The macrophage system in the intestinal muscularis externa during inflammation: an immunohistochemical and quantitative study of osteopetrotic mice.

TY - JOUR

T1 - The macrophage system in the intestinal muscularis externa during inflammation: an immunohistochemical and quantitative study of osteopetrotic mice.

AU - Mikkelsen, Hanne Birte

AU - Larsen, Jytte Overgaard

AU - Hadberg, Hanne

PY - 2008

Y1 - 2008

N2 - Intestinal inflammation results in disturbed intestinal motility in humans as well as in animal models. This altered function of smooth muscle cells and/or the enteric nervous system may be caused by activation of macrophages in muscularis externa and a thereby following release of cytokines and chemokines that causes influx of mononuclear cells and neutrophilic granulocytes. We subjected osteopetrotic (op/op) mice that lack certain macrophage subtypes, e.g. macrophages in the muscularis externa and +/+ mice to LPS to induce inflammatory cell influx. The densities of F4/80(+), MHCII(+), and myeloperoxidase(+) cells were quantified using stereological sampling. In +/+ mice we found that MHCII(+) cells outnumber F4/80(+) cells and that LPS injection increased the density of MHCII(+) cells temporarily but not that of F4/80(+) cells. This indicates that an upregulation of MHCII antigen takes place and that two or more macrophage subtypes with comparable morphologies exist. Osteopetrotic mice lacked MHCII(+), CD169(+), and F4/80(+) cells after either treatment, which indicate that these cells are CSF-1-dependent. LPS induced VCAM-1 activation of the vessels, modest influx of granulocytes, as well as an iNOS-activation in a cell type different from macrophages in both +/+ and op/op mice.

AB - Intestinal inflammation results in disturbed intestinal motility in humans as well as in animal models. This altered function of smooth muscle cells and/or the enteric nervous system may be caused by activation of macrophages in muscularis externa and a thereby following release of cytokines and chemokines that causes influx of mononuclear cells and neutrophilic granulocytes. We subjected osteopetrotic (op/op) mice that lack certain macrophage subtypes, e.g. macrophages in the muscularis externa and +/+ mice to LPS to induce inflammatory cell influx. The densities of F4/80(+), MHCII(+), and myeloperoxidase(+) cells were quantified using stereological sampling. In +/+ mice we found that MHCII(+) cells outnumber F4/80(+) cells and that LPS injection increased the density of MHCII(+) cells temporarily but not that of F4/80(+) cells. This indicates that an upregulation of MHCII antigen takes place and that two or more macrophage subtypes with comparable morphologies exist. Osteopetrotic mice lacked MHCII(+), CD169(+), and F4/80(+) cells after either treatment, which indicate that these cells are CSF-1-dependent. LPS induced VCAM-1 activation of the vessels, modest influx of granulocytes, as well as an iNOS-activation in a cell type different from macrophages in both +/+ and op/op mice.

U2 - 10.1007/s00418-008-0423-x

DO - 10.1007/s00418-008-0423-x

M3 - Journal article

C2 - 18392842

SN - 0948-6143

VL - 130

SP - 363

EP - 373

JO - Histochemistry and Cell Biology

JF - Histochemistry and Cell Biology

IS - 2

ER -