Unravelling the immune signature of Plasmodium falciparum transmission-reducing immunity

Will J R Stone; Joseph J Campo; André Lin Ouédraogo; Lisette Meerstein-Kessel; Isabelle Morlais; Dari Da; Anna Cohuet; Sandrine Nsango; Colin J Sutherland; Marga van de Vegte-Bolmer; Rianne Siebelink-Stoter; Geert-Jan van Gemert; Wouter Graumans; Kjerstin Lanke; Adam D Shandling; Jozelyn V Pablo; Andy A Teng; Sophie Jones; Roos M de Jong; Amanda Fabra-García; John Bradley; Will Roeffen; Edwin Lasonder; Giuliana Gremo; Evelin Schwarzer; Chris J Janse; Susheel K Singh; Michael Theisen; Phil Felgner; Matthias Marti; Chris Drakeley; Robert Sauerwein; Teun Bousema; Matthijs M Jore

doi:10.1038/s41467-017-02646-2

Unravelling the immune signature of Plasmodium falciparum transmission-reducing immunity

Will J R Stone, Joseph J Campo, André Lin Ouédraogo, Lisette Meerstein-Kessel, Isabelle Morlais, Dari Da, Anna Cohuet, Sandrine Nsango, Colin J Sutherland, Marga van de Vegte-Bolmer, Rianne Siebelink-Stoter, Geert-Jan van Gemert, Wouter Graumans, Kjerstin Lanke, Adam D Shandling, Jozelyn V Pablo, Andy A Teng, Sophie Jones, Roos M de Jong, Amanda Fabra-GarcíaJohn Bradley, Will Roeffen, Edwin Lasonder, Giuliana Gremo, Evelin Schwarzer, Chris J Janse, Susheel K Singh, Michael Theisen, Phil Felgner, Matthias Marti, Chris Drakeley, Robert Sauerwein, Teun Bousema, Matthijs M Jore

39 Citations (Scopus)

48 Downloads (Pure)

Abstract

Infection with Plasmodium can elicit antibodies that inhibit parasite survival in the mosquito, when they are ingested in an infectious blood meal. Here, we determine the transmission-reducing activity (TRA) of naturally acquired antibodies from 648 malaria-exposed individuals using lab-based mosquito-feeding assays. Transmission inhibition is significantly associated with antibody responses to Pfs48/45, Pfs230, and to 43 novel gametocyte proteins assessed by protein microarray. In field-based mosquito-feeding assays the likelihood and rate of mosquito infection are significantly lower for individuals reactive to Pfs48/45, Pfs230 or to combinations of the novel TRA-associated proteins. We also show that naturally acquired purified antibodies against key transmission-blocking epitopes of Pfs48/45 and Pfs230 are mechanistically involved in TRA, whereas sera depleted of these antibodies retain high-level, complement-independent TRA. Our analysis demonstrates that host antibody responses to gametocyte proteins are associated with reduced malaria transmission efficiency from humans to mosquitoes.

Original language	English
Article number	558
Journal	Nature Communications
Volume	9
Issue number	1
Number of pages	14
ISSN	2041-1723
DOIs	https://doi.org/10.1038/s41467-017-02646-2
Publication status	Published - 8 Feb 2018

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1038/s41467-017-02646-2Licence: CC BY

Unravelling the immune signature of Plasmodium falciparum transmission-reducing immunityFinal published version, 2.4 MBLicence: CC BY

Cite this

Stone, W. J. R., Campo, J. J., Ouédraogo, A. L., Meerstein-Kessel, L., Morlais, I., Da, D., Cohuet, A., Nsango, S., Sutherland, C. J., van de Vegte-Bolmer, M., Siebelink-Stoter, R., van Gemert, G.-J., Graumans, W., Lanke, K., Shandling, A. D., Pablo, J. V., Teng, A. A., Jones, S., de Jong, R. M., ... Jore, M. M. (2018). Unravelling the immune signature of Plasmodium falciparum transmission-reducing immunity. Nature Communications, 9(1), Article 558. https://doi.org/10.1038/s41467-017-02646-2

Stone, WJR, Campo, JJ, Ouédraogo, AL, Meerstein-Kessel, L, Morlais, I, Da, D, Cohuet, A, Nsango, S, Sutherland, CJ, van de Vegte-Bolmer, M, Siebelink-Stoter, R, van Gemert, G-J, Graumans, W, Lanke, K, Shandling, AD, Pablo, JV, Teng, AA, Jones, S, de Jong, RM, Fabra-García, A, Bradley, J, Roeffen, W, Lasonder, E, Gremo, G, Schwarzer, E, Janse, CJ, Singh, SK , Theisen, M, Felgner, P, Marti, M, Drakeley, C, Sauerwein, R, Bousema, T & Jore, MM 2018, 'Unravelling the immune signature of Plasmodium falciparum transmission-reducing immunity', Nature Communications, vol. 9, no. 1, 558. https://doi.org/10.1038/s41467-017-02646-2

