Abstract
The majority of all genes have so far been identified and annotated systematically through in silico gene finding. Here we report the finding of 3662 strand-specific transcriptionally active regions (TARs) in the genome of Bacillus subtilis by the use of tiling arrays. We have measured the genome-wide expression during mid-exponential growth on rich (LB) and minimal (M9) medium. The identified TARs account for 77.3% of the genes as they are currently annotated and additionally we find 84 putative non-coding RNAs (ncRNAs) and 127 antisense transcripts. One ncRNA, ncr22, is predicted to act as a translational control on cstA and an antisense transcript was observed opposite the housekeeping sigma factor sigA. Through this work we have discovered a long conserved 3' untranslated region (UTR) in a group of membrane-associated genes that is predicted to fold into a large and highly stable secondary structure. One of the genes having this tail is efeN, which encodes a target of the twin-arginine translocase (Tat) protein translocation system.
| Original language | English |
|---|---|
| Journal | Molecular Microbiology |
| Volume | 73 |
| Issue number | 6 |
| Pages (from-to) | 1043-57 |
| Number of pages | 15 |
| ISSN | 0950-382X |
| DOIs | |
| Publication status | Published - Sept 2009 |
| Externally published | Yes |
Keywords
- Bacillus subtilis/genetics
- Base Sequence
- Culture Media/chemistry
- Gene Expression Profiling/methods
- Models, Molecular
- Molecular Sequence Data
- Nucleic Acid Conformation
- RNA, Antisense/biosynthesis
- RNA, Untranslated/biosynthesis
- Transcription, Genetic