Uncovering new substrates for Aurora A kinase

Teresa Sardon; Roland A Pache; Amelie Stein; Henrik Molina; Isabelle Vernos; Patrick Aloy

doi:10.1038/embor.2010.171

Uncovering new substrates for Aurora A kinase

Teresa Sardon, Roland A Pache, Amelie Stein, Henrik Molina, Isabelle Vernos, Patrick Aloy

Biomolecular Sciences

43 Citations (Scopus)

Abstract

Aurora A is a serine/threonine kinase that is essential for a wide variety of cell-cycle-related events, but only a small number of its substrates are known. We present and validate a strategy by which to identify Aurora A substrates and their phosphorylation sites. We developed a computational approach integrating various types of biological information to generate a list of 90 potential Aurora substrates, with a prediction accuracy of about 80%. We also demonstrated the specific phosphorylation of NUSAP (nucleolar and spindle-associated protein) by Aurora A in vivo. Our results provide a means by which to develop an understanding of Aurora A function and suggest unexpected roles for this kinase.

Original language	English
Journal	EMBO Reports
Volume	11
Issue number	12
Pages (from-to)	977-84
Number of pages	8
ISSN	1469-221X
DOIs	https://doi.org/10.1038/embor.2010.171
Publication status	Published - Dec 2010

Keywords

Amino Acid Sequence
Aurora Kinases
Computational Biology
Humans
Microtubule-Associated Proteins/chemistry
Mitosis
Models, Biological
Molecular Sequence Data
Phosphorylation
Phosphoserine/metabolism
Protein-Serine-Threonine Kinases/metabolism
Reproducibility of Results
Substrate Specificity

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10.1038/embor.2010.171

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title = "Uncovering new substrates for Aurora A kinase",

abstract = "Aurora A is a serine/threonine kinase that is essential for a wide variety of cell-cycle-related events, but only a small number of its substrates are known. We present and validate a strategy by which to identify Aurora A substrates and their phosphorylation sites. We developed a computational approach integrating various types of biological information to generate a list of 90 potential Aurora substrates, with a prediction accuracy of about 80%. We also demonstrated the specific phosphorylation of NUSAP (nucleolar and spindle-associated protein) by Aurora A in vivo. Our results provide a means by which to develop an understanding of Aurora A function and suggest unexpected roles for this kinase.",

keywords = "Amino Acid Sequence, Aurora Kinases, Computational Biology, Humans, Microtubule-Associated Proteins/chemistry, Mitosis, Models, Biological, Molecular Sequence Data, Phosphorylation, Phosphoserine/metabolism, Protein-Serine-Threonine Kinases/metabolism, Reproducibility of Results, Substrate Specificity",

author = "Teresa Sardon and Pache, {Roland A} and Amelie Stein and Henrik Molina and Isabelle Vernos and Patrick Aloy",

year = "2010",

month = dec,

doi = "10.1038/embor.2010.171",

language = "English",

volume = "11",

pages = "977--84",

journal = "E M B O Reports",

issn = "1469-221X",

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TY - JOUR

T1 - Uncovering new substrates for Aurora A kinase

AU - Sardon, Teresa

AU - Pache, Roland A

AU - Stein, Amelie

AU - Molina, Henrik

AU - Vernos, Isabelle

AU - Aloy, Patrick

PY - 2010/12

Y1 - 2010/12

N2 - Aurora A is a serine/threonine kinase that is essential for a wide variety of cell-cycle-related events, but only a small number of its substrates are known. We present and validate a strategy by which to identify Aurora A substrates and their phosphorylation sites. We developed a computational approach integrating various types of biological information to generate a list of 90 potential Aurora substrates, with a prediction accuracy of about 80%. We also demonstrated the specific phosphorylation of NUSAP (nucleolar and spindle-associated protein) by Aurora A in vivo. Our results provide a means by which to develop an understanding of Aurora A function and suggest unexpected roles for this kinase.

AB - Aurora A is a serine/threonine kinase that is essential for a wide variety of cell-cycle-related events, but only a small number of its substrates are known. We present and validate a strategy by which to identify Aurora A substrates and their phosphorylation sites. We developed a computational approach integrating various types of biological information to generate a list of 90 potential Aurora substrates, with a prediction accuracy of about 80%. We also demonstrated the specific phosphorylation of NUSAP (nucleolar and spindle-associated protein) by Aurora A in vivo. Our results provide a means by which to develop an understanding of Aurora A function and suggest unexpected roles for this kinase.

KW - Amino Acid Sequence

KW - Aurora Kinases

KW - Computational Biology

KW - Humans

KW - Microtubule-Associated Proteins/chemistry

KW - Mitosis

KW - Models, Biological

KW - Molecular Sequence Data

KW - Phosphorylation

KW - Phosphoserine/metabolism

KW - Protein-Serine-Threonine Kinases/metabolism

KW - Reproducibility of Results

KW - Substrate Specificity

U2 - 10.1038/embor.2010.171

DO - 10.1038/embor.2010.171

M3 - Journal article

C2 - 21072059

SN - 1469-221X

VL - 11

SP - 977

EP - 984

JO - E M B O Reports

JF - E M B O Reports

IS - 12

ER -

Uncovering new substrates for Aurora A kinase

Abstract

Keywords

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