Time-course investigation of infection with a low virulent Pasteurella multocida strain in normal and immune-suppressed 12-week-old free-range chickens

P.G. Mbuthia; L.W. Njagi; P.N. Nyaga; L.C. Bebora; U. Minga; Jens Peter Christensen; John Elmerdahl Olsen

doi:10.1080/03079457.2011.623298

Time-course investigation of infection with a low virulent Pasteurella multocida strain in normal and immune-suppressed 12-week-old free-range chickens

P.G. Mbuthia, L.W. Njagi, P.N. Nyaga, L.C. Bebora, U. Minga, Jens Peter Christensen, John Elmerdahl Olsen

3 Citations (Scopus)

Abstract

Twelve-week-old indigenous chickens, either immune-suppressed using dexamethasone (IS) or non-immune-suppressed (NIS), were challenged with a low virulent strain, Pasteurella multocida strain NCTC 10322(T), and developed clinical signs and pathological lesions typical of chronic fowl cholera. NIS birds demonstrated much more severe signs of fowl cholera than IS birds. With few exceptions, signs recorded in IS and NIS birds were of the same types, but significantly milder in the IS birds, indicating that immune suppression does not change the course of infection but rather the severity of signs in fowl cholera. P. multocida signals by fluorescent in situ hybridization (FISH) were observed between 1 h and 14 days in the lungs, trachea, air sacs, liver, spleen, bursa of Fabricius and caecal tonsils, while signals from other organs mostly were observed after 24 h. More organs had FISH signals in NIS birds than in IS birds and at higher frequency per organ. Many organs were positive by FISH even 14 days post infection, and it is suggested that these organs may be likely places for long-term carriage of P. multocida following infection. The present study has demonstrated the spread of P. multocida in different tissues in chickens and distribution of lesions associated with chronic fowl cholera, and pointed to a decrease of pathology in IS birds. Since dexamethasone mostly affects heterophils, the study suggests that these cells play a role in the development of lesions associated with chronic fowl cholera in chickens.

Original language	English
Journal	Avian Pathology
Volume	40
Issue number	6
Pages (from-to)	629-637
Number of pages	9
ISSN	0307-9457
DOIs	https://doi.org/10.1080/03079457.2011.623298
Publication status	Published - Dec 2011

Keywords

Analysis of Variance
Animals
Bacterial Load
Chickens
Cholera
Dexamethasone
Histological Techniques
Immunosuppression
In Situ Hybridization, Fluorescence
Pasteurella Infections
Pasteurella multocida
Poultry Diseases
Time Factors
Former LIFE faculty

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{25f4db06bc3649d385b5dee8d82614e4,

title = "Time-course investigation of infection with a low virulent Pasteurella multocida strain in normal and immune-suppressed 12-week-old free-range chickens",

abstract = "Twelve-week-old indigenous chickens, either immune-suppressed using dexamethasone (IS) or non-immune-suppressed (NIS), were challenged with a low virulent strain, Pasteurella multocida strain NCTC 10322(T), and developed clinical signs and pathological lesions typical of chronic fowl cholera. NIS birds demonstrated much more severe signs of fowl cholera than IS birds. With few exceptions, signs recorded in IS and NIS birds were of the same types, but significantly milder in the IS birds, indicating that immune suppression does not change the course of infection but rather the severity of signs in fowl cholera. P. multocida signals by fluorescent in situ hybridization (FISH) were observed between 1 h and 14 days in the lungs, trachea, air sacs, liver, spleen, bursa of Fabricius and caecal tonsils, while signals from other organs mostly were observed after 24 h. More organs had FISH signals in NIS birds than in IS birds and at higher frequency per organ. Many organs were positive by FISH even 14 days post infection, and it is suggested that these organs may be likely places for long-term carriage of P. multocida following infection. The present study has demonstrated the spread of P. multocida in different tissues in chickens and distribution of lesions associated with chronic fowl cholera, and pointed to a decrease of pathology in IS birds. Since dexamethasone mostly affects heterophils, the study suggests that these cells play a role in the development of lesions associated with chronic fowl cholera in chickens.",

keywords = "Analysis of Variance, Animals, Bacterial Load, Chickens, Cholera, Dexamethasone, Histological Techniques, Immunosuppression, In Situ Hybridization, Fluorescence, Pasteurella Infections, Pasteurella multocida, Poultry Diseases, Time Factors, Former LIFE faculty, Pasteurella , multocida, chicken, immune supression",

author = "P.G. Mbuthia and L.W. Njagi and P.N. Nyaga and L.C. Bebora and U. Minga and Christensen, {Jens Peter} and Olsen, {John Elmerdahl}",

year = "2011",

month = dec,

doi = "10.1080/03079457.2011.623298",

language = "English",

volume = "40",

pages = "629--637",

journal = "Avian Pathology",

issn = "0307-9457",

publisher = "Taylor & Francis",

number = "6",

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TY - JOUR

T1 - Time-course investigation of infection with a low virulent Pasteurella multocida strain in normal and immune-suppressed 12-week-old free-range chickens

AU - Mbuthia, P.G.

