The origin and function of platelet glycosyltransferases

Hans H Wandall; Viktoria Rumjantseva; Anne Louise Tølbøll Sørensen; Sunita Patel-Hett; Emma C Josefsson; Eric P Bennett; Joseph E Italiano; Henrik Clausen; John H Hartwig; Karin M Hoffmeister

doi:10.1182/blood-2012-02-409235

The origin and function of platelet glycosyltransferases

Hans H Wandall, Viktoria Rumjantseva, Anne Louise Tølbøll Sørensen, Sunita Patel-Hett, Emma C Josefsson, Eric P Bennett, Joseph E Italiano, Henrik Clausen, John H Hartwig, Karin M Hoffmeister

54 Citations (Scopus)

Abstract

Platelets are megakaryocyte subfragments that participate in hemostatic and host defense reactions and deliver pro-and antiangiogenic factors throughout the vascular system. Although they are anucleated cells that lack a complex secretory apparatus with distinct Golgi/ endoplasmic reticulum compartments, past studies have shown that platelets have glycosyltransferase activities. In the present study, we show that members of 3 distinct glycosyltransferase families are found within and on the surface of platelets. Immunocytology and flow cytometry results indicated that megakaryocytes package these Golgi-derived glycosyltransferases into vesicles that are sent via proplatelets to nascent platelets, where they accumulate. These glycosyltransferases are active, and intact platelets glycosylate large exogenous substrates. Furthermore, we show that activation of platelets results in the release of soluble glycosyltransferase activities and that platelets contain sufficient levels of sugar nucleotides for detection of glycosylation of exogenously added substrates. Therefore, the results of the present study show that blood platelets are a rich source of both glycosyltransferases and donor sugar substrates that can be released to function in the extracellular space. This platelet-glycosylation machinery offers a pathway to a simple glycoengineering strategy improving storage of platelets and may serve hitherto unknown biologic functions.

Original language	English
Journal	Blood
Volume	120
Issue number	3
Pages (from-to)	626-635
Number of pages	10
ISSN	0006-4971
DOIs	https://doi.org/10.1182/blood-2012-02-409235
Publication status	Published - 19 Jul 2012

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title = "The origin and function of platelet glycosyltransferases",

abstract = "Platelets are megakaryocyte subfragments that participate in hemostatic and host defense reactions and deliver pro-and antiangiogenic factors throughout the vascular system. Although they are anucleated cells that lack a complex secretory apparatus with distinct Golgi/ endoplasmic reticulum compartments, past studies have shown that platelets have glycosyltransferase activities. In the present study, we show that members of 3 distinct glycosyltransferase families are found within and on the surface of platelets. Immunocytology and flow cytometry results indicated that megakaryocytes package these Golgi-derived glycosyltransferases into vesicles that are sent via proplatelets to nascent platelets, where they accumulate. These glycosyltransferases are active, and intact platelets glycosylate large exogenous substrates. Furthermore, we show that activation of platelets results in the release of soluble glycosyltransferase activities and that platelets contain sufficient levels of sugar nucleotides for detection of glycosylation of exogenously added substrates. Therefore, the results of the present study show that blood platelets are a rich source of both glycosyltransferases and donor sugar substrates that can be released to function in the extracellular space. This platelet-glycosylation machinery offers a pathway to a simple glycoengineering strategy improving storage of platelets and may serve hitherto unknown biologic functions.",

author = "Wandall, {Hans H} and Viktoria Rumjantseva and S{\o}rensen, {Anne Louise T{\o}lb{\o}ll} and Sunita Patel-Hett and Josefsson, {Emma C} and Bennett, {Eric P} and Italiano, {Joseph E} and Henrik Clausen and Hartwig, {John H} and Hoffmeister, {Karin M}",

year = "2012",

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doi = "10.1182/blood-2012-02-409235",

language = "English",

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journal = "Blood",

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T1 - The origin and function of platelet glycosyltransferases

AU - Wandall, Hans H

AU - Rumjantseva, Viktoria

AU - Sørensen, Anne Louise Tølbøll

AU - Patel-Hett, Sunita

AU - Josefsson, Emma C

AU - Bennett, Eric P

AU - Italiano, Joseph E

AU - Clausen, Henrik

AU - Hartwig, John H

AU - Hoffmeister, Karin M

PY - 2012/7/19

Y1 - 2012/7/19

N2 - Platelets are megakaryocyte subfragments that participate in hemostatic and host defense reactions and deliver pro-and antiangiogenic factors throughout the vascular system. Although they are anucleated cells that lack a complex secretory apparatus with distinct Golgi/ endoplasmic reticulum compartments, past studies have shown that platelets have glycosyltransferase activities. In the present study, we show that members of 3 distinct glycosyltransferase families are found within and on the surface of platelets. Immunocytology and flow cytometry results indicated that megakaryocytes package these Golgi-derived glycosyltransferases into vesicles that are sent via proplatelets to nascent platelets, where they accumulate. These glycosyltransferases are active, and intact platelets glycosylate large exogenous substrates. Furthermore, we show that activation of platelets results in the release of soluble glycosyltransferase activities and that platelets contain sufficient levels of sugar nucleotides for detection of glycosylation of exogenously added substrates. Therefore, the results of the present study show that blood platelets are a rich source of both glycosyltransferases and donor sugar substrates that can be released to function in the extracellular space. This platelet-glycosylation machinery offers a pathway to a simple glycoengineering strategy improving storage of platelets and may serve hitherto unknown biologic functions.

AB - Platelets are megakaryocyte subfragments that participate in hemostatic and host defense reactions and deliver pro-and antiangiogenic factors throughout the vascular system. Although they are anucleated cells that lack a complex secretory apparatus with distinct Golgi/ endoplasmic reticulum compartments, past studies have shown that platelets have glycosyltransferase activities. In the present study, we show that members of 3 distinct glycosyltransferase families are found within and on the surface of platelets. Immunocytology and flow cytometry results indicated that megakaryocytes package these Golgi-derived glycosyltransferases into vesicles that are sent via proplatelets to nascent platelets, where they accumulate. These glycosyltransferases are active, and intact platelets glycosylate large exogenous substrates. Furthermore, we show that activation of platelets results in the release of soluble glycosyltransferase activities and that platelets contain sufficient levels of sugar nucleotides for detection of glycosylation of exogenously added substrates. Therefore, the results of the present study show that blood platelets are a rich source of both glycosyltransferases and donor sugar substrates that can be released to function in the extracellular space. This platelet-glycosylation machinery offers a pathway to a simple glycoengineering strategy improving storage of platelets and may serve hitherto unknown biologic functions.

U2 - 10.1182/blood-2012-02-409235

DO - 10.1182/blood-2012-02-409235

M3 - Journal article

C2 - 22613794

SN - 0006-4971

VL - 120

SP - 626

EP - 635

JO - Blood

JF - Blood

IS - 3

ER -

The origin and function of platelet glycosyltransferases

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