Toxicity of silver nanoparticles in monocytes and keratinocytes: potential to induce inflammatory reactions

Piotr Orłowski; Malgorzata Krzyzowska; Anna Winnicka; André Chwalibog; Eva Sawosz

Toxicity of silver nanoparticles in monocytes and keratinocytes: potential to induce inflammatory reactions

Piotr Orłowski, Malgorzata Krzyzowska, Anna Winnicka, André Chwalibog, Eva Sawosz

19 Citations (Scopus)

Abstract

Silver nanoparticles are of interest to be used as antimicrobial agents in wound dressings and coatings in medical devices, but potential adverse effects have been reported in the literature. The possible local inflammatory response to silver nanoparticles and the role of cell death in determining these effects are largely unknown. Effects of the mixture of silver nanoparticles of different sizes were compared in in vitro assays for cytotoxicity, caspase-1 and caspase-9 activity and bax expression. In all tested concentrations, silver nanoparticles were more toxic for RAW 264.7 monocytes than for 291.03C keratinocytes and induced significant caspase-1 activity and necrotic cell death. In keratinocytes, more significantly than in macrophages, silver nanoparticles led to increase of caspase-9 activity and apoptosis. These results indicate that effects of silver nanoparticles depend on the type of exposed cells. In addition, the potency of silver nanoparticles to induce necrosis and caspase-1 activity in monocytes indicates their possible immunotoxic inflammatory potential.

Original language	English
Journal	Central-European Journal of Immunology
Volume	37
Issue number	2
Pages (from-to)	123-130
Number of pages	8
ISSN	1426-3912
Publication status	Published - 2012

Access to Document

Toxicity of silver Orlowski 2012 copyFinal published version, 946 KB

Cite this

@article{22fc13fd2f484ebe9af6f655c2114ea9,

title = "Toxicity of silver nanoparticles in monocytes and keratinocytes: potential to induce inflammatory reactions",

abstract = "Silver nanoparticles are of interest to be used as antimicrobial agents in wound dressings and coatings in medical devices, but potential adverse effects have been reported in the literature. The possible local inflammatory response to silver nanoparticles and the role of cell death in determining these effects are largely unknown. Effects of the mixture of silver nanoparticles of different sizes were compared in in vitro assays for cytotoxicity, caspase-1 and caspase-9 activity and bax expression. In all tested concentrations, silver nanoparticles were more toxic for RAW 264.7 monocytes than for 291.03C keratinocytes and induced significant caspase-1 activity and necrotic cell death. In keratinocytes, more significantly than in macrophages, silver nanoparticles led to increase of caspase-9 activity and apoptosis. These results indicate that effects of silver nanoparticles depend on the type of exposed cells. In addition, the potency of silver nanoparticles to induce necrosis and caspase-1 activity in monocytes indicates their possible immunotoxic inflammatory potential.",

author = "Piotr Or{\l}owski and Malgorzata Krzyzowska and Anna Winnicka and Andr{\'e} Chwalibog and Eva Sawosz",

year = "2012",

language = "English",

volume = "37",

pages = "123--130",

journal = "Central-European Journal of Immunology",

issn = "1426-3912",

publisher = "Termedia sp. z o.o.",

number = "2",

}

TY - JOUR

T1 - Toxicity of silver nanoparticles in monocytes and keratinocytes

T2 - potential to induce inflammatory reactions

AU - Orłowski, Piotr

AU - Krzyzowska, Malgorzata

AU - Winnicka, Anna

AU - Chwalibog, André

AU - Sawosz, Eva

PY - 2012

Y1 - 2012

N2 - Silver nanoparticles are of interest to be used as antimicrobial agents in wound dressings and coatings in medical devices, but potential adverse effects have been reported in the literature. The possible local inflammatory response to silver nanoparticles and the role of cell death in determining these effects are largely unknown. Effects of the mixture of silver nanoparticles of different sizes were compared in in vitro assays for cytotoxicity, caspase-1 and caspase-9 activity and bax expression. In all tested concentrations, silver nanoparticles were more toxic for RAW 264.7 monocytes than for 291.03C keratinocytes and induced significant caspase-1 activity and necrotic cell death. In keratinocytes, more significantly than in macrophages, silver nanoparticles led to increase of caspase-9 activity and apoptosis. These results indicate that effects of silver nanoparticles depend on the type of exposed cells. In addition, the potency of silver nanoparticles to induce necrosis and caspase-1 activity in monocytes indicates their possible immunotoxic inflammatory potential.

AB - Silver nanoparticles are of interest to be used as antimicrobial agents in wound dressings and coatings in medical devices, but potential adverse effects have been reported in the literature. The possible local inflammatory response to silver nanoparticles and the role of cell death in determining these effects are largely unknown. Effects of the mixture of silver nanoparticles of different sizes were compared in in vitro assays for cytotoxicity, caspase-1 and caspase-9 activity and bax expression. In all tested concentrations, silver nanoparticles were more toxic for RAW 264.7 monocytes than for 291.03C keratinocytes and induced significant caspase-1 activity and necrotic cell death. In keratinocytes, more significantly than in macrophages, silver nanoparticles led to increase of caspase-9 activity and apoptosis. These results indicate that effects of silver nanoparticles depend on the type of exposed cells. In addition, the potency of silver nanoparticles to induce necrosis and caspase-1 activity in monocytes indicates their possible immunotoxic inflammatory potential.

UR - http://www.scopus.com/inward/record.url?scp=84862273890&partnerID=8YFLogxK

M3 - Journal article

AN - SCOPUS:84862273890

SN - 1426-3912

VL - 37

SP - 123

EP - 130

JO - Central-European Journal of Immunology

JF - Central-European Journal of Immunology

IS - 2

ER -

Toxicity of silver nanoparticles in monocytes and keratinocytes: potential to induce inflammatory reactions

Abstract

Access to Document

Other files and links

Fingerprint

Cite this