AU - Njagi, L.W.

AU - Nyaga, P.N.

AU - Bebora, L.C.

AU - Minga, U.

AU - Christensen, Jens Peter

AU - Olsen, John Elmerdahl

PY - 2011/12

Y1 - 2011/12

N2 - Twelve-week-old indigenous chickens, either immune-suppressed using dexamethasone (IS) or non-immune-suppressed (NIS), were challenged with a low virulent strain, Pasteurella multocida strain NCTC 10322(T), and developed clinical signs and pathological lesions typical of chronic fowl cholera. NIS birds demonstrated much more severe signs of fowl cholera than IS birds. With few exceptions, signs recorded in IS and NIS birds were of the same types, but significantly milder in the IS birds, indicating that immune suppression does not change the course of infection but rather the severity of signs in fowl cholera. P. multocida signals by fluorescent in situ hybridization (FISH) were observed between 1 h and 14 days in the lungs, trachea, air sacs, liver, spleen, bursa of Fabricius and caecal tonsils, while signals from other organs mostly were observed after 24 h. More organs had FISH signals in NIS birds than in IS birds and at higher frequency per organ. Many organs were positive by FISH even 14 days post infection, and it is suggested that these organs may be likely places for long-term carriage of P. multocida following infection. The present study has demonstrated the spread of P. multocida in different tissues in chickens and distribution of lesions associated with chronic fowl cholera, and pointed to a decrease of pathology in IS birds. Since dexamethasone mostly affects heterophils, the study suggests that these cells play a role in the development of lesions associated with chronic fowl cholera in chickens.

AB - Twelve-week-old indigenous chickens, either immune-suppressed using dexamethasone (IS) or non-immune-suppressed (NIS), were challenged with a low virulent strain, Pasteurella multocida strain NCTC 10322(T), and developed clinical signs and pathological lesions typical of chronic fowl cholera. NIS birds demonstrated much more severe signs of fowl cholera than IS birds. With few exceptions, signs recorded in IS and NIS birds were of the same types, but significantly milder in the IS birds, indicating that immune suppression does not change the course of infection but rather the severity of signs in fowl cholera. P. multocida signals by fluorescent in situ hybridization (FISH) were observed between 1 h and 14 days in the lungs, trachea, air sacs, liver, spleen, bursa of Fabricius and caecal tonsils, while signals from other organs mostly were observed after 24 h. More organs had FISH signals in NIS birds than in IS birds and at higher frequency per organ. Many organs were positive by FISH even 14 days post infection, and it is suggested that these organs may be likely places for long-term carriage of P. multocida following infection. The present study has demonstrated the spread of P. multocida in different tissues in chickens and distribution of lesions associated with chronic fowl cholera, and pointed to a decrease of pathology in IS birds. Since dexamethasone mostly affects heterophils, the study suggests that these cells play a role in the development of lesions associated with chronic fowl cholera in chickens.

KW - Analysis of Variance

KW - Animals

KW - Bacterial Load

KW - Chickens

KW - Cholera

KW - Dexamethasone

KW - Histological Techniques

KW - Immunosuppression

KW - In Situ Hybridization, Fluorescence

KW - Pasteurella Infections

KW - Pasteurella multocida

KW - Poultry Diseases

KW - Time Factors

KW - Former LIFE faculty

KW - Pasteurella

KW - multocida

KW - chicken

KW - immune supression

U2 - 10.1080/03079457.2011.623298

DO - 10.1080/03079457.2011.623298

M3 - Journal article

C2 - 22107097

SN - 0307-9457

VL - 40

SP - 629

EP - 637

JO - Avian Pathology

JF - Avian Pathology

IS - 6

ER -

Time-course investigation of infection with a low virulent Pasteurella multocida strain in normal and immune-suppressed 12-week-old free-range chickens

Abstract

Keywords

UN SDGs

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