Transfection of living HeLa cells with fluorescent poly-cytosine encapsulated  Ag nanoclusters

Yasuko Antoku; Jun-ichi Hotta; Hideaki Mizuno; Robert M. Dickson; Johan Hofkens; Tom Vosch

doi:10.1039/c0pp00015a

Transfection of living HeLa cells with fluorescent poly-cytosine encapsulated Ag nanoclusters

Yasuko Antoku, Jun-ichi Hotta, Hideaki Mizuno, Robert M. Dickson, Johan Hofkens, Tom Vosch

Neuropharm and Genetics

85 Citations (Scopus)

Abstract

The fluorescence of silver clusters encapsulated by single stranded oligo-DNA (24 cytosine base pairs, C₂₄:Ag_n) was used to monitor the transfection of this new silver/DNA fluorophore inside living HeLa cells. For this, the C₂₄:Ag_n molecules were complexed with a commercially available transfection reagent Lipofectamine and the internalization of C₂₄:Ag_n was followed with confocal fluorescence microscopy. Bright near-infrared fluorescence was observed from inside the transfected HeLa cells, when exciting with 633 nm excitation, opening up the possibility for the use of these C₂₄:Ag_n clusters for biological labelling and imaging of living cells and for monitoring the transfection process with limited harm to the living cells.

Original language	English
Journal	Photochemical & Photobiological Sciences
Volume	9
Issue number	5
Pages (from-to)	716-721
Number of pages	6
ISSN	1474-905X
DOIs	https://doi.org/10.1039/c0pp00015a
Publication status	Published - 2010

Access to Document

10.1039/c0pp00015a

http://pubs.rsc.org/en/Content/ArticleLanding/2010/PP/c0pp00015a

Cite this

@article{f0074314a47447f4802a958f299c68cf,

title = "Transfection of living HeLa cells with fluorescent poly-cytosine encapsulated Ag nanoclusters",

abstract = "The fluorescence of silver clusters encapsulated by single stranded oligo-DNA (24 cytosine base pairs, C24:Agn) was used to monitor the transfection of this new silver/DNA fluorophore inside living HeLa cells. For this, the C24:Agn molecules were complexed with a commercially available transfection reagent Lipofectamine and the internalization of C24:Agn was followed with confocal fluorescence microscopy. Bright near-infrared fluorescence was observed from inside the transfected HeLa cells, when exciting with 633 nm excitation, opening up the possibility for the use of these C24:Agn clusters for biological labelling and imaging of living cells and for monitoring the transfection process with limited harm to the living cells.",

author = "Yasuko Antoku and Jun-ichi Hotta and Hideaki Mizuno and Dickson, {Robert M.} and Johan Hofkens and Tom Vosch",

year = "2010",

doi = "10.1039/c0pp00015a",

language = "English",

volume = "9",

pages = "716--721",

journal = "Photochemical and Photobiological Sciences",

issn = "1474-905X",

publisher = "Royal Society of Chemistry",

number = "5",

}

TY - JOUR

T1 - Transfection of living HeLa cells with fluorescent poly-cytosine encapsulated Ag nanoclusters

AU - Antoku, Yasuko

AU - Hotta, Jun-ichi

AU - Mizuno, Hideaki

AU - Dickson, Robert M.

AU - Hofkens, Johan

AU - Vosch, Tom

PY - 2010

Y1 - 2010

N2 - The fluorescence of silver clusters encapsulated by single stranded oligo-DNA (24 cytosine base pairs, C24:Agn) was used to monitor the transfection of this new silver/DNA fluorophore inside living HeLa cells. For this, the C24:Agn molecules were complexed with a commercially available transfection reagent Lipofectamine and the internalization of C24:Agn was followed with confocal fluorescence microscopy. Bright near-infrared fluorescence was observed from inside the transfected HeLa cells, when exciting with 633 nm excitation, opening up the possibility for the use of these C24:Agn clusters for biological labelling and imaging of living cells and for monitoring the transfection process with limited harm to the living cells.

AB - The fluorescence of silver clusters encapsulated by single stranded oligo-DNA (24 cytosine base pairs, C24:Agn) was used to monitor the transfection of this new silver/DNA fluorophore inside living HeLa cells. For this, the C24:Agn molecules were complexed with a commercially available transfection reagent Lipofectamine and the internalization of C24:Agn was followed with confocal fluorescence microscopy. Bright near-infrared fluorescence was observed from inside the transfected HeLa cells, when exciting with 633 nm excitation, opening up the possibility for the use of these C24:Agn clusters for biological labelling and imaging of living cells and for monitoring the transfection process with limited harm to the living cells.

U2 - 10.1039/c0pp00015a

DO - 10.1039/c0pp00015a

M3 - Journal article

C2 - 20442932

SN - 1474-905X

VL - 9

SP - 716

EP - 721

JO - Photochemical and Photobiological Sciences

JF - Photochemical and Photobiological Sciences

IS - 5

ER -

Transfection of living HeLa cells with fluorescent poly-cytosine encapsulated Ag nanoclusters

Abstract

Access to Document

Fingerprint

Cite this