Ultrastructural study and description of Cystosporogenes deliaradicae n. sp. (microspora, glugeidae), a microsporidian parasite of the cabbage root fly Delia radicum (Linnaeus, 1758) (diptera, anthomyidae)

J. I.Ronny Larsson; Jørgen Eilenberg; Jose Bresciani

doi:10.1016/S0932-4739(11)80091-X

Ultrastructural study and description of Cystosporogenes deliaradicae n. sp. (microspora, glugeidae), a microsporidian parasite of the cabbage root fly Delia radicum (Linnaeus, 1758) (diptera, anthomyidae)

J. I.Ronny Larsson^*, Jørgen Eilenberg, Jose Bresciani

^*Corresponding author for this work

Section for Organismal Biology

4 Citations (Scopus)

Abstract

The microsporidium Cystosporogenes deliaradicae sp. no v., a parasite of the adipose tissue of adult cabbage root flies, Delia radicum in Denmark, is described based on light microscopic and ultrastructural characteristics. All life cycle stages have isolated nuclei. The sporogony is polysporoblastic, yielding 4–16 (most commonly 6 or 8) ovocylindrical spores, measuring 1.2–1.5 × 4.6–5.5 μm when fixed and stained. The spore wall has an approximately 43 nm thick, layered exospore with a median layer resembling a double membrane. The polaroplast is uniformly lamellar. The isofilar, 114–123 nm thick, polar filament is arranged in 11–15 (most commonly 12–13) coils in a single layer close to the spore wall. The angle of tilt is approximately 35°. The last coil touches the posterior pole of the spore. A sporophorous vesicle is produced by the sporont. The envelope is generated as a duplication of the plasma membrane. Typically the vesicle primordia grow from one pole of the sporont to the other. The episporontal space initially has granular inclusions. Later septate, persistent tubules of exospore material appear. Simultaneously with the release of sporoblasts, wide tubulus-like, non-persistent structures are formed. They are covered by regularly spaced electron-dense material. Anomalous sporogony was frequently observed, probably caused by a simultaneous parasitism by the fungus Strongwellsea castrans. The systematic position of the microsporidium is discussed, including the reasons for incorporating the genus into the family Glugeidae.

Original language	English
Journal	European Journal of Protistology
Volume	31
Issue number	3
Pages (from-to)	275-285
Number of pages	11
ISSN	0932-4739
DOIs	https://doi.org/10.1016/S0932-4739(11)80091-X
Publication status	Published - 1 Jan 1995

Keywords

A
anchoring disc
Cystosporogenes deliaradicae sp. nov.
D
Delia radicum, adults
double layer
E
endospore
EX
exospore
F
G
Golgi vesicles
HN
host nucleus
Microspora
N
nucleus
P
plasma membrane
PM
polar filament
polar sac
polaroplast
posterior vacuole
PS
PV
S
spore
sporophorous vesicle
T
Taxonomy
tubular inclusion
Ultrastructure
V

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Ultrastructural study and description of Cystosporogenes deliaradicae n. sp. (microspora, glugeidae), a microsporidian parasite of the cabbage root fly Delia radicum (Linnaeus, 1758) (diptera, anthomyidae). / Larsson, J. I.Ronny; Eilenberg, Jørgen; Bresciani, Jose.

In: European Journal of Protistology, Vol. 31, No. 3, 01.01.1995, p. 275-285.

Research output: Contribution to journal › Journal article › Research › peer-review

@article{83954f3de76f43a6a039bb5dcf85c548,

title = "Ultrastructural study and description of Cystosporogenes deliaradicae n. sp. (microspora, glugeidae), a microsporidian parasite of the cabbage root fly Delia radicum (Linnaeus, 1758) (diptera, anthomyidae)",

abstract = "The microsporidium Cystosporogenes deliaradicae sp. no v., a parasite of the adipose tissue of adult cabbage root flies, Delia radicum in Denmark, is described based on light microscopic and ultrastructural characteristics. All life cycle stages have isolated nuclei. The sporogony is polysporoblastic, yielding 4–16 (most commonly 6 or 8) ovocylindrical spores, measuring 1.2–1.5 × 4.6–5.5 μm when fixed and stained. The spore wall has an approximately 43 nm thick, layered exospore with a median layer resembling a double membrane. The polaroplast is uniformly lamellar. The isofilar, 114–123 nm thick, polar filament is arranged in 11–15 (most commonly 12–13) coils in a single layer close to the spore wall. The angle of tilt is approximately 35°. The last coil touches the posterior pole of the spore. A sporophorous vesicle is produced by the sporont. The envelope is generated as a duplication of the plasma membrane. Typically the vesicle primordia grow from one pole of the sporont to the other. The episporontal space initially has granular inclusions. Later septate, persistent tubules of exospore material appear. Simultaneously with the release of sporoblasts, wide tubulus-like, non-persistent structures are formed. They are covered by regularly spaced electron-dense material. Anomalous sporogony was frequently observed, probably caused by a simultaneous parasitism by the fungus Strongwellsea castrans. The systematic position of the microsporidium is discussed, including the reasons for incorporating the genus into the family Glugeidae.",

