The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development

Carsten Rautengarten; Berit Ebert; Lifeng Liu; Solomon Stonebloom; Andreia M Smith-Moritz; Markus Pauly; Ariel Orellana; Henrik Vibe Scheller; Joshua L Heazlewood

doi:10.1038/ncomms12119

The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development

Carsten Rautengarten, Berit Ebert, Lifeng Liu, Solomon Stonebloom, Andreia M Smith-Moritz, Markus Pauly, Ariel Orellana, Henrik Vibe Scheller, Joshua L Heazlewood

Section for Plant Glycobiology

32 Citations (Scopus)

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Abstract

Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.

Original language	English
Article number	12119
Journal	Nature Communications
Volume	7
Number of pages	10
ISSN	2041-1723
DOIs	https://doi.org/10.1038/ncomms12119
Publication status	Published - 6 Jul 2016

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title = "The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development",

abstract = "Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.",

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AU - Rautengarten, Carsten

AU - Ebert, Berit

AU - Liu, Lifeng

AU - Stonebloom, Solomon

AU - Smith-Moritz, Andreia M

AU - Pauly, Markus

AU - Orellana, Ariel

AU - Scheller, Henrik Vibe

AU - Heazlewood, Joshua L

PY - 2016/7/6

Y1 - 2016/7/6

N2 - Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.

AB - Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.

KW - Journal Article

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DO - 10.1038/ncomms12119

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The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development

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