The wheat ABC transporter Lr34 modifies the lipid environment at the plasma membrane

Johannes P. Deppe; Ritta Rabbat; Stefan Hörtensteiner; Beat Keller; Enrico Martinoia; Rosa L. Lopéz-Marqués

doi:10.1074/jbc.ra118.002532

The wheat ABC transporter Lr34 modifies the lipid environment at the plasma membrane

Johannes P. Deppe^*, Ritta Rabbat, Stefan Hörtensteiner, Beat Keller, Enrico Martinoia, Rosa L. Lopéz-Marqués

^*Corresponding author for this work

Section for Transport Biology

5 Citations (Scopus)

Abstract

Phospholipids (PLs) are emerging as important factors that initiate signal transduction cascades at the plasma membrane. Their distribution within biological membranes is tightly regulated, e.g. by ATP-binding cassette (ABC) transporters, which preferably translocate PLs from the cytoplasmic to the exoplasmic membrane leaflet and are therefore called PL-floppases. Here, we demonstrate that a plant ABC transporter, Lr34 from wheat (Triticum aestivum), is involved in plasma membrane remodeling characterized by an intracellular accumulation of phosphatidic acid and enhanced outward translocation of phosphatidylserine. In addition, the content of phosphatidylinositol 4,5-bisphosphate in the cytoplasmic leaflet of the plasma membrane was reduced in the presence of the ABC transporter. When heterologously expressed in Saccharomyces cerevisiae, Lr34 promoted oil body formation in a mutant defective in PL-transfer in the secretory pathway. Our results suggest that PL redistribution by Lr34 potentially affects the membrane-bound proteome and contributes to the previously reported stimuli-independent activation of biotic and abiotic stress responses and neutral lipid accumulation in transgenic Lr34-expressing barley plants.

Original language	English
Journal	Journal of Biological Chemistry
Volume	293
Issue number	48
Pages (from-to)	18667-18679
Number of pages	13
ISSN	0021-9258
DOIs	https://doi.org/10.1074/jbc.ra118.002532
Publication status	Published - 30 Nov 2018

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title = "The wheat ABC transporter Lr34 modifies the lipid environment at the plasma membrane",

abstract = "Phospholipids (PLs) are emerging as important factors that initiate signal transduction cascades at the plasma membrane. Their distribution within biological membranes is tightly regulated, e.g. by ATP-binding cassette (ABC) transporters, which preferably translocate PLs from the cytoplasmic to the exoplasmic membrane leaflet and are therefore called PL-floppases. Here, we demonstrate that a plant ABC transporter, Lr34 from wheat (Triticum aestivum), is involved in plasma membrane remodeling characterized by an intracellular accumulation of phosphatidic acid and enhanced outward translocation of phosphatidylserine. In addition, the content of phosphatidylinositol 4,5-bisphosphate in the cytoplasmic leaflet of the plasma membrane was reduced in the presence of the ABC transporter. When heterologously expressed in Saccharomyces cerevisiae, Lr34 promoted oil body formation in a mutant defective in PL-transfer in the secretory pathway. Our results suggest that PL redistribution by Lr34 potentially affects the membrane-bound proteome and contributes to the previously reported stimuli-independent activation of biotic and abiotic stress responses and neutral lipid accumulation in transgenic Lr34-expressing barley plants.",

author = "Deppe, {Johannes P.} and Ritta Rabbat and Stefan H{\"o}rtensteiner and Beat Keller and Enrico Martinoia and Lop{\'e}z-Marqu{\'e}s, {Rosa L.}",

year = "2018",

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doi = "10.1074/jbc.ra118.002532",

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T1 - The wheat ABC transporter Lr34 modifies the lipid environment at the plasma membrane

AU - Deppe, Johannes P.

AU - Rabbat, Ritta

AU - Hörtensteiner, Stefan

AU - Keller, Beat

AU - Martinoia, Enrico

AU - Lopéz-Marqués, Rosa L.

PY - 2018/11/30

Y1 - 2018/11/30

N2 - Phospholipids (PLs) are emerging as important factors that initiate signal transduction cascades at the plasma membrane. Their distribution within biological membranes is tightly regulated, e.g. by ATP-binding cassette (ABC) transporters, which preferably translocate PLs from the cytoplasmic to the exoplasmic membrane leaflet and are therefore called PL-floppases. Here, we demonstrate that a plant ABC transporter, Lr34 from wheat (Triticum aestivum), is involved in plasma membrane remodeling characterized by an intracellular accumulation of phosphatidic acid and enhanced outward translocation of phosphatidylserine. In addition, the content of phosphatidylinositol 4,5-bisphosphate in the cytoplasmic leaflet of the plasma membrane was reduced in the presence of the ABC transporter. When heterologously expressed in Saccharomyces cerevisiae, Lr34 promoted oil body formation in a mutant defective in PL-transfer in the secretory pathway. Our results suggest that PL redistribution by Lr34 potentially affects the membrane-bound proteome and contributes to the previously reported stimuli-independent activation of biotic and abiotic stress responses and neutral lipid accumulation in transgenic Lr34-expressing barley plants.

AB - Phospholipids (PLs) are emerging as important factors that initiate signal transduction cascades at the plasma membrane. Their distribution within biological membranes is tightly regulated, e.g. by ATP-binding cassette (ABC) transporters, which preferably translocate PLs from the cytoplasmic to the exoplasmic membrane leaflet and are therefore called PL-floppases. Here, we demonstrate that a plant ABC transporter, Lr34 from wheat (Triticum aestivum), is involved in plasma membrane remodeling characterized by an intracellular accumulation of phosphatidic acid and enhanced outward translocation of phosphatidylserine. In addition, the content of phosphatidylinositol 4,5-bisphosphate in the cytoplasmic leaflet of the plasma membrane was reduced in the presence of the ABC transporter. When heterologously expressed in Saccharomyces cerevisiae, Lr34 promoted oil body formation in a mutant defective in PL-transfer in the secretory pathway. Our results suggest that PL redistribution by Lr34 potentially affects the membrane-bound proteome and contributes to the previously reported stimuli-independent activation of biotic and abiotic stress responses and neutral lipid accumulation in transgenic Lr34-expressing barley plants.

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DO - 10.1074/jbc.ra118.002532

M3 - Journal article

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AN - SCOPUS:85057551335

SN - 0021-9258

VL - 293

SP - 18667

EP - 18679

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 48

ER -

The wheat ABC transporter Lr34 modifies the lipid environment at the plasma membrane

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