@article{facd0f52edcc4606a09b15241018e730,

title = "Unravelling the immune signature of Plasmodium falciparum transmission-reducing immunity",

abstract = "Infection with Plasmodium can elicit antibodies that inhibit parasite survival in the mosquito, when they are ingested in an infectious blood meal. Here, we determine the transmission-reducing activity (TRA) of naturally acquired antibodies from 648 malaria-exposed individuals using lab-based mosquito-feeding assays. Transmission inhibition is significantly associated with antibody responses to Pfs48/45, Pfs230, and to 43 novel gametocyte proteins assessed by protein microarray. In field-based mosquito-feeding assays the likelihood and rate of mosquito infection are significantly lower for individuals reactive to Pfs48/45, Pfs230 or to combinations of the novel TRA-associated proteins. We also show that naturally acquired purified antibodies against key transmission-blocking epitopes of Pfs48/45 and Pfs230 are mechanistically involved in TRA, whereas sera depleted of these antibodies retain high-level, complement-independent TRA. Our analysis demonstrates that host antibody responses to gametocyte proteins are associated with reduced malaria transmission efficiency from humans to mosquitoes.",

author = "Stone, {Will J R} and Campo, {Joseph J} and Ou{\'e}draogo, {Andr{\'e} Lin} and Lisette Meerstein-Kessel and Isabelle Morlais and Dari Da and Anna Cohuet and Sandrine Nsango and Sutherland, {Colin J} and {van de Vegte-Bolmer}, Marga and Rianne Siebelink-Stoter and {van Gemert}, Geert-Jan and Wouter Graumans and Kjerstin Lanke and Shandling, {Adam D} and Pablo, {Jozelyn V} and Teng, {Andy A} and Sophie Jones and {de Jong}, {Roos M} and Amanda Fabra-Garc{\'i}a and John Bradley and Will Roeffen and Edwin Lasonder and Giuliana Gremo and Evelin Schwarzer and Janse, {Chris J} and Singh, {Susheel K} and Michael Theisen and Phil Felgner and Matthias Marti and Chris Drakeley and Robert Sauerwein and Teun Bousema and Jore, {Matthijs M}",

year = "2018",

month = feb,

day = "8",

doi = "10.1038/s41467-017-02646-2",

language = "English",

volume = "9",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "nature publishing group",

number = "1",

}

TY - JOUR

T1 - Unravelling the immune signature of Plasmodium falciparum transmission-reducing immunity

AU - Stone, Will J R

AU - Campo, Joseph J

AU - Ouédraogo, André Lin

AU - Meerstein-Kessel, Lisette

AU - Morlais, Isabelle

AU - Da, Dari

AU - Cohuet, Anna

AU - Nsango, Sandrine

AU - Sutherland, Colin J

AU - van de Vegte-Bolmer, Marga

AU - Siebelink-Stoter, Rianne

AU - van Gemert, Geert-Jan

AU - Graumans, Wouter

AU - Lanke, Kjerstin

AU - Shandling, Adam D

AU - Pablo, Jozelyn V

AU - Teng, Andy A

AU - Jones, Sophie

AU - de Jong, Roos M

AU - Fabra-García, Amanda

AU - Bradley, John

AU - Roeffen, Will

AU - Lasonder, Edwin

AU - Gremo, Giuliana

AU - Schwarzer, Evelin

AU - Janse, Chris J

AU - Singh, Susheel K

AU - Theisen, Michael

AU - Felgner, Phil

AU - Marti, Matthias

AU - Drakeley, Chris

AU - Sauerwein, Robert

AU - Bousema, Teun

AU - Jore, Matthijs M

PY - 2018/2/8

Y1 - 2018/2/8

N2 - Infection with Plasmodium can elicit antibodies that inhibit parasite survival in the mosquito, when they are ingested in an infectious blood meal. Here, we determine the transmission-reducing activity (TRA) of naturally acquired antibodies from 648 malaria-exposed individuals using lab-based mosquito-feeding assays. Transmission inhibition is significantly associated with antibody responses to Pfs48/45, Pfs230, and to 43 novel gametocyte proteins assessed by protein microarray. In field-based mosquito-feeding assays the likelihood and rate of mosquito infection are significantly lower for individuals reactive to Pfs48/45, Pfs230 or to combinations of the novel TRA-associated proteins. We also show that naturally acquired purified antibodies against key transmission-blocking epitopes of Pfs48/45 and Pfs230 are mechanistically involved in TRA, whereas sera depleted of these antibodies retain high-level, complement-independent TRA. Our analysis demonstrates that host antibody responses to gametocyte proteins are associated with reduced malaria transmission efficiency from humans to mosquitoes.

AB - Infection with Plasmodium can elicit antibodies that inhibit parasite survival in the mosquito, when they are ingested in an infectious blood meal. Here, we determine the transmission-reducing activity (TRA) of naturally acquired antibodies from 648 malaria-exposed individuals using lab-based mosquito-feeding assays. Transmission inhibition is significantly associated with antibody responses to Pfs48/45, Pfs230, and to 43 novel gametocyte proteins assessed by protein microarray. In field-based mosquito-feeding assays the likelihood and rate of mosquito infection are significantly lower for individuals reactive to Pfs48/45, Pfs230 or to combinations of the novel TRA-associated proteins. We also show that naturally acquired purified antibodies against key transmission-blocking epitopes of Pfs48/45 and Pfs230 are mechanistically involved in TRA, whereas sera depleted of these antibodies retain high-level, complement-independent TRA. Our analysis demonstrates that host antibody responses to gametocyte proteins are associated with reduced malaria transmission efficiency from humans to mosquitoes.

UR - https://www.nature.com/articles/s41467-018-03769-w

U2 - 10.1038/s41467-017-02646-2

DO - 10.1038/s41467-017-02646-2

M3 - Journal article

C2 - 29422648

SN - 2041-1723

VL - 9

JO - Nature Communications

JF - Nature Communications

IS - 1

M1 - 558

ER -

Unravelling the immune signature of Plasmodium falciparum transmission-reducing immunity

Abstract

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this