Abstract
Background: At present plasma tissue inhibitor of metalloproteinases-1 (TIMP-1) is undergoing validation as a biological marker in colorectal cancer (CRC). The clinical implementation of plasma TIMP-1 in prognosis, prediction, screening and monitoring CRC requires robust information as to the influence of preanalytical factors, including inter-and intrapersonal biological variations. The aim of the present study was to evaluate the possible effects of smoking on the level of TIMP-1 in plasma from healthy subjects. Materials and methods: Forty-six never-smokers and 48 daily smokers participated in the 13-week study. Smokers were randomized into 3 groups of 16 subjects each: one group continued to smoke, a second group refrained from smoking and used a transdermal nicotine patch, and a third group refrained from smoking and used placebo patches. Plasma TIMP-1 levels were determined using ELISA. Results: No significant differences in TIMP-1 levels could be shown between the smoking group, the 2 different abstinent smoking groups and the never-smoking group. Conclusion: Smoking does not appear to have any influence on TIMP-1 levels in plasma collected from healthy subjects.
| Original language | English |
|---|---|
| Journal | International Journal of Biological Markers |
| Volume | 28 |
| Issue number | 2 |
| Pages (from-to) | 226-230 |
| Number of pages | 5 |
| ISSN | 0393-6155 |
| DOIs | |
| Publication status | Published - Apr 2013 |
