The phagocytic fitness of leucopatches may impact the healing of chronic wounds

K Thomsen; H Trøstrup; L. Christophersen; R Lundquist; N Høiby; C Moser

doi:10.1111/cei.12773

The phagocytic fitness of leucopatches may impact the healing of chronic wounds

K Thomsen, H Trøstrup, L. Christophersen, R Lundquist, N Høiby, C Moser

12 Citations (Scopus)

Abstract

Chronic non-healing wounds are significantly bothersome to patients and can result in severe complications. In addition, they are increasing in numbers, and a challenging problem to the health-care system. Handling of chronic, non-healing wounds can be discouraging due to lack of improvement, and a recent explanation can be the involvement of biofilm infections in the pathogenesis of non-healing wounds. Therefore, new treatment alternatives to improve outcome are continuously sought-after. Autologous leucopatches are such a new, adjunctive treatment option, showing promising clinical effects. However, the beneficial effect of the patches are not understood fully, although a major contribution is believed to be from the release of stimulating growth factors from activated thrombocytes within the leucopatch. Because the leucopatches also contain substantial numbers of leucocytes, the aim of the present study was to investigate the activity of the polymorphonuclear neutrophils (PMNs) within the leucopatch. By means of burst assay, phagocytosis assay, migration assay, biofilm killing assay and fluorescence in-situ hybridization (FISH) assay we showed significant respiratory burst in PMNs, active phagocytosis and killing of Pseudomonas aeruginosa by the leucopatch. In addition, bacterial-induced migration of PMNs from the leucopatch was shown, as well as uptake of P. aeruginosa by PMNs within the leucopatch. The present study substantiated that at least part of the beneficial clinical effect in chronic wounds by leucopatches is attributed to the activity of the PMNs in the leucopatch.

Original language	English
Journal	Clinical and Experimental Immunology
Volume	184
Issue number	3
Pages (from-to)	368-77
Number of pages	10
ISSN	0009-9104
DOIs	https://doi.org/10.1111/cei.12773
Publication status	Published - 1 Jun 2016

Keywords

Adult
Biofilms
Cell Movement
Female
Healthy Volunteers
Humans
Immunologic Factors
In Situ Hybridization
Male
Middle Aged
Models, Biological
Neutrophils
Phagocytosis
Platelet-Rich Plasma
Primary Cell Culture
Pseudomonas aeruginosa
Reactive Oxygen Species
Respiratory Burst
Wound Healing
Journal Article

Access to Document

10.1111/cei.12773

cei.12773Final published version, 574 KB

Cite this

@article{0dd0550d0e6f4f64a34a331e124eff07,

title = "The phagocytic fitness of leucopatches may impact the healing of chronic wounds",

abstract = "Chronic non-healing wounds are significantly bothersome to patients and can result in severe complications. In addition, they are increasing in numbers, and a challenging problem to the health-care system. Handling of chronic, non-healing wounds can be discouraging due to lack of improvement, and a recent explanation can be the involvement of biofilm infections in the pathogenesis of non-healing wounds. Therefore, new treatment alternatives to improve outcome are continuously sought-after. Autologous leucopatches are such a new, adjunctive treatment option, showing promising clinical effects. However, the beneficial effect of the patches are not understood fully, although a major contribution is believed to be from the release of stimulating growth factors from activated thrombocytes within the leucopatch. Because the leucopatches also contain substantial numbers of leucocytes, the aim of the present study was to investigate the activity of the polymorphonuclear neutrophils (PMNs) within the leucopatch. By means of burst assay, phagocytosis assay, migration assay, biofilm killing assay and fluorescence in-situ hybridization (FISH) assay we showed significant respiratory burst in PMNs, active phagocytosis and killing of Pseudomonas aeruginosa by the leucopatch. In addition, bacterial-induced migration of PMNs from the leucopatch was shown, as well as uptake of P. aeruginosa by PMNs within the leucopatch. The present study substantiated that at least part of the beneficial clinical effect in chronic wounds by leucopatches is attributed to the activity of the PMNs in the leucopatch.",

keywords = "Adult, Biofilms, Cell Movement, Female, Healthy Volunteers, Humans, Immunologic Factors, In Situ Hybridization, Male, Middle Aged, Models, Biological, Neutrophils, Phagocytosis, Platelet-Rich Plasma, Primary Cell Culture, Pseudomonas aeruginosa, Reactive Oxygen Species, Respiratory Burst, Wound Healing, Journal Article",

author = "K Thomsen and H Tr{\o}strup and L. Christophersen and R Lundquist and N H{\o}iby and C Moser",

note = "{\textcopyright} 2016 British Society for Immunology.",

year = "2016",

month = jun,

day = "1",

doi = "10.1111/cei.12773",

language = "English",

volume = "184",

pages = "368--77",

journal = "Clinical and Experimental Immunology",

issn = "0009-9104",

publisher = "Wiley-Blackwell",

number = "3",

}

TY - JOUR

T1 - The phagocytic fitness of leucopatches may impact the healing of chronic wounds

