The membrane transporter PotE is required for virulence in avian pathogenic Escherichia coli (APEC)

Priscila Regina Guerra; Ana Herrero-Fresno; Susanne Elisabeth Pors; Shahana Ahmed; Dan Wang; Ida Thøfner; Fabio Antenucci; John Elmerdahl Olsen

doi:10.1016/j.vetmic.2018.01.011

The membrane transporter PotE is required for virulence in avian pathogenic Escherichia coli (APEC)

Priscila Regina Guerra, Ana Herrero-Fresno, Susanne Elisabeth Pors, Shahana Ahmed, Dan Wang, Ida Thøfner, Fabio Antenucci, John Elmerdahl Olsen^*

^*Corresponding author for this work

3 Citations (Scopus)

Abstract

Over the last few years, polyamines have been described as key-signal of virulence in pathogenic bacteria. In the current study, we investigated whether the knockout of genes related to polyamine biosynthesis and putrescine transport affected the virulence of an avian pathogenic E. coli (APEC) strain. One-week-old White Leghorn chickens were infected intratracheally with mutants in polyamine biosynthesis (ΔspeB/C and ΔspeD/E) and transport genes (ΔpotE) of a well-characterized APEC strain of ST117 (O83: H4). All polyamine mutants and the wild-type strain were able to infect chicken; however, we observed significantly fewer lesions in the lungs of the chickens infected with the polyamine mutants in comparison with chicken infected with the wild-type. Results derived from histology of infected lungs detected significantly fewer lesions in the lung of birds infected within particular the putrescine transport mutant (ΔpotE). A decrease in colonization levels was observed in the liver and spleen of birds infected with the putrescine biosynthesis mutant ΔspeB/C, and likewise, a decrease of the colonization levels of all organs from birds infected with the ΔpotE was detected. Together, our data demonstrate that the deletion of polyamine genes, and in particular the PotE membrane protein, attenuates the virulence of APEC during infection of chickens.

Original language	English
Journal	Veterinary Microbiology
Volume	216
Pages (from-to)	38-44
Number of pages	7
ISSN	0378-1135
DOIs	https://doi.org/10.1016/j.vetmic.2018.01.011
Publication status	Published - Mar 2018

Keywords

Chicken
E. coli
Embryo
Histopathology
Putrescine
Spermidine

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10.1016/j.vetmic.2018.01.011

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@article{b1e42c18fa4946e2af22abc9472f3acf,

title = "The membrane transporter PotE is required for virulence in avian pathogenic Escherichia coli (APEC)",

abstract = "Over the last few years, polyamines have been described as key-signal of virulence in pathogenic bacteria. In the current study, we investigated whether the knockout of genes related to polyamine biosynthesis and putrescine transport affected the virulence of an avian pathogenic E. coli (APEC) strain. One-week-old White Leghorn chickens were infected intratracheally with mutants in polyamine biosynthesis (ΔspeB/C and ΔspeD/E) and transport genes (ΔpotE) of a well-characterized APEC strain of ST117 (O83: H4). All polyamine mutants and the wild-type strain were able to infect chicken; however, we observed significantly fewer lesions in the lungs of the chickens infected with the polyamine mutants in comparison with chicken infected with the wild-type. Results derived from histology of infected lungs detected significantly fewer lesions in the lung of birds infected within particular the putrescine transport mutant (ΔpotE). A decrease in colonization levels was observed in the liver and spleen of birds infected with the putrescine biosynthesis mutant ΔspeB/C, and likewise, a decrease of the colonization levels of all organs from birds infected with the ΔpotE was detected. Together, our data demonstrate that the deletion of polyamine genes, and in particular the PotE membrane protein, attenuates the virulence of APEC during infection of chickens.",

keywords = "Chicken, E. coli, Embryo, Histopathology, Putrescine, Spermidine",

author = "Guerra, {Priscila Regina} and Ana Herrero-Fresno and Pors, {Susanne Elisabeth} and Shahana Ahmed and Dan Wang and Ida Th{\o}fner and Fabio Antenucci and Olsen, {John Elmerdahl}",

year = "2018",

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doi = "10.1016/j.vetmic.2018.01.011",

language = "English",

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pages = "38--44",

journal = "Veterinary Microbiology",

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T1 - The membrane transporter PotE is required for virulence in avian pathogenic Escherichia coli (APEC)

AU - Guerra, Priscila Regina

AU - Herrero-Fresno, Ana

AU - Pors, Susanne Elisabeth

AU - Ahmed, Shahana

AU - Wang, Dan

AU - Thøfner, Ida

AU - Antenucci, Fabio

AU - Olsen, John Elmerdahl

PY - 2018/3

Y1 - 2018/3

N2 - Over the last few years, polyamines have been described as key-signal of virulence in pathogenic bacteria. In the current study, we investigated whether the knockout of genes related to polyamine biosynthesis and putrescine transport affected the virulence of an avian pathogenic E. coli (APEC) strain. One-week-old White Leghorn chickens were infected intratracheally with mutants in polyamine biosynthesis (ΔspeB/C and ΔspeD/E) and transport genes (ΔpotE) of a well-characterized APEC strain of ST117 (O83: H4). All polyamine mutants and the wild-type strain were able to infect chicken; however, we observed significantly fewer lesions in the lungs of the chickens infected with the polyamine mutants in comparison with chicken infected with the wild-type. Results derived from histology of infected lungs detected significantly fewer lesions in the lung of birds infected within particular the putrescine transport mutant (ΔpotE). A decrease in colonization levels was observed in the liver and spleen of birds infected with the putrescine biosynthesis mutant ΔspeB/C, and likewise, a decrease of the colonization levels of all organs from birds infected with the ΔpotE was detected. Together, our data demonstrate that the deletion of polyamine genes, and in particular the PotE membrane protein, attenuates the virulence of APEC during infection of chickens.

AB - Over the last few years, polyamines have been described as key-signal of virulence in pathogenic bacteria. In the current study, we investigated whether the knockout of genes related to polyamine biosynthesis and putrescine transport affected the virulence of an avian pathogenic E. coli (APEC) strain. One-week-old White Leghorn chickens were infected intratracheally with mutants in polyamine biosynthesis (ΔspeB/C and ΔspeD/E) and transport genes (ΔpotE) of a well-characterized APEC strain of ST117 (O83: H4). All polyamine mutants and the wild-type strain were able to infect chicken; however, we observed significantly fewer lesions in the lungs of the chickens infected with the polyamine mutants in comparison with chicken infected with the wild-type. Results derived from histology of infected lungs detected significantly fewer lesions in the lung of birds infected within particular the putrescine transport mutant (ΔpotE). A decrease in colonization levels was observed in the liver and spleen of birds infected with the putrescine biosynthesis mutant ΔspeB/C, and likewise, a decrease of the colonization levels of all organs from birds infected with the ΔpotE was detected. Together, our data demonstrate that the deletion of polyamine genes, and in particular the PotE membrane protein, attenuates the virulence of APEC during infection of chickens.

KW - Chicken

KW - E. coli

KW - Embryo

KW - Histopathology

KW - Putrescine

KW - Spermidine

U2 - 10.1016/j.vetmic.2018.01.011

DO - 10.1016/j.vetmic.2018.01.011

M3 - Journal article

C2 - 29519523

AN - SCOPUS:85041604476

SN - 0378-1135

VL - 216

SP - 38

EP - 44

JO - Veterinary Microbiology

JF - Veterinary Microbiology

ER -

The membrane transporter PotE is required for virulence in avian pathogenic Escherichia coli (APEC)

Abstract

Keywords

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