The polymerase chain reaction can reveal the occurrence of naturally mixed infections with Leishmania parasites

TY - JOUR

T1 - The polymerase chain reaction can reveal the occurrence of naturally mixed infections with Leishmania parasites

AU - Ibrahim, M E

AU - Smyth, A J

AU - Ali, M H

AU - Barker, D C

AU - Kharazmi, A

N1 - Keywords: Animals; Base Sequence; DNA, Protozoan; Electrophoresis, Starch Gel; Humans; Leishmania; Leishmania donovani; Leishmania major; Leishmaniasis; Molecular Sequence Data; Polymerase Chain Reaction

PY - 1994

Y1 - 1994

N2 - On isolation and characterization of Leishmania parasites from Sudanese patients with visceral leishmaniasis (VL), four cases of mixed infections were found. Three of those cases were from the Eastern Sudan focus of VL. In one case the patient was found to be concomitantly infected with Leishmania donovani and Leishmania aethiopica, while the remaining three patients possessed mixed infections of Leishmania donovani and Leishmania major. Mixed infections were identified by PCR amplification of Leishmania kinetoplast DNA (kDNA) from parasites in culture or in original patient aspirate material and, additionally in the former cases by isoenzyme electrophoresis. In those cases where parasite culture was successful, PCR also demonstrated the rapidity with which one Leishmania species was eliminated from culture during continuous passage.

AB - On isolation and characterization of Leishmania parasites from Sudanese patients with visceral leishmaniasis (VL), four cases of mixed infections were found. Three of those cases were from the Eastern Sudan focus of VL. In one case the patient was found to be concomitantly infected with Leishmania donovani and Leishmania aethiopica, while the remaining three patients possessed mixed infections of Leishmania donovani and Leishmania major. Mixed infections were identified by PCR amplification of Leishmania kinetoplast DNA (kDNA) from parasites in culture or in original patient aspirate material and, additionally in the former cases by isoenzyme electrophoresis. In those cases where parasite culture was successful, PCR also demonstrated the rapidity with which one Leishmania species was eliminated from culture during continuous passage.

M3 - Journal article

C2 - 7810388

SN - 0001-706X

VL - 57

SP - 327

EP - 332

JO - Acta Tropica

JF - Acta Tropica

IS - 4

ER -