Toxicity studies of six types of carbon nanoparticles in a chicken-embryo model

Natalia Kurantowicz; Ewa Sawosz; Gabriela Halik; Barbara Strojny; Anna Hotowy; Marta Grodzik; Radoslaw Piast; Wanvimol Pasanphan; André Chwalibog

doi:10.2147/IJN.S131960

Toxicity studies of six types of carbon nanoparticles in a chicken-embryo model

Natalia Kurantowicz, Ewa Sawosz, Gabriela Halik, Barbara Strojny, Anna Hotowy, Marta Grodzik, Radoslaw Piast, Wanvimol Pasanphan, André Chwalibog

15 Citations (Scopus)

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Abstract

In the present study, the toxicity of six different types of carbon nanoparticles (CNPs) was investigated using a chicken-embryo model. Fertilized chicken eggs were divided into the following treatment groups: placebo, diamond NPs, graphite NPs, pristine graphene, small graphene oxide, large graphene oxide, and reduced graphene oxide. Experimental solutions at a concentration of 500 µg/mL were administrated into the egg albumin. Gross pathology and the rate of survival were examined after 5, 10, 15, and 20 days of incubation. After 20 days of incubation, blood samples were collected and the weight of the body and organs measured. The relative ratio of embryo survival decreased after treatment all treatments except diamond NPs. There was no correlation between the rate of survival and the ζ-potential or the surface charge of the CNPs in solution. Body and organ weight, red blood-cell morphology, blood serum biochemical parameters, and oxidative damage in the liver did not differ among the groups. These results indicate that CNPs can remain in blood circulation without any major side effects, suggesting their potential applicability as vehicles for drug delivery or active compounds per se. However, there is a need for further investigation of their properties, which vary depending on production methods and surface functionalization.

Original language	English
Article number	12
Journal	International Journal of Nanomedicine
Volume	2017
Issue number	12
Pages (from-to)	2887-2898
ISSN	1176-9114
DOIs	https://doi.org/10.2147/IJN.S131960
Publication status	Published - 7 Apr 2017

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Toxicity studies of six types of carbon nanoparticles in a chicken-embryo modelFinal published version, 9.5 MBLicence: CC BY-NC

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title = "Toxicity studies of six types of carbon nanoparticles in a chicken-embryo model",

abstract = "In the present study, the toxicity of six different types of carbon nanoparticles (CNPs) was investigated using a chicken-embryo model. Fertilized chicken eggs were divided into the following treatment groups: placebo, diamond NPs, graphite NPs, pristine graphene, small graphene oxide, large graphene oxide, and reduced graphene oxide. Experimental solutions at a concentration of 500 µg/mL were administrated into the egg albumin. Gross pathology and the rate of survival were examined after 5, 10, 15, and 20 days of incubation. After 20 days of incubation, blood samples were collected and the weight of the body and organs measured. The relative ratio of embryo survival decreased after treatment all treatments except diamond NPs. There was no correlation between the rate of survival and the ζ-potential or the surface charge of the CNPs in solution. Body and organ weight, red blood-cell morphology, blood serum biochemical parameters, and oxidative damage in the liver did not differ among the groups. These results indicate that CNPs can remain in blood circulation without any major side effects, suggesting their potential applicability as vehicles for drug delivery or active compounds per se. However, there is a need for further investigation of their properties, which vary depending on production methods and surface functionalization.",

author = "Natalia Kurantowicz and Ewa Sawosz and Gabriela Halik and Barbara Strojny and Anna Hotowy and Marta Grodzik and Radoslaw Piast and Wanvimol Pasanphan and Andr{\'e} Chwalibog",

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T1 - Toxicity studies of six types of carbon nanoparticles in a chicken-embryo model

AU - Kurantowicz, Natalia

AU - Sawosz, Ewa

AU - Halik, Gabriela

AU - Strojny, Barbara

AU - Hotowy, Anna

AU - Grodzik, Marta

AU - Piast, Radoslaw

AU - Pasanphan, Wanvimol

AU - Chwalibog, André

PY - 2017/4/7

Y1 - 2017/4/7

N2 - In the present study, the toxicity of six different types of carbon nanoparticles (CNPs) was investigated using a chicken-embryo model. Fertilized chicken eggs were divided into the following treatment groups: placebo, diamond NPs, graphite NPs, pristine graphene, small graphene oxide, large graphene oxide, and reduced graphene oxide. Experimental solutions at a concentration of 500 µg/mL were administrated into the egg albumin. Gross pathology and the rate of survival were examined after 5, 10, 15, and 20 days of incubation. After 20 days of incubation, blood samples were collected and the weight of the body and organs measured. The relative ratio of embryo survival decreased after treatment all treatments except diamond NPs. There was no correlation between the rate of survival and the ζ-potential or the surface charge of the CNPs in solution. Body and organ weight, red blood-cell morphology, blood serum biochemical parameters, and oxidative damage in the liver did not differ among the groups. These results indicate that CNPs can remain in blood circulation without any major side effects, suggesting their potential applicability as vehicles for drug delivery or active compounds per se. However, there is a need for further investigation of their properties, which vary depending on production methods and surface functionalization.

AB - In the present study, the toxicity of six different types of carbon nanoparticles (CNPs) was investigated using a chicken-embryo model. Fertilized chicken eggs were divided into the following treatment groups: placebo, diamond NPs, graphite NPs, pristine graphene, small graphene oxide, large graphene oxide, and reduced graphene oxide. Experimental solutions at a concentration of 500 µg/mL were administrated into the egg albumin. Gross pathology and the rate of survival were examined after 5, 10, 15, and 20 days of incubation. After 20 days of incubation, blood samples were collected and the weight of the body and organs measured. The relative ratio of embryo survival decreased after treatment all treatments except diamond NPs. There was no correlation between the rate of survival and the ζ-potential or the surface charge of the CNPs in solution. Body and organ weight, red blood-cell morphology, blood serum biochemical parameters, and oxidative damage in the liver did not differ among the groups. These results indicate that CNPs can remain in blood circulation without any major side effects, suggesting their potential applicability as vehicles for drug delivery or active compounds per se. However, there is a need for further investigation of their properties, which vary depending on production methods and surface functionalization.

U2 - 10.2147/IJN.S131960

DO - 10.2147/IJN.S131960

M3 - Journal article

C2 - 28435265

SN - 1176-9114

VL - 2017

SP - 2887

EP - 2898

JO - International Journal of Nanomedicine

JF - International Journal of Nanomedicine

IS - 12

M1 - 12

ER -

Toxicity studies of six types of carbon nanoparticles in a chicken-embryo model

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