Unravelling the immune signature of Plasmodium falciparum transmission-reducing immunity

Will J R Stone; Joseph J Campo; André Lin Ouédraogo; Lisette Meerstein-Kessel; Isabelle Morlais; Dari Da; Anna Cohuet; Sandrine Nsango; Colin J Sutherland; Marga van de Vegte-Bolmer; Rianne Siebelink-Stoter; Geert-Jan van Gemert; Wouter Graumans; Kjerstin Lanke; Adam D Shandling; Jozelyn V Pablo; Andy A Teng; Sophie Jones; Roos M de Jong; Amanda Fabra-García; John Bradley; Will Roeffen; Edwin Lasonder; Giuliana Gremo; Evelin Schwarzer; Chris J Janse; Susheel K Singh; Michael Theisen; Phil Felgner; Matthias Marti; Chris Drakeley; Robert Sauerwein; Teun Bousema; Matthijs M Jore

doi:10.1038/s41467-017-02646-2

Unravelling the immune signature of Plasmodium falciparum transmission-reducing immunity

Will J R Stone, Joseph J Campo, André Lin Ouédraogo, Lisette Meerstein-Kessel, Isabelle Morlais, Dari Da, Anna Cohuet, Sandrine Nsango, Colin J Sutherland, Marga van de Vegte-Bolmer, Rianne Siebelink-Stoter, Geert-Jan van Gemert, Wouter Graumans, Kjerstin Lanke, Adam D Shandling, Jozelyn V Pablo, Andy A Teng, Sophie Jones, Roos M de Jong, Amanda Fabra-GarcíaJohn Bradley, Will Roeffen, Edwin Lasonder, Giuliana Gremo, Evelin Schwarzer, Chris J Janse, Susheel K Singh, Michael Theisen, Phil Felgner, Matthias Marti, Chris Drakeley, Robert Sauerwein, Teun Bousema, Matthijs M Jore

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Abstract

Infection with Plasmodium can elicit antibodies that inhibit parasite survival in the mosquito, when they are ingested in an infectious blood meal. Here, we determine the transmission-reducing activity (TRA) of naturally acquired antibodies from 648 malaria-exposed individuals using lab-based mosquito-feeding assays. Transmission inhibition is significantly associated with antibody responses to Pfs48/45, Pfs230, and to 43 novel gametocyte proteins assessed by protein microarray. In field-based mosquito-feeding assays the likelihood and rate of mosquito infection are significantly lower for individuals reactive to Pfs48/45, Pfs230 or to combinations of the novel TRA-associated proteins. We also show that naturally acquired purified antibodies against key transmission-blocking epitopes of Pfs48/45 and Pfs230 are mechanistically involved in TRA, whereas sera depleted of these antibodies retain high-level, complement-independent TRA. Our analysis demonstrates that host antibody responses to gametocyte proteins are associated with reduced malaria transmission efficiency from humans to mosquitoes.

Original language	English
Article number	558
Journal	Nature Communications
Volume	9
Issue number	1
Number of pages	14
ISSN	2041-1723
DOIs	https://doi.org/10.1038/s41467-017-02646-2
Publication status	Published - 8 Feb 2018

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Unravelling the immune signature of Plasmodium falciparum transmission-reducing immunityFinal published version, 2.4 MBLicence: CC BY

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Stone, W. J. R., Campo, J. J., Ouédraogo, A. L., Meerstein-Kessel, L., Morlais, I., Da, D., Cohuet, A., Nsango, S., Sutherland, C. J., van de Vegte-Bolmer, M., Siebelink-Stoter, R., van Gemert, G-J., Graumans, W., Lanke, K., Shandling, A. D., Pablo, J. V., Teng, A. A., Jones, S., de Jong, R. M., ... Jore, M. M. (2018). Unravelling the immune signature of Plasmodium falciparum transmission-reducing immunity. Nature Communications, 9(1), [558]. https://doi.org/10.1038/s41467-017-02646-2

