The isoelectric point of proteins influences their translocation to the extrahaustorial matrix of the barley powdery mildew fungus

Lara Smigielski; Geziel B. Aguilar; Mark Kwaaitaal; Wen‐jing Zhang; Hans Thordal‐Christensen

doi:10.1111/cmi.13091

The isoelectric point of proteins influences their translocation to the extrahaustorial matrix of the barley powdery mildew fungus

Lara Smigielski, Geziel B. Aguilar, Mark Kwaaitaal, Wen‐jing Zhang, Hans Thordal‐Christensen^*

^*Corresponding author for this work

Section for Plant and Soil Sciences

2 Citations (Scopus)

32 Downloads (Pure)

Abstract

Many biotrophic fungal plant pathogens develop feeding structures, haustoria, inside living plant cells, which are essential for their success. Extrahaustorial membranes (EHMs) surround haustoria and delimit the extrahaustorial matrices (EHMxs). Little is known about transport mechanisms across EHMs and what properties proteins and nutrients need in order to cross these membranes. To investigate this further, we expressed fluorescent proteins in the cytosol of infected barley leaf epidermal cells after particle bombardment and investigated properties that influenced their localisation in the powdery mildew EHMx. We showed that this translocation is favoured by a neutral isoelectric point (pI) between 6.0 and 8.4. However, for proteins larger than 50 kDa, pI alone does not explain their localisation, hinting towards a more complex interplay between pI, size, and sequence properties. We discuss the possibility that an EHM translocon is involved in protein uptake into the EHMx.

Original language	English
Journal	Cellular Microbiology
Number of pages	31
ISSN	1462-5814
DOIs	https://doi.org/10.1111/cmi.13091
Publication status	Published - 1 Dec 2019

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title = "The isoelectric point of proteins influences their translocation to the extrahaustorial matrix of the barley powdery mildew fungus",

abstract = "Many biotrophic fungal plant pathogens develop feeding structures, haustoria, inside living plant cells, which are essential for their success. Extrahaustorial membranes (EHMs) surround haustoria and delimit the extrahaustorial matrices (EHMxs). Little is known about transport mechanisms across EHMs and what properties proteins and nutrients need in order to cross these membranes. To investigate this further, we expressed fluorescent proteins in the cytosol of infected barley leaf epidermal cells after particle bombardment and investigated properties that influenced their localisation in the powdery mildew EHMx. We showed that this translocation is favoured by a neutral isoelectric point (pI) between 6.0 and 8.4. However, for proteins larger than 50 kDa, pI alone does not explain their localisation, hinting towards a more complex interplay between pI, size, and sequence properties. We discuss the possibility that an EHM translocon is involved in protein uptake into the EHMx.",

author = "Lara Smigielski and Aguilar, {Geziel B.} and Mark Kwaaitaal and Wen‐jing Zhang and Hans Thordal‐Christensen",

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T1 - The isoelectric point of proteins influences their translocation to the extrahaustorial matrix of the barley powdery mildew fungus

AU - Smigielski, Lara

AU - Aguilar, Geziel B.

AU - Kwaaitaal, Mark

AU - Zhang, Wen‐jing

AU - Thordal‐Christensen, Hans

PY - 2019/12/1

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N2 - Many biotrophic fungal plant pathogens develop feeding structures, haustoria, inside living plant cells, which are essential for their success. Extrahaustorial membranes (EHMs) surround haustoria and delimit the extrahaustorial matrices (EHMxs). Little is known about transport mechanisms across EHMs and what properties proteins and nutrients need in order to cross these membranes. To investigate this further, we expressed fluorescent proteins in the cytosol of infected barley leaf epidermal cells after particle bombardment and investigated properties that influenced their localisation in the powdery mildew EHMx. We showed that this translocation is favoured by a neutral isoelectric point (pI) between 6.0 and 8.4. However, for proteins larger than 50 kDa, pI alone does not explain their localisation, hinting towards a more complex interplay between pI, size, and sequence properties. We discuss the possibility that an EHM translocon is involved in protein uptake into the EHMx.

AB - Many biotrophic fungal plant pathogens develop feeding structures, haustoria, inside living plant cells, which are essential for their success. Extrahaustorial membranes (EHMs) surround haustoria and delimit the extrahaustorial matrices (EHMxs). Little is known about transport mechanisms across EHMs and what properties proteins and nutrients need in order to cross these membranes. To investigate this further, we expressed fluorescent proteins in the cytosol of infected barley leaf epidermal cells after particle bombardment and investigated properties that influenced their localisation in the powdery mildew EHMx. We showed that this translocation is favoured by a neutral isoelectric point (pI) between 6.0 and 8.4. However, for proteins larger than 50 kDa, pI alone does not explain their localisation, hinting towards a more complex interplay between pI, size, and sequence properties. We discuss the possibility that an EHM translocon is involved in protein uptake into the EHMx.

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DO - 10.1111/cmi.13091

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The isoelectric point of proteins influences their translocation to the extrahaustorial matrix of the barley powdery mildew fungus

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