AU - Thomsen, K

AU - Trøstrup, H

AU - Christophersen, L.

AU - Lundquist, R

AU - Høiby, N

AU - Moser, C

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Chronic non-healing wounds are significantly bothersome to patients and can result in severe complications. In addition, they are increasing in numbers, and a challenging problem to the health-care system. Handling of chronic, non-healing wounds can be discouraging due to lack of improvement, and a recent explanation can be the involvement of biofilm infections in the pathogenesis of non-healing wounds. Therefore, new treatment alternatives to improve outcome are continuously sought-after. Autologous leucopatches are such a new, adjunctive treatment option, showing promising clinical effects. However, the beneficial effect of the patches are not understood fully, although a major contribution is believed to be from the release of stimulating growth factors from activated thrombocytes within the leucopatch. Because the leucopatches also contain substantial numbers of leucocytes, the aim of the present study was to investigate the activity of the polymorphonuclear neutrophils (PMNs) within the leucopatch. By means of burst assay, phagocytosis assay, migration assay, biofilm killing assay and fluorescence in-situ hybridization (FISH) assay we showed significant respiratory burst in PMNs, active phagocytosis and killing of Pseudomonas aeruginosa by the leucopatch. In addition, bacterial-induced migration of PMNs from the leucopatch was shown, as well as uptake of P. aeruginosa by PMNs within the leucopatch. The present study substantiated that at least part of the beneficial clinical effect in chronic wounds by leucopatches is attributed to the activity of the PMNs in the leucopatch.

AB - Chronic non-healing wounds are significantly bothersome to patients and can result in severe complications. In addition, they are increasing in numbers, and a challenging problem to the health-care system. Handling of chronic, non-healing wounds can be discouraging due to lack of improvement, and a recent explanation can be the involvement of biofilm infections in the pathogenesis of non-healing wounds. Therefore, new treatment alternatives to improve outcome are continuously sought-after. Autologous leucopatches are such a new, adjunctive treatment option, showing promising clinical effects. However, the beneficial effect of the patches are not understood fully, although a major contribution is believed to be from the release of stimulating growth factors from activated thrombocytes within the leucopatch. Because the leucopatches also contain substantial numbers of leucocytes, the aim of the present study was to investigate the activity of the polymorphonuclear neutrophils (PMNs) within the leucopatch. By means of burst assay, phagocytosis assay, migration assay, biofilm killing assay and fluorescence in-situ hybridization (FISH) assay we showed significant respiratory burst in PMNs, active phagocytosis and killing of Pseudomonas aeruginosa by the leucopatch. In addition, bacterial-induced migration of PMNs from the leucopatch was shown, as well as uptake of P. aeruginosa by PMNs within the leucopatch. The present study substantiated that at least part of the beneficial clinical effect in chronic wounds by leucopatches is attributed to the activity of the PMNs in the leucopatch.

KW - Adult

KW - Biofilms

KW - Cell Movement

KW - Female

KW - Healthy Volunteers

KW - Humans

KW - Immunologic Factors

KW - In Situ Hybridization

KW - Male

KW - Middle Aged

KW - Models, Biological

KW - Neutrophils

KW - Phagocytosis

KW - Platelet-Rich Plasma

KW - Primary Cell Culture

KW - Pseudomonas aeruginosa

KW - Reactive Oxygen Species

KW - Respiratory Burst

KW - Wound Healing

KW - Journal Article

U2 - 10.1111/cei.12773

DO - 10.1111/cei.12773

M3 - Journal article

C2 - 26830371

SN - 0009-9104

VL - 184

SP - 368

EP - 377

JO - Clinical and Experimental Immunology

JF - Clinical and Experimental Immunology

IS - 3

ER -

The phagocytic fitness of leucopatches may impact the healing of chronic wounds

Abstract

Keywords

Access to Document

Fingerprint

Cite this