TY - JOUR
T1 - Transgenic mice with astrocyte-targeted production of interleukin-6 are resistant to high-fat diet-induced increases in body weight and body fat
AU - Hidalgo, Juan
AU - Florit, Sergi
AU - Giralt, Mercedes
AU - Ferrer, Beatriz
AU - Keller, Charlotte
AU - Pilegaard, Henriette
N1 - Keywords: Adiposity; Animals; Astrocytes; Body Composition; Body Weight; Cold Temperature; Diet; Dietary Fats; Female; Glial Fibrillary Acidic Protein; Glucose Tolerance Test; Hypothalamus; Insulin; Interleukin-6; Male; Mice; Mice, Inbred C57BL; Mice, Transgenic; Promoter Regions, Genetic; RNA; Reverse Transcriptase Polymerase Chain Reaction; Sex Characteristics; Stress, Psychological
PY - 2010/1
Y1 - 2010/1
N2 - Interleukin-6 (IL-6) is a major cytokine involved in both normal physiological brain functions and underlying significant neuropathology. IL-6 has been suggested to play a role in the control of body weight but the results are somewhat controversial. In this study we have challenged transgenic mice with astrocyte-targeted IL-6 expression (GFAP-IL6 mice) with a high-fat diet (55% kcal from fat) versus a control diet (10%). The results demonstrate that the GFAP-IL6 mice are resistant to high-fat diet-induced increases in body weight and body fat, apparently without altering food intake and with no evidences of increased sympathetic tone. The high-fat diet-induced impaired responses to an insulin tolerance test (ITT), and to an oral glucose tolerance test (OGTT) in both genotypes. The GFAP-IL6 mice did not differ from littermate wild-type (WT) mice in ITT, but they were more glucose intolerant following the high-fat diet feeding. In summary, the present results demonstrate that brain-specific IL-6 controls body weight which may be a significant factor in physiological conditions and/or in diseases causing neuroinflammation.
AB - Interleukin-6 (IL-6) is a major cytokine involved in both normal physiological brain functions and underlying significant neuropathology. IL-6 has been suggested to play a role in the control of body weight but the results are somewhat controversial. In this study we have challenged transgenic mice with astrocyte-targeted IL-6 expression (GFAP-IL6 mice) with a high-fat diet (55% kcal from fat) versus a control diet (10%). The results demonstrate that the GFAP-IL6 mice are resistant to high-fat diet-induced increases in body weight and body fat, apparently without altering food intake and with no evidences of increased sympathetic tone. The high-fat diet-induced impaired responses to an insulin tolerance test (ITT), and to an oral glucose tolerance test (OGTT) in both genotypes. The GFAP-IL6 mice did not differ from littermate wild-type (WT) mice in ITT, but they were more glucose intolerant following the high-fat diet feeding. In summary, the present results demonstrate that brain-specific IL-6 controls body weight which may be a significant factor in physiological conditions and/or in diseases causing neuroinflammation.
U2 - 10.1016/j.bbi.2009.09.002
DO - 10.1016/j.bbi.2009.09.002
M3 - Journal article
C2 - 19748567
SN - 0889-1591
VL - 24
SP - 119
EP - 126
JO - Brain, Behavior, and Immunity
JF - Brain, Behavior, and Immunity
IS - 1
ER -