Understanding the activation mechanism of Thermomyces lanuginosus lipase using rational design and tryptophan-induced fluorescence quenching

Jakob Skjold-Jørgensen, Jesper Vind, Allan Svendsen, Morten Jannik Bjerrum

6 Citations (Scopus)
Original languageEnglish
JournalEuropean Journal of Lipid Science and Technology
Volume118
Issue number11
Pages (from-to)1644-1660
Number of pages17
ISSN1438-7697
DOIs
Publication statusPublished - 1 Nov 2016

Keywords

  • Fluorescence spectroscopy
  • Lid interfacial activation
  • Lipases
  • Polarity-driven activation
  • Rational design
  • Steady state fluorescence
  • Solvent polarity
  • Triacylglycerol hydrolases
  • Tryptophan-induced quenching

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