Toxicity of different forms of graphene in a chicken embryo model

Maciej Szmidt; Ewa Sawosz; Kaja Urbanska; Slawomir Jaworski; Marta Kutwin; Anna Hotowy; Mateusz Wierzbicki; Marta Grodzik; Ludwika Lipińska; André Chwalibog

doi:10.1007/s11356-016-7178-z

Toxicity of different forms of graphene in a chicken embryo model

Maciej Szmidt, Ewa Sawosz, Kaja Urbanska, Slawomir Jaworski, Marta Kutwin, Anna Hotowy, Mateusz Wierzbicki, Marta Grodzik, Ludwika Lipińska, André Chwalibog

15 Citations (Scopus)

Abstract

In the present work, the toxicity of three forms of graphene: pristine graphene (pG), graphene oxide (GO), and reduced graphene oxide (rGO) was investigated using a chicken embryo model. Fertilized chicken eggs were divided into the control group and groups administered with pG, GO, and rGO, in concentrations of 50, 500, and 5000 μg/ml. The experimental solutions were injected in ovo into the eggs, and at day 18 of incubation, the embryo survival, body and organ weights, the ultrastructure of liver samples, and the concentration of 8-hydroxy-2′-deoxyguanosine (8-OHdG) in the livers were measured. Survival of embryos decreased significantly after treatment with all types of graphene, but not in a dose-dependent manner. The body weights were only slightly affected by the highest doses of graphene, while the organ weights were not different among treatment groups. In all experimental groups, atypical hepatocyte ultrastructure and mitochondrial damage were observed. The concentration of the marker of DNA damage 8-OHdG in the liver significantly decreased after pG and rGO treatments. Further in vivo studies with different animal models are necessary to clarify the level of toxicity of different types of graphene and to estimate the concentrations appropriate to evaluate their biomedical applications and environmental hazard.

Original language	English
Journal	Environmental Science and Pollution Research
Volume	23
Issue number	19
Pages (from-to)	19940-19948
Number of pages	9
ISSN	0944-1344
DOIs	https://doi.org/10.1007/s11356-016-7178-z
Publication status	Published - 1 Oct 2016

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title = "Toxicity of different forms of graphene in a chicken embryo model",

abstract = "In the present work, the toxicity of three forms of graphene: pristine graphene (pG), graphene oxide (GO), and reduced graphene oxide (rGO) was investigated using a chicken embryo model. Fertilized chicken eggs were divided into the control group and groups administered with pG, GO, and rGO, in concentrations of 50, 500, and 5000 μg/ml. The experimental solutions were injected in ovo into the eggs, and at day 18 of incubation, the embryo survival, body and organ weights, the ultrastructure of liver samples, and the concentration of 8-hydroxy-2′-deoxyguanosine (8-OHdG) in the livers were measured. Survival of embryos decreased significantly after treatment with all types of graphene, but not in a dose-dependent manner. The body weights were only slightly affected by the highest doses of graphene, while the organ weights were not different among treatment groups. In all experimental groups, atypical hepatocyte ultrastructure and mitochondrial damage were observed. The concentration of the marker of DNA damage 8-OHdG in the liver significantly decreased after pG and rGO treatments. Further in vivo studies with different animal models are necessary to clarify the level of toxicity of different types of graphene and to estimate the concentrations appropriate to evaluate their biomedical applications and environmental hazard.",

author = "Maciej Szmidt and Ewa Sawosz and Kaja Urbanska and Slawomir Jaworski and Marta Kutwin and Anna Hotowy and Mateusz Wierzbicki and Marta Grodzik and Ludwika Lipi{\'n}ska and Andr{\'e} Chwalibog",

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T1 - Toxicity of different forms of graphene in a chicken embryo model

AU - Szmidt, Maciej

AU - Sawosz, Ewa

AU - Urbanska, Kaja

AU - Jaworski, Slawomir

AU - Kutwin, Marta

AU - Hotowy, Anna

AU - Wierzbicki, Mateusz

AU - Grodzik, Marta

AU - Lipińska, Ludwika

AU - Chwalibog, André

PY - 2016/10/1

Y1 - 2016/10/1

N2 - In the present work, the toxicity of three forms of graphene: pristine graphene (pG), graphene oxide (GO), and reduced graphene oxide (rGO) was investigated using a chicken embryo model. Fertilized chicken eggs were divided into the control group and groups administered with pG, GO, and rGO, in concentrations of 50, 500, and 5000 μg/ml. The experimental solutions were injected in ovo into the eggs, and at day 18 of incubation, the embryo survival, body and organ weights, the ultrastructure of liver samples, and the concentration of 8-hydroxy-2′-deoxyguanosine (8-OHdG) in the livers were measured. Survival of embryos decreased significantly after treatment with all types of graphene, but not in a dose-dependent manner. The body weights were only slightly affected by the highest doses of graphene, while the organ weights were not different among treatment groups. In all experimental groups, atypical hepatocyte ultrastructure and mitochondrial damage were observed. The concentration of the marker of DNA damage 8-OHdG in the liver significantly decreased after pG and rGO treatments. Further in vivo studies with different animal models are necessary to clarify the level of toxicity of different types of graphene and to estimate the concentrations appropriate to evaluate their biomedical applications and environmental hazard.

AB - In the present work, the toxicity of three forms of graphene: pristine graphene (pG), graphene oxide (GO), and reduced graphene oxide (rGO) was investigated using a chicken embryo model. Fertilized chicken eggs were divided into the control group and groups administered with pG, GO, and rGO, in concentrations of 50, 500, and 5000 μg/ml. The experimental solutions were injected in ovo into the eggs, and at day 18 of incubation, the embryo survival, body and organ weights, the ultrastructure of liver samples, and the concentration of 8-hydroxy-2′-deoxyguanosine (8-OHdG) in the livers were measured. Survival of embryos decreased significantly after treatment with all types of graphene, but not in a dose-dependent manner. The body weights were only slightly affected by the highest doses of graphene, while the organ weights were not different among treatment groups. In all experimental groups, atypical hepatocyte ultrastructure and mitochondrial damage were observed. The concentration of the marker of DNA damage 8-OHdG in the liver significantly decreased after pG and rGO treatments. Further in vivo studies with different animal models are necessary to clarify the level of toxicity of different types of graphene and to estimate the concentrations appropriate to evaluate their biomedical applications and environmental hazard.

U2 - 10.1007/s11356-016-7178-z

DO - 10.1007/s11356-016-7178-z

M3 - Journal article

C2 - 27436378

SN - 0944-1344

VL - 23

SP - 19940

EP - 19948

JO - Environmental Science and Pollution Research

JF - Environmental Science and Pollution Research

IS - 19

ER -

Toxicity of different forms of graphene in a chicken embryo model

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