The product of the ABC half-transporter gene ABCG2 (BCRP/MXR/ABCP) is expressed in the plasma membrane

TY - JOUR

T1 - The product of the ABC half-transporter gene ABCG2 (BCRP/MXR/ABCP) is expressed in the plasma membrane

AU - Rocchi, E

AU - Khodjakov, A

AU - Volk, E L

AU - Yang, C H

AU - Litman, Thomas

AU - Bates, S E

AU - Schneider, E

N1 - Copyright 2000 Academic Press.

PY - 2000/4/29

Y1 - 2000/4/29

N2 - The products of the ABC gene family can be generally classified as either full-transporters of half-transporters. Full-transporters are expressed in the plasma membrane, whereas half-transporters are usually found in intracellular membranes. Recently, an ABC half-transporter, the ABCG2 gene product Breast Cancer/Mitoxantrone Resistance Protein (BCRP/MXR), has been shown to cause mitoxantrone and topotecan resistance. The purpose of this study was to determine the expression and the intracellular localization of this protein in various drug-resistant cell lines. BCRP/MXR expression was determined by Western blot and immunohistochemistry. This protein is highly overexpressed in several drug-resistant cell lines and localizes predominantly to the plasma membrane, instead of to intracellular membranes as seen with all other known half-transporters. Therefore, BCRP/MXR is unique among the ABC half-transporters by being localized to the plasma membrane.

AB - The products of the ABC gene family can be generally classified as either full-transporters of half-transporters. Full-transporters are expressed in the plasma membrane, whereas half-transporters are usually found in intracellular membranes. Recently, an ABC half-transporter, the ABCG2 gene product Breast Cancer/Mitoxantrone Resistance Protein (BCRP/MXR), has been shown to cause mitoxantrone and topotecan resistance. The purpose of this study was to determine the expression and the intracellular localization of this protein in various drug-resistant cell lines. BCRP/MXR expression was determined by Western blot and immunohistochemistry. This protein is highly overexpressed in several drug-resistant cell lines and localizes predominantly to the plasma membrane, instead of to intracellular membranes as seen with all other known half-transporters. Therefore, BCRP/MXR is unique among the ABC half-transporters by being localized to the plasma membrane.

KW - ATP-Binding Cassette Transporters

KW - Blotting, Western

KW - Breast Neoplasms

KW - Cell Membrane

KW - Colonic Neoplasms

KW - Drug Resistance, Neoplasm

KW - Humans

KW - Immunohistochemistry

KW - Microscopy, Fluorescence

KW - Tumor Cells, Cultured

U2 - 10.1006/bbrc.2000.2590

DO - 10.1006/bbrc.2000.2590

M3 - Journal article

C2 - 10777678

SN - 0006-291X

VL - 271

SP - 42

EP - 46

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -