The microbiota continuum along the female reproductive tract and its relation to uterine-related diseases

Chen Chen; Xiaolei Song; Weixia Wei; Huanzi Zhong; Juanjuan Dai; Zhou Lan; Fei Li; Xinlei Yu; Qiang Feng; Zirong Wang; Hailiang Xie; Xiaomin Chen; Chunwei Zeng; Bo Wen; Liping Zeng; Hui Du; Huiru Tang; Changlu Xu; Yan Xia; Huihua Xia; Huanming Yang; Jian Wang; Jun Wang; Lise Madsen; Susanne Brix; Karsten Kristiansen; Xun Xu; Junhua Li; Ruifang Wu; Huijue Jia

doi:10.1038/s41467-017-00901-0

The microbiota continuum along the female reproductive tract and its relation to uterine-related diseases

Chen Chen, Xiaolei Song, Weixia Wei, Huanzi Zhong, Juanjuan Dai, Zhou Lan, Fei Li, Xinlei Yu, Qiang Feng, Zirong Wang, Hailiang Xie, Xiaomin Chen, Chunwei Zeng, Bo Wen, Liping Zeng, Hui Du, Huiru Tang, Changlu Xu, Yan Xia, Huihua XiaHuanming Yang, Jian Wang, Jun Wang, Lise Madsen, Susanne Brix, Karsten Kristiansen, Xun Xu, Junhua Li^*, Ruifang Wu, Huijue Jia

^*Corresponding author for this work

Genome Research and Molecular Bio Medicine

187 Citations (Scopus)

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Abstract

Reports on bacteria detected in maternal fluids during pregnancy are typically associated with adverse consequences, and whether the female reproductive tract harbours distinct microbial communities beyond the vagina has been a matter of debate. Here we systematically sample the microbiota within the female reproductive tract in 110 women of reproductive age, and examine the nature of colonisation by 16S rRNA gene amplicon sequencing and cultivation. We find distinct microbial communities in cervical canal, uterus, fallopian tubes and peritoneal fluid, differing from that of the vagina. The results reflect a microbiota continuum along the female reproductive tract, indicative of a non-sterile environment. We also identify microbial taxa and potential functions that correlate with the menstrual cycle or are over-represented in subjects with adenomyosis or infertility due to endometriosis. The study provides insight into the nature of the vagino-uterine microbiome, and suggests that surveying the vaginal or cervical microbiota might be useful for detection of common diseases in the upper reproductive tract.

Original language	English
Article number	875
Journal	Nature Communications
Volume	8
Issue number	1
Number of pages	11
ISSN	2041-1723
DOIs	https://doi.org/10.1038/s41467-017-00901-0
Publication status	Published - 17 Oct 2017

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Chen_2017_The_microbiota_continuumFinal published version, 2.69 MBLicence: CC BY

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Chen, C., Song, X., Wei, W., Zhong, H., Dai, J., Lan, Z., Li, F., Yu, X., Feng, Q., Wang, Z., Xie, H., Chen, X., Zeng, C., Wen, B., Zeng, L., Du, H., Tang, H., Xu, C., Xia, Y., ... Jia, H. (2017). The microbiota continuum along the female reproductive tract and its relation to uterine-related diseases. Nature Communications, 8(1), [875]. https://doi.org/10.1038/s41467-017-00901-0

Chen, C, Song, X, Wei, W, Zhong, H, Dai, J, Lan, Z, Li, F, Yu, X, Feng, Q, Wang, Z, Xie, H, Chen, X, Zeng, C, Wen, B, Zeng, L, Du, H, Tang, H, Xu, C, Xia, Y, Xia, H, Yang, H, Wang, J, Wang, J, Madsen, L, Brix, S, Kristiansen, K, Xu, X, Li, J, Wu, R & Jia, H 2017, 'The microbiota continuum along the female reproductive tract and its relation to uterine-related diseases', Nature Communications, vol. 8, no. 1, 875. https://doi.org/10.1038/s41467-017-00901-0

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abstract = "Reports on bacteria detected in maternal fluids during pregnancy are typically associated with adverse consequences, and whether the female reproductive tract harbours distinct microbial communities beyond the vagina has been a matter of debate. Here we systematically sample the microbiota within the female reproductive tract in 110 women of reproductive age, and examine the nature of colonisation by 16S rRNA gene amplicon sequencing and cultivation. We find distinct microbial communities in cervical canal, uterus, fallopian tubes and peritoneal fluid, differing from that of the vagina. The results reflect a microbiota continuum along the female reproductive tract, indicative of a non-sterile environment. We also identify microbial taxa and potential functions that correlate with the menstrual cycle or are over-represented in subjects with adenomyosis or infertility due to endometriosis. The study provides insight into the nature of the vagino-uterine microbiome, and suggests that surveying the vaginal or cervical microbiota might be useful for detection of common diseases in the upper reproductive tract.",

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AU - Li, Fei

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AU - Xu, Changlu

AU - Xia, Yan

AU - Xia, Huihua

AU - Yang, Huanming

AU - Wang, Jian

AU - Wang, Jun

AU - Madsen, Lise

AU - Brix, Susanne

AU - Kristiansen, Karsten

AU - Xu, Xun

AU - Li, Junhua

AU - Wu, Ruifang

AU - Jia, Huijue

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The microbiota continuum along the female reproductive tract and its relation to uterine-related diseases

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