Stone, WJR, Campo, JJ, Ouédraogo, AL, Meerstein-Kessel, L, Morlais, I, Da, D, Cohuet, A, Nsango, S, Sutherland, CJ, van de Vegte-Bolmer, M, Siebelink-Stoter, R, van Gemert, G-J, Graumans, W, Lanke, K, Shandling, AD, Pablo, JV, Teng, AA, Jones, S, de Jong, RM, Fabra-García, A, Bradley, J, Roeffen, W, Lasonder, E, Gremo, G, Schwarzer, E, Janse, CJ, Singh, SK , Theisen, M, Felgner, P, Marti, M, Drakeley, C, Sauerwein, R, Bousema, T & Jore, MM 2018, 'Unravelling the immune signature of Plasmodium falciparum transmission-reducing immunity', Nature Communications, vol. 9, no. 1, 558. https://doi.org/10.1038/s41467-017-02646-2

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abstract = "Infection with Plasmodium can elicit antibodies that inhibit parasite survival in the mosquito, when they are ingested in an infectious blood meal. Here, we determine the transmission-reducing activity (TRA) of naturally acquired antibodies from 648 malaria-exposed individuals using lab-based mosquito-feeding assays. Transmission inhibition is significantly associated with antibody responses to Pfs48/45, Pfs230, and to 43 novel gametocyte proteins assessed by protein microarray. In field-based mosquito-feeding assays the likelihood and rate of mosquito infection are significantly lower for individuals reactive to Pfs48/45, Pfs230 or to combinations of the novel TRA-associated proteins. We also show that naturally acquired purified antibodies against key transmission-blocking epitopes of Pfs48/45 and Pfs230 are mechanistically involved in TRA, whereas sera depleted of these antibodies retain high-level, complement-independent TRA. Our analysis demonstrates that host antibody responses to gametocyte proteins are associated with reduced malaria transmission efficiency from humans to mosquitoes.",

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AU - van Gemert, Geert-Jan

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AU - Shandling, Adam D

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AU - Jones, Sophie

AU - de Jong, Roos M

AU - Fabra-García, Amanda

AU - Bradley, John

AU - Roeffen, Will

AU - Lasonder, Edwin

AU - Gremo, Giuliana

AU - Schwarzer, Evelin

AU - Janse, Chris J

AU - Singh, Susheel K

AU - Theisen, Michael

AU - Felgner, Phil

AU - Marti, Matthias

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N2 - Infection with Plasmodium can elicit antibodies that inhibit parasite survival in the mosquito, when they are ingested in an infectious blood meal. Here, we determine the transmission-reducing activity (TRA) of naturally acquired antibodies from 648 malaria-exposed individuals using lab-based mosquito-feeding assays. Transmission inhibition is significantly associated with antibody responses to Pfs48/45, Pfs230, and to 43 novel gametocyte proteins assessed by protein microarray. In field-based mosquito-feeding assays the likelihood and rate of mosquito infection are significantly lower for individuals reactive to Pfs48/45, Pfs230 or to combinations of the novel TRA-associated proteins. We also show that naturally acquired purified antibodies against key transmission-blocking epitopes of Pfs48/45 and Pfs230 are mechanistically involved in TRA, whereas sera depleted of these antibodies retain high-level, complement-independent TRA. Our analysis demonstrates that host antibody responses to gametocyte proteins are associated with reduced malaria transmission efficiency from humans to mosquitoes.

AB - Infection with Plasmodium can elicit antibodies that inhibit parasite survival in the mosquito, when they are ingested in an infectious blood meal. Here, we determine the transmission-reducing activity (TRA) of naturally acquired antibodies from 648 malaria-exposed individuals using lab-based mosquito-feeding assays. Transmission inhibition is significantly associated with antibody responses to Pfs48/45, Pfs230, and to 43 novel gametocyte proteins assessed by protein microarray. In field-based mosquito-feeding assays the likelihood and rate of mosquito infection are significantly lower for individuals reactive to Pfs48/45, Pfs230 or to combinations of the novel TRA-associated proteins. We also show that naturally acquired purified antibodies against key transmission-blocking epitopes of Pfs48/45 and Pfs230 are mechanistically involved in TRA, whereas sera depleted of these antibodies retain high-level, complement-independent TRA. Our analysis demonstrates that host antibody responses to gametocyte proteins are associated with reduced malaria transmission efficiency from humans to mosquitoes.

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Unravelling the immune signature of Plasmodium falciparum transmission-reducing immunity

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