The motogenic and mitogenic responses to HGF are amplified by the Shc adaptor protein.

TY - JOUR

T1 - The motogenic and mitogenic responses to HGF are amplified by the Shc adaptor protein.

AU - Pelicci, G

AU - Giordano, S

AU - Zhen, Z

AU - Salcini, A E

AU - Lanfrancone, L

AU - Bardelli, A

AU - Panayotou, G

AU - Waterfield, M D

AU - Ponzetto, C

AU - Pelicci, P G

N1 - Keywords: Adaptor Proteins, Signal Transducing; Amino Acid Sequence; Animals; Cell Division; Cell Movement; GRB2 Adaptor Protein; Haplorhini; Hepatocyte Growth Factor; Humans; Mice; Molecular Sequence Data; Phosphorylation; Proteins; Proto-Oncogene Proteins c-met; Receptor Protein-Tyrosine Kinases

PY - 1995

Y1 - 1995

N2 - The receptor of Hepatocyte Growth Factor-Scatter Factor (HGF) is a tyrosine kinase which regulates cell motility and growth. After ligand-induced tyrosine phosphorylation, the HGF receptor associates with the Shc adaptor, via the SH2 domain. Site-directed mutagenesis of the HGF receptor indicates that phosphotyrosines Y1349VHV and Y1356VNV can work as docking sites for Shc. The Kd of this interaction, measured in real time using synthetic phosphopeptides and recombinant Shc on a BIAcore biosensor, is 150 nm for both sites. After stimulation of the HGF receptor, Shc is phosphorylated on Y317VNV, generating an high affinity binding site for Grb2 (Kd = 15 nM). This duplicates the high affinity binding site for Grb2 present on the HGF receptor (Y1356VNV). Thus HGF stimulation can trigger the Ras pathway by recruiting Grb2 both directly through the receptor, and indirectly, through Shc. Overexpression of wild-type Shc, but not of the Y317-->F mutant, enhances cell migration and growth in response to HGF. These data show that Shc is a relevant substrate of the HGF receptor, and works as an 'amplifier' of the motogenic as well as of the mitogenic response.

AB - The receptor of Hepatocyte Growth Factor-Scatter Factor (HGF) is a tyrosine kinase which regulates cell motility and growth. After ligand-induced tyrosine phosphorylation, the HGF receptor associates with the Shc adaptor, via the SH2 domain. Site-directed mutagenesis of the HGF receptor indicates that phosphotyrosines Y1349VHV and Y1356VNV can work as docking sites for Shc. The Kd of this interaction, measured in real time using synthetic phosphopeptides and recombinant Shc on a BIAcore biosensor, is 150 nm for both sites. After stimulation of the HGF receptor, Shc is phosphorylated on Y317VNV, generating an high affinity binding site for Grb2 (Kd = 15 nM). This duplicates the high affinity binding site for Grb2 present on the HGF receptor (Y1356VNV). Thus HGF stimulation can trigger the Ras pathway by recruiting Grb2 both directly through the receptor, and indirectly, through Shc. Overexpression of wild-type Shc, but not of the Y317-->F mutant, enhances cell migration and growth in response to HGF. These data show that Shc is a relevant substrate of the HGF receptor, and works as an 'amplifier' of the motogenic as well as of the mitogenic response.

M3 - Journal article

C2 - 7731718

SN - 0950-9232

VL - 10

SP - 1631

EP - 1638

JO - Oncogene

JF - Oncogene

IS - 8

ER -