The Arabidopsis NPF3 protein is a GA transporter

Iris Tal; Yi Zhang; Morten Egevang Jørgensen; Odelia Pisanty; Inês C R Barbosa; Melina Zourelidou; Thomas Regnault; Christoph Crocoll; Carl Erik Olsen; Roy Weinstain; Claus Schwechheimer; Barbara Ann Halkier; Hussam Hassan Nour-Eldin; Mark Estelle; Eilon Shani

doi:10.1038/ncomms11486

The Arabidopsis NPF3 protein is a GA transporter

Iris Tal, Yi Zhang, Morten Egevang Jørgensen, Odelia Pisanty, Inês C R Barbosa, Melina Zourelidou, Thomas Regnault, Christoph Crocoll, Carl Erik Olsen, Roy Weinstain, Claus Schwechheimer, Barbara Ann Halkier, Hussam Hassan Nour-Eldin, Mark Estelle, Eilon Shani

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Abstract

Gibberellins (GAs) are plant hormones that promote a wide range of developmental processes. While GA signalling is well understood, little is known about how GA is transported or how GA distribution is regulated. Here we utilize fluorescently labelled GAs (GA-Fl) to screen for Arabidopsis mutants deficient in GA transport. We show that the NPF3 transporter efficiently transports GA across cell membranes in vitro and GA-Fl in vivo. NPF3 is expressed in root endodermis and repressed by GA. NPF3 is targeted to the plasma membrane and subject to rapid BFA-dependent recycling. We show that abscisic acid (ABA), an antagonist of GA, is also transported by NPF3 in vitro. ABA promotes NPF3 expression and GA-Fl uptake in plants. On the basis of these results, we propose that GA distribution and activity in Arabidopsis is partly regulated by NPF3 acting as an influx carrier and that GA-ABA interaction may occur at the level of transport.

Original language	English
Article number	11486
Journal	Nature Communications
Volume	7
Number of pages	20
ISSN	2041-1723
DOIs	https://doi.org/10.1038/ncomms11486
Publication status	Published - 3 May 2016

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title = "The Arabidopsis NPF3 protein is a GA transporter",

abstract = "Gibberellins (GAs) are plant hormones that promote a wide range of developmental processes. While GA signalling is well understood, little is known about how GA is transported or how GA distribution is regulated. Here we utilize fluorescently labelled GAs (GA-Fl) to screen for Arabidopsis mutants deficient in GA transport. We show that the NPF3 transporter efficiently transports GA across cell membranes in vitro and GA-Fl in vivo. NPF3 is expressed in root endodermis and repressed by GA. NPF3 is targeted to the plasma membrane and subject to rapid BFA-dependent recycling. We show that abscisic acid (ABA), an antagonist of GA, is also transported by NPF3 in vitro. ABA promotes NPF3 expression and GA-Fl uptake in plants. On the basis of these results, we propose that GA distribution and activity in Arabidopsis is partly regulated by NPF3 acting as an influx carrier and that GA-ABA interaction may occur at the level of transport.",

author = "Iris Tal and Yi Zhang and J{\o}rgensen, {Morten Egevang} and Odelia Pisanty and Barbosa, {In{\^e}s C R} and Melina Zourelidou and Thomas Regnault and Christoph Crocoll and Olsen, {Carl Erik} and Roy Weinstain and Claus Schwechheimer and Halkier, {Barbara Ann} and Nour-Eldin, {Hussam Hassan} and Mark Estelle and Eilon Shani",

year = "2016",

month = may,

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doi = "10.1038/ncomms11486",

language = "English",

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T1 - The Arabidopsis NPF3 protein is a GA transporter

AU - Tal, Iris

AU - Zhang, Yi

AU - Jørgensen, Morten Egevang

AU - Pisanty, Odelia

AU - Barbosa, Inês C R

AU - Zourelidou, Melina

AU - Regnault, Thomas

AU - Crocoll, Christoph

AU - Olsen, Carl Erik

AU - Weinstain, Roy

AU - Schwechheimer, Claus

AU - Halkier, Barbara Ann

AU - Nour-Eldin, Hussam Hassan

AU - Estelle, Mark

AU - Shani, Eilon

PY - 2016/5/3

Y1 - 2016/5/3

N2 - Gibberellins (GAs) are plant hormones that promote a wide range of developmental processes. While GA signalling is well understood, little is known about how GA is transported or how GA distribution is regulated. Here we utilize fluorescently labelled GAs (GA-Fl) to screen for Arabidopsis mutants deficient in GA transport. We show that the NPF3 transporter efficiently transports GA across cell membranes in vitro and GA-Fl in vivo. NPF3 is expressed in root endodermis and repressed by GA. NPF3 is targeted to the plasma membrane and subject to rapid BFA-dependent recycling. We show that abscisic acid (ABA), an antagonist of GA, is also transported by NPF3 in vitro. ABA promotes NPF3 expression and GA-Fl uptake in plants. On the basis of these results, we propose that GA distribution and activity in Arabidopsis is partly regulated by NPF3 acting as an influx carrier and that GA-ABA interaction may occur at the level of transport.

AB - Gibberellins (GAs) are plant hormones that promote a wide range of developmental processes. While GA signalling is well understood, little is known about how GA is transported or how GA distribution is regulated. Here we utilize fluorescently labelled GAs (GA-Fl) to screen for Arabidopsis mutants deficient in GA transport. We show that the NPF3 transporter efficiently transports GA across cell membranes in vitro and GA-Fl in vivo. NPF3 is expressed in root endodermis and repressed by GA. NPF3 is targeted to the plasma membrane and subject to rapid BFA-dependent recycling. We show that abscisic acid (ABA), an antagonist of GA, is also transported by NPF3 in vitro. ABA promotes NPF3 expression and GA-Fl uptake in plants. On the basis of these results, we propose that GA distribution and activity in Arabidopsis is partly regulated by NPF3 acting as an influx carrier and that GA-ABA interaction may occur at the level of transport.

U2 - 10.1038/ncomms11486

DO - 10.1038/ncomms11486

M3 - Journal article

C2 - 27139299

SN - 2041-1723

VL - 7

JO - Nature Communications

JF - Nature Communications

M1 - 11486

ER -

The Arabidopsis NPF3 protein is a GA transporter

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