Abstract
1,25-Dihydroxyvitamin D3 (1,25-(OH)2D3) inhibits immunoglobulin production by human mononuclear cells (MNC) in vitro. The present study was undertaken to evaluate the role of T cells and monocytes in 1,25-(OH)2D3 induced suppression of B cell functions. The synthetic vitamin D3 analogue MC 903 was examined in parallel. 1,25-(OH)2D3 and MC 903 showed a dose-related inhibition of IgM, IgG and IgA plaque-forming cells in poke-weed mitogen (PWM) activated cultures of MNC. This effect was most likely mediated through impairment of T cell and monocyte functions. First, the inhibitory effect was seen after PWM stimulation, but not after Epstein-Barr virus stimulation which activates B cells independently of T cells and monocytes. Second, 1,25-(OH)2D3 was not effective in T cell and monocyte-depleted cultures. Third, the effect of 1,25-(OH)2D3 on PWM driven MNC was reversed by addition of the recombinant monokines: interleukin (IL)-1 beta, tumour necrosis factor alpha (rTNF alpha), rIL-6, as well as the lymphokines: lymphotoxin (rLT) and rIL-2. This is consistent with the finding that 1,25-(OH)2D3 also inhibited IL-1 alpha, TNF alpha and LT production in these cultures. The assumption that B cells are not directly affected by 1,25-(OH)2D3 was further supported by the fact that 24 h of culture with 10(-8) M 1,25-(OH)2D3 failed to reduce immunoglobulin production by in vivo activated B cells.
| Original language | English |
|---|---|
| Journal | Immunopharmacology |
| Volume | 21 |
| Issue number | 2 |
| Pages (from-to) | 121-8 |
| Number of pages | 8 |
| ISSN | 0162-3109 |
| Publication status | Published - 1 Mar 1991 |
Keywords
- Antibody Formation
- B-Lymphocytes
- Calcitriol
- Cell Communication
- Humans
- In Vitro Techniques
- Interleukin-1
- Lymphocyte Activation
- Monocytes
- T-Lymphocytes
- Tumor Necrosis Factor-alpha
- Journal Article
- Research Support, Non-U.S. Gov't