The fibronectin-binding integrins alpha5beta1 and alphavbeta3 differentially modulate RhoA-GTP loading, organization of cell matrix adhesions, and fibronectin fibrillogenesis.

Erik H J Danen; Petra Sonneveld; Cord Brakebusch; Reinhard Fassler; Arnoud Sonnenberg

doi:10.1083/jcb.200205014

The fibronectin-binding integrins alpha5beta1 and alphavbeta3 differentially modulate RhoA-GTP loading, organization of cell matrix adhesions, and fibronectin fibrillogenesis.

Erik H J Danen, Petra Sonneveld, Cord Brakebusch, Reinhard Fassler, Arnoud Sonnenberg

Department of Biomedical Sciences

236 Citations (Scopus)

Abstract

We have studied the formation of different types of cell matrix adhesions in cells that bind to fibronectin via either alpha5beta1 or alphavbeta3. In both cases, cell adhesion to fibronectin leads to a rapid decrease in RhoA activity. However, alpha5beta1 but not alphavbeta3 supports high levels of RhoA activity at later stages of cell spreading, which are associated with a translocation of focal contacts to peripheral cell protrusions, recruitment of tensin into fibrillar adhesions, and fibronectin fibrillogenesis. Expression of an activated mutant of RhoA stimulates alphavbeta3-mediated fibrillogenesis. Despite the fact that alpha5beta1-mediated adhesion to the central cell-binding domain of fibronectin supports activation of RhoA, other regions of fibronectin are required for the development of alpha5beta1-mediated but not alphavbeta3-mediated focal contacts. Using chimeras of beta1 and beta3 subunits, we find that the extracellular domain of beta1 controls RhoA activity. By expressing both beta1 and beta3 at high levels, we show that beta1-mediated control of the levels of beta3 is important for the distribution of focal contacts. Our findings demonstrate that the pattern of fibronectin receptors expressed on a cell dictates the ability of fibronectin to stimulate RhoA-mediated organization of cell matrix adhesions.

Original language	English
Journal	Journal of Cell Biology
Volume	159
Issue number	6
Pages (from-to)	1071-86
Number of pages	15
ISSN	0021-9525
DOIs	https://doi.org/10.1083/jcb.200205014
Publication status	Published - 2002

Access to Document

10.1083/jcb.200205014

Cite this

The fibronectin-binding integrins alpha5beta1 and alphavbeta3 differentially modulate RhoA-GTP loading, organization of cell matrix adhesions, and fibronectin fibrillogenesis. / Danen, Erik H J; Sonneveld, Petra; Brakebusch, Cord et al.

In: Journal of Cell Biology, Vol. 159, No. 6, 2002, p. 1071-86.

Research output: Contribution to journal › Journal article › Research › peer-review

@article{2497f4e0589611dd8d9f000ea68e967b,

title = "The fibronectin-binding integrins alpha5beta1 and alphavbeta3 differentially modulate RhoA-GTP loading, organization of cell matrix adhesions, and fibronectin fibrillogenesis.",

abstract = "We have studied the formation of different types of cell matrix adhesions in cells that bind to fibronectin via either alpha5beta1 or alphavbeta3. In both cases, cell adhesion to fibronectin leads to a rapid decrease in RhoA activity. However, alpha5beta1 but not alphavbeta3 supports high levels of RhoA activity at later stages of cell spreading, which are associated with a translocation of focal contacts to peripheral cell protrusions, recruitment of tensin into fibrillar adhesions, and fibronectin fibrillogenesis. Expression of an activated mutant of RhoA stimulates alphavbeta3-mediated fibrillogenesis. Despite the fact that alpha5beta1-mediated adhesion to the central cell-binding domain of fibronectin supports activation of RhoA, other regions of fibronectin are required for the development of alpha5beta1-mediated but not alphavbeta3-mediated focal contacts. Using chimeras of beta1 and beta3 subunits, we find that the extracellular domain of beta1 controls RhoA activity. By expressing both beta1 and beta3 at high levels, we show that beta1-mediated control of the levels of beta3 is important for the distribution of focal contacts. Our findings demonstrate that the pattern of fibronectin receptors expressed on a cell dictates the ability of fibronectin to stimulate RhoA-mediated organization of cell matrix adhesions.",

author = "Danen, {Erik H J} and Petra Sonneveld and Cord Brakebusch and Reinhard Fassler and Arnoud Sonnenberg",

