The MAP kinase substrate MKS1 is a regulator of plant defense responses.

Erik Andreasson; Thomas Jenkins; Peter Brodersen; Stephan Thorgrimsen; Nikolaj H T Petersen; Shijiang Zhu; Jin-Long Qiu; Pernille Micheelsen; Anne Rocher; Morten Petersen; Mari-Anne Newman; Henrik Bjørn Nielsen; Heribert Hirt; Imre Somssich; Ole Mattsson; John Mundy

doi:10.1038/sj.emboj.7600737

The MAP kinase substrate MKS1 is a regulator of plant defense responses.

Erik Andreasson, Thomas Jenkins, Peter Brodersen, Stephan Thorgrimsen, Nikolaj H T Petersen, Shijiang Zhu, Jin-Long Qiu, Pernille Micheelsen, Anne Rocher, Morten Petersen, Mari-Anne Newman, Henrik Bjørn Nielsen, Heribert Hirt, Imre Somssich, Ole Mattsson, John Mundy

342 Citations (Scopus)

Abstract

Arabidopsis MAP kinase 4 (MPK4) functions as a regulator of pathogen defense responses, because it is required for both repression of salicylic acid (SA)-dependent resistance and for activation of jasmonate (JA)-dependent defense gene expression. To understand MPK4 signaling mechanisms, we used yeast two-hybrid screening to identify the MPK4 substrate MKS1. Analyses of transgenic plants and genome-wide transcript profiling indicated that MKS1 is required for full SA-dependent resistance in mpk4 mutants, and that overexpression of MKS1 in wild-type plants is sufficient to activate SA-dependent resistance, but does not interfere with induction of a defense gene by JA. Further yeast two-hybrid screening revealed that MKS1 interacts with the WRKY transcription factors WRKY25 and WRKY33. WRKY25 and WRKY33 were shown to be in vitro substrates of MPK4, and a wrky33 knockout mutant was found to exhibit increased expression of the SA-related defense gene PR1. MKS1 may therefore contribute to MPK4-regulated defense activation by coupling the kinase to specific WRKY transcription factors.

Original language	English
Journal	EMBO Journal
Volume	24
Issue number	14
Pages (from-to)	2579-89
Number of pages	10
ISSN	0261-4189
DOIs	https://doi.org/10.1038/sj.emboj.7600737
Publication status	Published - 2005

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Andreasson, E., Jenkins, T., Brodersen, P., Thorgrimsen, S., Petersen, N. H. T., Zhu, S., Qiu, J.-L., Micheelsen, P., Rocher, A., Petersen, M., Newman, M.-A., Bjørn Nielsen, H., Hirt, H., Somssich, I., Mattsson, O., & Mundy, J. (2005). The MAP kinase substrate MKS1 is a regulator of plant defense responses. EMBO Journal, 24(14), 2579-89. https://doi.org/10.1038/sj.emboj.7600737

Andreasson, E, Jenkins, T, Brodersen, P, Thorgrimsen, S, Petersen, NHT, Zhu, S, Qiu, J-L, Micheelsen, P, Rocher, A, Petersen, M , Newman, M-A, Bjørn Nielsen, H, Hirt, H, Somssich, I, Mattsson, O & Mundy, J 2005, 'The MAP kinase substrate MKS1 is a regulator of plant defense responses.', EMBO Journal, vol. 24, no. 14, pp. 2579-89. https://doi.org/10.1038/sj.emboj.7600737

@article{706adcc0954011dd86a6000ea68e967b,

title = "The MAP kinase substrate MKS1 is a regulator of plant defense responses.",

abstract = "Arabidopsis MAP kinase 4 (MPK4) functions as a regulator of pathogen defense responses, because it is required for both repression of salicylic acid (SA)-dependent resistance and for activation of jasmonate (JA)-dependent defense gene expression. To understand MPK4 signaling mechanisms, we used yeast two-hybrid screening to identify the MPK4 substrate MKS1. Analyses of transgenic plants and genome-wide transcript profiling indicated that MKS1 is required for full SA-dependent resistance in mpk4 mutants, and that overexpression of MKS1 in wild-type plants is sufficient to activate SA-dependent resistance, but does not interfere with induction of a defense gene by JA. Further yeast two-hybrid screening revealed that MKS1 interacts with the WRKY transcription factors WRKY25 and WRKY33. WRKY25 and WRKY33 were shown to be in vitro substrates of MPK4, and a wrky33 knockout mutant was found to exhibit increased expression of the SA-related defense gene PR1. MKS1 may therefore contribute to MPK4-regulated defense activation by coupling the kinase to specific WRKY transcription factors.",

