True microbiota involved in chronic lung infection of cystic fibrosis patients found by culturing and 16S rRNA gene analysis

Vibeke Børsholt Rudkjøbing; Trine R Thomsen; Morten Alhede; Kasper N Kragh; Per H Nielsen; Ulla Johansen; Michael Givskov; Niels Høiby; Thomas Bjarnsholt

doi:10.1128/JCM.06092-11

True microbiota involved in chronic lung infection of cystic fibrosis patients found by culturing and 16S rRNA gene analysis

Vibeke Børsholt Rudkjøbing, Trine R Thomsen, Morten Alhede, Kasper N Kragh, Per H Nielsen, Ulla Johansen, Michael Givskov, Niels Høiby, Thomas Bjarnsholt

Department of Immunology and Microbiology

28 Citations (Scopus)

Abstract

Patients suffering from cystic fibrosis (CF) develop chronic lung infection. In this study, we investigated the microorganisms present in transplanted CF lungs (n = 5) by standard culturing and 16S rRNA gene analysis. A correspondence between culturing and the molecular methods was observed. In conclusion, standard culturing seems reliable for the identification of the dominating pathogens.

Original language	English
Journal	Journal of Clinical Microbiology
Volume	49
Issue number	12
Pages (from-to)	4352-5
Number of pages	4
ISSN	0095-1137
DOIs	https://doi.org/10.1128/JCM.06092-11
Publication status	Published - Dec 2011

Keywords

Bacteria
Biodiversity
Bronchopneumonia
Chronic Disease
Cluster Analysis
Cystic Fibrosis
DNA, Bacterial
DNA, Ribosomal
Humans
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S
Sequence Analysis, DNA

Access to Document

10.1128/JCM.06092-11

Cite this

@article{652068a4306d4483b2095838a6e87966,

title = "True microbiota involved in chronic lung infection of cystic fibrosis patients found by culturing and 16S rRNA gene analysis",

abstract = "Patients suffering from cystic fibrosis (CF) develop chronic lung infection. In this study, we investigated the microorganisms present in transplanted CF lungs (n = 5) by standard culturing and 16S rRNA gene analysis. A correspondence between culturing and the molecular methods was observed. In conclusion, standard culturing seems reliable for the identification of the dominating pathogens.",

keywords = "Bacteria, Biodiversity, Bronchopneumonia, Chronic Disease, Cluster Analysis, Cystic Fibrosis, DNA, Bacterial, DNA, Ribosomal, Humans, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S, Sequence Analysis, DNA",

author = "Rudkj{\o}bing, {Vibeke B{\o}rsholt} and Thomsen, {Trine R} and Morten Alhede and Kragh, {Kasper N} and Nielsen, {Per H} and Ulla Johansen and Michael Givskov and Niels H{\o}iby and Thomas Bjarnsholt",

year = "2011",

month = dec,

doi = "10.1128/JCM.06092-11",

language = "English",

volume = "49",

pages = "4352--5",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "12",

}

TY - JOUR

T1 - True microbiota involved in chronic lung infection of cystic fibrosis patients found by culturing and 16S rRNA gene analysis

AU - Rudkjøbing, Vibeke Børsholt

AU - Thomsen, Trine R

AU - Alhede, Morten

AU - Kragh, Kasper N

AU - Nielsen, Per H

AU - Johansen, Ulla

AU - Givskov, Michael

AU - Høiby, Niels

AU - Bjarnsholt, Thomas

PY - 2011/12

Y1 - 2011/12

N2 - Patients suffering from cystic fibrosis (CF) develop chronic lung infection. In this study, we investigated the microorganisms present in transplanted CF lungs (n = 5) by standard culturing and 16S rRNA gene analysis. A correspondence between culturing and the molecular methods was observed. In conclusion, standard culturing seems reliable for the identification of the dominating pathogens.

AB - Patients suffering from cystic fibrosis (CF) develop chronic lung infection. In this study, we investigated the microorganisms present in transplanted CF lungs (n = 5) by standard culturing and 16S rRNA gene analysis. A correspondence between culturing and the molecular methods was observed. In conclusion, standard culturing seems reliable for the identification of the dominating pathogens.

KW - Bacteria

KW - Biodiversity

KW - Bronchopneumonia

KW - Chronic Disease

KW - Cluster Analysis

KW - Cystic Fibrosis

KW - DNA, Bacterial

KW - DNA, Ribosomal

KW - Humans

KW - Molecular Sequence Data

KW - Phylogeny

KW - RNA, Ribosomal, 16S

KW - Sequence Analysis, DNA

U2 - 10.1128/JCM.06092-11

DO - 10.1128/JCM.06092-11

M3 - Journal article

C2 - 22012018

SN - 0095-1137

VL - 49

SP - 4352

EP - 4355

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 12

ER -

True microbiota involved in chronic lung infection of cystic fibrosis patients found by culturing and 16S rRNA gene analysis

Abstract

Keywords

Access to Document

Fingerprint

Cite this