keywords = "A, anchoring disc, Cystosporogenes deliaradicae sp. nov., D, Delia radicum, adults, double layer, E, endospore, EX, exospore, F, G, Golgi vesicles, HN, host nucleus, Microspora, N, nucleus, P, plasma membrane, PM, polar filament, polar sac, polaroplast, posterior vacuole, PS, PV, S, spore, sporophorous vesicle, T, Taxonomy, tubular inclusion, Ultrastructure, V",

author = "Larsson, {J. I.Ronny} and J{\o}rgen Eilenberg and Jose Bresciani",

year = "1995",

month = jan,

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doi = "10.1016/S0932-4739(11)80091-X",

language = "English",

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pages = "275--285",

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T1 - Ultrastructural study and description of Cystosporogenes deliaradicae n. sp. (microspora, glugeidae), a microsporidian parasite of the cabbage root fly Delia radicum (Linnaeus, 1758) (diptera, anthomyidae)

AU - Larsson, J. I.Ronny

AU - Eilenberg, Jørgen

AU - Bresciani, Jose

PY - 1995/1/1

Y1 - 1995/1/1

N2 - The microsporidium Cystosporogenes deliaradicae sp. no v., a parasite of the adipose tissue of adult cabbage root flies, Delia radicum in Denmark, is described based on light microscopic and ultrastructural characteristics. All life cycle stages have isolated nuclei. The sporogony is polysporoblastic, yielding 4–16 (most commonly 6 or 8) ovocylindrical spores, measuring 1.2–1.5 × 4.6–5.5 μm when fixed and stained. The spore wall has an approximately 43 nm thick, layered exospore with a median layer resembling a double membrane. The polaroplast is uniformly lamellar. The isofilar, 114–123 nm thick, polar filament is arranged in 11–15 (most commonly 12–13) coils in a single layer close to the spore wall. The angle of tilt is approximately 35°. The last coil touches the posterior pole of the spore. A sporophorous vesicle is produced by the sporont. The envelope is generated as a duplication of the plasma membrane. Typically the vesicle primordia grow from one pole of the sporont to the other. The episporontal space initially has granular inclusions. Later septate, persistent tubules of exospore material appear. Simultaneously with the release of sporoblasts, wide tubulus-like, non-persistent structures are formed. They are covered by regularly spaced electron-dense material. Anomalous sporogony was frequently observed, probably caused by a simultaneous parasitism by the fungus Strongwellsea castrans. The systematic position of the microsporidium is discussed, including the reasons for incorporating the genus into the family Glugeidae.

AB - The microsporidium Cystosporogenes deliaradicae sp. no v., a parasite of the adipose tissue of adult cabbage root flies, Delia radicum in Denmark, is described based on light microscopic and ultrastructural characteristics. All life cycle stages have isolated nuclei. The sporogony is polysporoblastic, yielding 4–16 (most commonly 6 or 8) ovocylindrical spores, measuring 1.2–1.5 × 4.6–5.5 μm when fixed and stained. The spore wall has an approximately 43 nm thick, layered exospore with a median layer resembling a double membrane. The polaroplast is uniformly lamellar. The isofilar, 114–123 nm thick, polar filament is arranged in 11–15 (most commonly 12–13) coils in a single layer close to the spore wall. The angle of tilt is approximately 35°. The last coil touches the posterior pole of the spore. A sporophorous vesicle is produced by the sporont. The envelope is generated as a duplication of the plasma membrane. Typically the vesicle primordia grow from one pole of the sporont to the other. The episporontal space initially has granular inclusions. Later septate, persistent tubules of exospore material appear. Simultaneously with the release of sporoblasts, wide tubulus-like, non-persistent structures are formed. They are covered by regularly spaced electron-dense material. Anomalous sporogony was frequently observed, probably caused by a simultaneous parasitism by the fungus Strongwellsea castrans. The systematic position of the microsporidium is discussed, including the reasons for incorporating the genus into the family Glugeidae.

KW - A

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KW - D

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KW - E

KW - endospore

KW - EX

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KW - F

KW - G

KW - Golgi vesicles

KW - HN

KW - host nucleus

KW - Microspora

KW - N

KW - nucleus

KW - P

KW - plasma membrane

KW - PM

KW - polar filament

KW - polar sac

KW - polaroplast

KW - posterior vacuole

KW - PS

KW - PV

KW - S

KW - spore

KW - sporophorous vesicle

KW - T

KW - Taxonomy

KW - tubular inclusion

KW - Ultrastructure

KW - V

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U2 - 10.1016/S0932-4739(11)80091-X

DO - 10.1016/S0932-4739(11)80091-X

M3 - Journal article

AN - SCOPUS:0028854767

SN - 0932-4739

VL - 31

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EP - 285

JO - European Journal of Protistology

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ER -

Ultrastructural study and description of Cystosporogenes deliaradicae n. sp. (microspora, glugeidae), a microsporidian parasite of the cabbage root fly Delia radicum (Linnaeus, 1758) (diptera, anthomyidae)

Abstract

Keywords

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