author = "Erik Andreasson and Thomas Jenkins and Peter Brodersen and Stephan Thorgrimsen and Petersen, {Nikolaj H T} and Shijiang Zhu and Jin-Long Qiu and Pernille Micheelsen and Anne Rocher and Morten Petersen and Mari-Anne Newman and {Bj{\o}rn Nielsen}, Henrik and Heribert Hirt and Imre Somssich and Ole Mattsson and John Mundy",

note = "Keywords: Amino Acid Motifs; Amino Acid Sequence; Arabidopsis Proteins; Gene Expression Profiling; Immunity, Natural; Immunohistochemistry; Mitogen-Activated Protein Kinases; Molecular Sequence Data; Phosphoproteins; Phosphorylation; Plants; Saccharomyces cerevisiae; Substrate Specificity; Two-Hybrid System Techniques",

year = "2005",

doi = "10.1038/sj.emboj.7600737",

language = "English",

volume = "24",

pages = "2579--89",

journal = "EMBO Journal",

issn = "0261-4189",

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T1 - The MAP kinase substrate MKS1 is a regulator of plant defense responses.

AU - Andreasson, Erik

AU - Jenkins, Thomas

AU - Brodersen, Peter

AU - Thorgrimsen, Stephan

AU - Petersen, Nikolaj H T

AU - Zhu, Shijiang

AU - Qiu, Jin-Long

AU - Micheelsen, Pernille

AU - Rocher, Anne

AU - Petersen, Morten

AU - Newman, Mari-Anne

AU - Bjørn Nielsen, Henrik

AU - Hirt, Heribert

AU - Somssich, Imre

AU - Mattsson, Ole

AU - Mundy, John

N1 - Keywords: Amino Acid Motifs; Amino Acid Sequence; Arabidopsis Proteins; Gene Expression Profiling; Immunity, Natural; Immunohistochemistry; Mitogen-Activated Protein Kinases; Molecular Sequence Data; Phosphoproteins; Phosphorylation; Plants; Saccharomyces cerevisiae; Substrate Specificity; Two-Hybrid System Techniques

PY - 2005

Y1 - 2005

N2 - Arabidopsis MAP kinase 4 (MPK4) functions as a regulator of pathogen defense responses, because it is required for both repression of salicylic acid (SA)-dependent resistance and for activation of jasmonate (JA)-dependent defense gene expression. To understand MPK4 signaling mechanisms, we used yeast two-hybrid screening to identify the MPK4 substrate MKS1. Analyses of transgenic plants and genome-wide transcript profiling indicated that MKS1 is required for full SA-dependent resistance in mpk4 mutants, and that overexpression of MKS1 in wild-type plants is sufficient to activate SA-dependent resistance, but does not interfere with induction of a defense gene by JA. Further yeast two-hybrid screening revealed that MKS1 interacts with the WRKY transcription factors WRKY25 and WRKY33. WRKY25 and WRKY33 were shown to be in vitro substrates of MPK4, and a wrky33 knockout mutant was found to exhibit increased expression of the SA-related defense gene PR1. MKS1 may therefore contribute to MPK4-regulated defense activation by coupling the kinase to specific WRKY transcription factors.

AB - Arabidopsis MAP kinase 4 (MPK4) functions as a regulator of pathogen defense responses, because it is required for both repression of salicylic acid (SA)-dependent resistance and for activation of jasmonate (JA)-dependent defense gene expression. To understand MPK4 signaling mechanisms, we used yeast two-hybrid screening to identify the MPK4 substrate MKS1. Analyses of transgenic plants and genome-wide transcript profiling indicated that MKS1 is required for full SA-dependent resistance in mpk4 mutants, and that overexpression of MKS1 in wild-type plants is sufficient to activate SA-dependent resistance, but does not interfere with induction of a defense gene by JA. Further yeast two-hybrid screening revealed that MKS1 interacts with the WRKY transcription factors WRKY25 and WRKY33. WRKY25 and WRKY33 were shown to be in vitro substrates of MPK4, and a wrky33 knockout mutant was found to exhibit increased expression of the SA-related defense gene PR1. MKS1 may therefore contribute to MPK4-regulated defense activation by coupling the kinase to specific WRKY transcription factors.

U2 - 10.1038/sj.emboj.7600737

DO - 10.1038/sj.emboj.7600737

M3 - Journal article

C2 - 15990873

SN - 0261-4189

VL - 24

SP - 2579

EP - 2589

JO - EMBO Journal

JF - EMBO Journal

IS - 14

ER -

The MAP kinase substrate MKS1 is a regulator of plant defense responses.

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