published - 2003

Cite this

Asmild, M., Oswald, N., Krzywkowski, K. M., Friis, S., Jacobsen, R. B., Reuter, D., Taboryski, R., Kutchinsky, J., Vestergaard, R. K., Schrøder, R. L., Sørensen, C. B., Bech, M., Korsgaard, M. P. G., & Willumsen, N. J. (2003). Upscaling and automation of electrophysiology: toward high throughput screening in ion channel drug discovery. Receptors and Channels, 9(1), 49-58.

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abstract = "Effective screening of large compound libraries in ion channel drug discovery requires the development of new electrophysiological techniques with substantially increased throughputs compared to the conventional patch clamp technique. Sophion Bioscience is aiming to meet this challenge by developing two lines of automated patch clamp products, a traditional pipette-based system called Apatchi-1, and a silicon chip-based system QPatch. The degree of automation spans from semi-automation (Apatchi-1) where a trained technician interacts with the system in a limited way, to a complete automation (QPatch 96) where the system works continuously and unattended until screening of a full compound library is completed. The performance of the systems range from medium to high throughputs.",

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note = "Keywords: Animals; Automation; Drug Design; Electrophysiology; Ion Channels; Ions; Patch-Clamp Techniques; Silicon; Software; Time Factors",

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T1 - Upscaling and automation of electrophysiology: toward high throughput screening in ion channel drug discovery

AU - Asmild, Margit

AU - Oswald, Nicholas

AU - Krzywkowski, Karen M

AU - Friis, Søren

AU - Jacobsen, Rasmus B

AU - Reuter, Dirk

AU - Taboryski, Rafael

AU - Kutchinsky, Jonathan

AU - Vestergaard, Ras K

AU - Schrøder, Rikke L

AU - Sørensen, Claus B

AU - Bech, Morten

AU - Korsgaard, Mads P G

AU - Willumsen, Niels J

N1 - Keywords: Animals; Automation; Drug Design; Electrophysiology; Ion Channels; Ions; Patch-Clamp Techniques; Silicon; Software; Time Factors

PY - 2003

Y1 - 2003

N2 - Effective screening of large compound libraries in ion channel drug discovery requires the development of new electrophysiological techniques with substantially increased throughputs compared to the conventional patch clamp technique. Sophion Bioscience is aiming to meet this challenge by developing two lines of automated patch clamp products, a traditional pipette-based system called Apatchi-1, and a silicon chip-based system QPatch. The degree of automation spans from semi-automation (Apatchi-1) where a trained technician interacts with the system in a limited way, to a complete automation (QPatch 96) where the system works continuously and unattended until screening of a full compound library is completed. The performance of the systems range from medium to high throughputs.

AB - Effective screening of large compound libraries in ion channel drug discovery requires the development of new electrophysiological techniques with substantially increased throughputs compared to the conventional patch clamp technique. Sophion Bioscience is aiming to meet this challenge by developing two lines of automated patch clamp products, a traditional pipette-based system called Apatchi-1, and a silicon chip-based system QPatch. The degree of automation spans from semi-automation (Apatchi-1) where a trained technician interacts with the system in a limited way, to a complete automation (QPatch 96) where the system works continuously and unattended until screening of a full compound library is completed. The performance of the systems range from medium to high throughputs.

M3 - Journal article

C2 - 12825298

SN - 1060-6823

VL - 9

SP - 49

EP - 58

JO - Receptors and Channels

JF - Receptors and Channels

IS - 1

ER -

Upscaling and automation of electrophysiology: toward high throughput screening in ion channel drug discovery

Abstract

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