Abstract
Aurora A is a serine/threonine kinase that is essential for a wide variety of cell-cycle-related events, but only a small number of its substrates are known. We present and validate a strategy by which to identify Aurora A substrates and their phosphorylation sites. We developed a computational approach integrating various types of biological information to generate a list of 90 potential Aurora substrates, with a prediction accuracy of about 80%. We also demonstrated the specific phosphorylation of NUSAP (nucleolar and spindle-associated protein) by Aurora A in vivo. Our results provide a means by which to develop an understanding of Aurora A function and suggest unexpected roles for this kinase.
| Original language | English |
|---|---|
| Journal | EMBO Reports |
| Volume | 11 |
| Issue number | 12 |
| Pages (from-to) | 977-84 |
| Number of pages | 8 |
| ISSN | 1469-221X |
| DOIs | |
| Publication status | Published - Dec 2010 |
Keywords
- Amino Acid Sequence
- Aurora Kinases
- Computational Biology
- Humans
- Microtubule-Associated Proteins/chemistry
- Mitosis
- Models, Biological
- Molecular Sequence Data
- Phosphorylation
- Phosphoserine/metabolism
- Protein-Serine-Threonine Kinases/metabolism
- Reproducibility of Results
- Substrate Specificity