The Scottish Structural Proteomics Facility: targets, methods and outputs

Muse Oke; Lester G Carter; Kenneth A Johnson; Huanting Liu; Stephen A McMahon; Xuan Yan; Melina Kerou; Nadine D Weikart; Nadia Kadi; Md Arif Sheikh; Stefan Schmelz; Mark Dorward; Michal Zawadzki; Christopher Cozens; Helen Falconer; Helen Powers; Ian M Overton; C A Johannes van Niekerk; Xu Peng; Prakash Patel; Roger Antony Garrett; David Prangishvili; Catherine H Botting; Peter J Coote; David T F Dryden; Geoffrey J Barton; Ulrich Schwarz-Linek; Gregory L Challis; Garry L Taylor; Malcolm F White; James H Naismith

doi:10.1007/s10969-010-9090-y

The Scottish Structural Proteomics Facility: targets, methods and outputs

Muse Oke, Lester G Carter, Kenneth A Johnson, Huanting Liu, Stephen A McMahon, Xuan Yan, Melina Kerou, Nadine D Weikart, Nadia Kadi, Md Arif Sheikh, Stefan Schmelz, Mark Dorward, Michal Zawadzki, Christopher Cozens, Helen Falconer, Helen Powers, Ian M Overton, C A Johannes van Niekerk, Xu Peng, Prakash PatelRoger Antony Garrett, David Prangishvili, Catherine H Botting, Peter J Coote, David T F Dryden, Geoffrey J Barton, Ulrich Schwarz-Linek, Gregory L Challis, Garry L Taylor, Malcolm F White, James H Naismith

Functional Genomics

83 Citations (Scopus)

Abstract

The Scottish Structural Proteomics Facility was funded to develop a laboratory scale approach to high throughput structure determination. The effort was successful in that over 40 structures were determined. These structures and the methods harnessed to obtain them are reported here. This report reflects on the value of automation but also on the continued requirement for a high degree of scientific and technical expertise. The efficiency of the process poses challenges to the current paradigm of structural analysis and publication. In the 5 year period we published ten peer-reviewed papers reporting structural data arising from the pipeline. Nevertheless, the number of structures solved exceeded our ability to analyse and publish each new finding. By reporting the experimental details and depositing the structures we hope to maximize the impact of the project by allowing others to follow up the relevant biology.

Original language	English
Journal	Journal of Structural and Functional Genomics
Volume	11
Issue number	2
Pages (from-to)	167-80
Number of pages	14
ISSN	1345-711X
DOIs	https://doi.org/10.1007/s10969-010-9090-y
Publication status	Published - 1 Jun 2010

Keywords

Computational Biology
Crystallization
Humans
Laboratories
Proteins
Proteomics
Scotland

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Oke, M., Carter, L. G., Johnson, K. A., Liu, H., McMahon, S. A., Yan, X., Kerou, M., Weikart, N. D., Kadi, N., Sheikh, M. A., Schmelz, S., Dorward, M., Zawadzki, M., Cozens, C., Falconer, H., Powers, H., Overton, I. M., van Niekerk, C. A. J., Peng, X., ... Naismith, J. H. (2010). The Scottish Structural Proteomics Facility: targets, methods and outputs. Journal of Structural and Functional Genomics, 11(2), 167-80. https://doi.org/10.1007/s10969-010-9090-y

Oke, M, Carter, LG, Johnson, KA, Liu, H, McMahon, SA, Yan, X, Kerou, M, Weikart, ND, Kadi, N, Sheikh, MA, Schmelz, S, Dorward, M, Zawadzki, M, Cozens, C, Falconer, H, Powers, H, Overton, IM, van Niekerk, CAJ, Peng, X, Patel, P, Garrett, RA, Prangishvili, D, Botting, CH, Coote, PJ, Dryden, DTF, Barton, GJ, Schwarz-Linek, U, Challis, GL, Taylor, GL, White, MF & Naismith, JH 2010, 'The Scottish Structural Proteomics Facility: targets, methods and outputs', Journal of Structural and Functional Genomics, vol. 11, no. 2, pp. 167-80. https://doi.org/10.1007/s10969-010-9090-y

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AU - Patel, Prakash

AU - Garrett, Roger Antony

AU - Prangishvili, David

AU - Botting, Catherine H

AU - Coote, Peter J

AU - Dryden, David T F

AU - Barton, Geoffrey J

AU - Schwarz-Linek, Ulrich

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AB - The Scottish Structural Proteomics Facility was funded to develop a laboratory scale approach to high throughput structure determination. The effort was successful in that over 40 structures were determined. These structures and the methods harnessed to obtain them are reported here. This report reflects on the value of automation but also on the continued requirement for a high degree of scientific and technical expertise. The efficiency of the process poses challenges to the current paradigm of structural analysis and publication. In the 5 year period we published ten peer-reviewed papers reporting structural data arising from the pipeline. Nevertheless, the number of structures solved exceeded our ability to analyse and publish each new finding. By reporting the experimental details and depositing the structures we hope to maximize the impact of the project by allowing others to follow up the relevant biology.

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KW - Crystallization

KW - Humans

KW - Laboratories

KW - Proteins

KW - Proteomics

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JF - Journal of Structural and Functional Genomics

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ER -

The Scottish Structural Proteomics Facility: targets, methods and outputs

Abstract

Keywords

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