Abstract
In sporadic ovarian cancer, we have previously reported allele loss at D6S193 (62%) on chromosome 6q27, which suggested the presence of a putative tumour suppressor gene. Based on our data and that from another group, the minimal region of allele loss was between D6S264 and D6S149 (7.4 cM). To identify the putative tumour suppressor gene, we established a physical map initially with YACs and subsequently with PACs/BACs from D6S264 to D6S149. To accelerate the identification of genes, we sequenced the entire contig of approximately 1.1 Mb. Seven genes were identified within the region of allele loss between D6S264 and D6S149.
| Original language | English |
|---|---|
| Journal | B M C Genetics |
| Volume | 3 |
| Pages (from-to) | 20 |
| ISSN | 1471-2156 |
| Publication status | Published - 15 Oct 2002 |
Keywords
- Amino Acid Sequence
- Base Sequence
- Cell Line
- Cell Membrane
- Chromatography, High Pressure Liquid
- Chromosome Mapping
- Chromosomes, Human, Pair 6
- DNA Mutational Analysis
- DNA, Complementary
- DNA, Neoplasm
- Female
- Gene Expression Regulation, Neoplastic
- Genes, Tumor Suppressor
- Green Fluorescent Proteins
- Humans
- Luminescent Proteins
- Membrane Proteins
- Molecular Sequence Data
- Muscle Proteins
- Mutation
- Ovarian Neoplasms
- Polymorphism, Single-Stranded Conformational
- Recombinant Fusion Proteins
- Sequence Homology, Amino Acid
- Tumor Cells, Cultured
