Tissue sample stability: thawing effect on multi-organ samples

TY - JOUR

T1 - Tissue sample stability: thawing effect on multi-organ samples

AU - Torell, Frida

AU - Bennett, Kate

AU - Cereghini, Silvia

AU - Rannar, Stefan

AU - Lundstedt-Enkel, Katrin

AU - Moritz, Thomas

AU - Haumaitre, Cecile

AU - Trygg, Johan

AU - Lundstedt, Torbjorn

PY - 2016/2/1

Y1 - 2016/2/1

N2 - Correct handling of samples is essential in metabolomic studies. Improper handling and prolonged storage of samples has unwanted effects on the metabolite levels. The aim of this study was to identify the effects that thawing has on different organ samples. Organ samples from gut, kidney, liver, muscle and pancreas were analyzed for a number of endogenous metabolites in an untargeted metabolomics approach, using gas chromatography time of flight mass spectrometry at the Swedish Metabolomics Centre, Umeå University, Sweden. Multivariate data analysis was performed by means of principal component analysis and orthogonal projection to latent structures discriminant analysis. The results showed that the metabolic changes caused by thawing were almost identical for all organs. As expected, there was a marked increase in overall metabolite levels after thawing, caused by increased protein and cell degradation. Cholesterol was one of the eight metabolites found to be decreased in the thawed samples in all organ groups. The results also indicated that the muscles are less susceptible to oxidation compared to the rest of the organ samples.

AB - Correct handling of samples is essential in metabolomic studies. Improper handling and prolonged storage of samples has unwanted effects on the metabolite levels. The aim of this study was to identify the effects that thawing has on different organ samples. Organ samples from gut, kidney, liver, muscle and pancreas were analyzed for a number of endogenous metabolites in an untargeted metabolomics approach, using gas chromatography time of flight mass spectrometry at the Swedish Metabolomics Centre, Umeå University, Sweden. Multivariate data analysis was performed by means of principal component analysis and orthogonal projection to latent structures discriminant analysis. The results showed that the metabolic changes caused by thawing were almost identical for all organs. As expected, there was a marked increase in overall metabolite levels after thawing, caused by increased protein and cell degradation. Cholesterol was one of the eight metabolites found to be decreased in the thawed samples in all organ groups. The results also indicated that the muscles are less susceptible to oxidation compared to the rest of the organ samples.

U2 - 10.1007/s11306-015-0933-1

DO - 10.1007/s11306-015-0933-1

M3 - Journal article

SN - 1573-3882

VL - 12

JO - Metabolomics

JF - Metabolomics

IS - 2

ER -