Transcriptional inhibition by the retinoblastoma protein.

A Fattaey; K Helin; E Harlow

doi:10.1098/rstb.1993.0075

Transcriptional inhibition by the retinoblastoma protein.

A Fattaey, K Helin, E Harlow

10 Citations (Scopus)

Abstract

The retinoblastoma protein, pRB, appears to play a key role in coordinating the regulation of cell cycle position and transcriptional events. pRB undergoes specific cell-cycle-dependent phosphorylation, being underphosphorylated in G1 and heavily phosphorylated in S, G2, and M. The underphosphorylated form is able to interact with the E2F transcription factor. Recently, we have cloned a cDNA for E2F-1. By using this clone and a series of non-pRB binding mutants, we have been able to show that the binding of pRB to E2F-1 causes inhibition of E2F-mediated transactivation. pRB's inhibition of E2F-mediated transcription would be lost by mutation in the retinoblastoma gene in human tumours, by pRB's interaction with DNA tumour virus oncoproteins, or by phosphorylation during the cell cycle.

Original language	English
Journal	Philosophical Transactions of the Royal Society of London. Biological Sciences
Volume	340
Issue number	1293
Pages (from-to)	333-6
Number of pages	3
ISSN	0962-8436
DOIs	https://doi.org/10.1098/rstb.1993.0075
Publication status	Published - 1993

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@article{149a12f053e911dd8d9f000ea68e967b,

title = "Transcriptional inhibition by the retinoblastoma protein.",

abstract = "The retinoblastoma protein, pRB, appears to play a key role in coordinating the regulation of cell cycle position and transcriptional events. pRB undergoes specific cell-cycle-dependent phosphorylation, being underphosphorylated in G1 and heavily phosphorylated in S, G2, and M. The underphosphorylated form is able to interact with the E2F transcription factor. Recently, we have cloned a cDNA for E2F-1. By using this clone and a series of non-pRB binding mutants, we have been able to show that the binding of pRB to E2F-1 causes inhibition of E2F-mediated transactivation. pRB's inhibition of E2F-mediated transcription would be lost by mutation in the retinoblastoma gene in human tumours, by pRB's interaction with DNA tumour virus oncoproteins, or by phosphorylation during the cell cycle.",

author = "A Fattaey and K Helin and E Harlow",

note = "Keywords: Adenoviruses, Human; Animals; Carrier Proteins; Cell Cycle; Cell Cycle Proteins; Chloramphenicol O-Acetyltransferase; Cloning, Molecular; DNA; DNA-Binding Proteins; E2F Transcription Factors; E2F1 Transcription Factor; Phosphorylation; Recombinant Fusion Proteins; Retinoblastoma Protein; Trans-Activation (Genetics); Transcription Factor DP1; Transcription Factors; Transcription, Genetic",

year = "1993",

doi = "10.1098/rstb.1993.0075",

language = "English",

volume = "340",

pages = "333--6",

journal = "Philosophical Transactions of the Royal Society B: Biological Sciences",

issn = "0962-8436",

publisher = "The/Royal Society",

number = "1293",

}

TY - JOUR

T1 - Transcriptional inhibition by the retinoblastoma protein.

AU - Fattaey, A

AU - Helin, K

AU - Harlow, E

N1 - Keywords: Adenoviruses, Human; Animals; Carrier Proteins; Cell Cycle; Cell Cycle Proteins; Chloramphenicol O-Acetyltransferase; Cloning, Molecular; DNA; DNA-Binding Proteins; E2F Transcription Factors; E2F1 Transcription Factor; Phosphorylation; Recombinant Fusion Proteins; Retinoblastoma Protein; Trans-Activation (Genetics); Transcription Factor DP1; Transcription Factors; Transcription, Genetic

PY - 1993

Y1 - 1993

N2 - The retinoblastoma protein, pRB, appears to play a key role in coordinating the regulation of cell cycle position and transcriptional events. pRB undergoes specific cell-cycle-dependent phosphorylation, being underphosphorylated in G1 and heavily phosphorylated in S, G2, and M. The underphosphorylated form is able to interact with the E2F transcription factor. Recently, we have cloned a cDNA for E2F-1. By using this clone and a series of non-pRB binding mutants, we have been able to show that the binding of pRB to E2F-1 causes inhibition of E2F-mediated transactivation. pRB's inhibition of E2F-mediated transcription would be lost by mutation in the retinoblastoma gene in human tumours, by pRB's interaction with DNA tumour virus oncoproteins, or by phosphorylation during the cell cycle.

AB - The retinoblastoma protein, pRB, appears to play a key role in coordinating the regulation of cell cycle position and transcriptional events. pRB undergoes specific cell-cycle-dependent phosphorylation, being underphosphorylated in G1 and heavily phosphorylated in S, G2, and M. The underphosphorylated form is able to interact with the E2F transcription factor. Recently, we have cloned a cDNA for E2F-1. By using this clone and a series of non-pRB binding mutants, we have been able to show that the binding of pRB to E2F-1 causes inhibition of E2F-mediated transactivation. pRB's inhibition of E2F-mediated transcription would be lost by mutation in the retinoblastoma gene in human tumours, by pRB's interaction with DNA tumour virus oncoproteins, or by phosphorylation during the cell cycle.

U2 - 10.1098/rstb.1993.0075

DO - 10.1098/rstb.1993.0075

M3 - Journal article

C2 - 8103936

SN - 0962-8436

VL - 340

SP - 333

EP - 336

JO - Philosophical Transactions of the Royal Society B: Biological Sciences

JF - Philosophical Transactions of the Royal Society B: Biological Sciences

IS - 1293

ER -

Transcriptional inhibition by the retinoblastoma protein.

Abstract

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