note = "Keywords: Actins; Animals; Cell Adhesion; Cell Separation; DNA, Complementary; DNA-Binding Proteins; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Fibrin; Fibronectins; Flow Cytometry; Focal Adhesion Kinase 1; Focal Adhesion Protein-Tyrosine Kinases; GTPase-Activating Proteins; Gene Expression Regulation; Guanine Nucleotide Exchange Factors; Guanosine Triphosphate; Humans; Integrin alpha5beta1; Integrin alphaVbeta3; Integrins; Mice; Microfilament Proteins; Microscopy, Fluorescence; Nuclear Proteins; Phenotype; Phosphoproteins; Phosphorylation; Protein Binding; Protein Structure, Tertiary; Protein-Tyrosine Kinases; Proteins; Repressor Proteins; Retinoblastoma-Like Protein p130; Time Factors; Transfection; Vinculin; rhoA GTP-Binding Protein",

year = "2002",

doi = "10.1083/jcb.200205014",

language = "English",

volume = "159",

pages = "1071--86",

journal = "Journal of Cell Biology",

issn = "0021-9525",

publisher = "Rockefeller University Press",

number = "6",

}

TY - JOUR

T1 - The fibronectin-binding integrins alpha5beta1 and alphavbeta3 differentially modulate RhoA-GTP loading, organization of cell matrix adhesions, and fibronectin fibrillogenesis.

AU - Danen, Erik H J

AU - Sonneveld, Petra

AU - Brakebusch, Cord

AU - Fassler, Reinhard

AU - Sonnenberg, Arnoud

N1 - Keywords: Actins; Animals; Cell Adhesion; Cell Separation; DNA, Complementary; DNA-Binding Proteins; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Fibrin; Fibronectins; Flow Cytometry; Focal Adhesion Kinase 1; Focal Adhesion Protein-Tyrosine Kinases; GTPase-Activating Proteins; Gene Expression Regulation; Guanine Nucleotide Exchange Factors; Guanosine Triphosphate; Humans; Integrin alpha5beta1; Integrin alphaVbeta3; Integrins; Mice; Microfilament Proteins; Microscopy, Fluorescence; Nuclear Proteins; Phenotype; Phosphoproteins; Phosphorylation; Protein Binding; Protein Structure, Tertiary; Protein-Tyrosine Kinases; Proteins; Repressor Proteins; Retinoblastoma-Like Protein p130; Time Factors; Transfection; Vinculin; rhoA GTP-Binding Protein

PY - 2002

Y1 - 2002

N2 - We have studied the formation of different types of cell matrix adhesions in cells that bind to fibronectin via either alpha5beta1 or alphavbeta3. In both cases, cell adhesion to fibronectin leads to a rapid decrease in RhoA activity. However, alpha5beta1 but not alphavbeta3 supports high levels of RhoA activity at later stages of cell spreading, which are associated with a translocation of focal contacts to peripheral cell protrusions, recruitment of tensin into fibrillar adhesions, and fibronectin fibrillogenesis. Expression of an activated mutant of RhoA stimulates alphavbeta3-mediated fibrillogenesis. Despite the fact that alpha5beta1-mediated adhesion to the central cell-binding domain of fibronectin supports activation of RhoA, other regions of fibronectin are required for the development of alpha5beta1-mediated but not alphavbeta3-mediated focal contacts. Using chimeras of beta1 and beta3 subunits, we find that the extracellular domain of beta1 controls RhoA activity. By expressing both beta1 and beta3 at high levels, we show that beta1-mediated control of the levels of beta3 is important for the distribution of focal contacts. Our findings demonstrate that the pattern of fibronectin receptors expressed on a cell dictates the ability of fibronectin to stimulate RhoA-mediated organization of cell matrix adhesions.

AB - We have studied the formation of different types of cell matrix adhesions in cells that bind to fibronectin via either alpha5beta1 or alphavbeta3. In both cases, cell adhesion to fibronectin leads to a rapid decrease in RhoA activity. However, alpha5beta1 but not alphavbeta3 supports high levels of RhoA activity at later stages of cell spreading, which are associated with a translocation of focal contacts to peripheral cell protrusions, recruitment of tensin into fibrillar adhesions, and fibronectin fibrillogenesis. Expression of an activated mutant of RhoA stimulates alphavbeta3-mediated fibrillogenesis. Despite the fact that alpha5beta1-mediated adhesion to the central cell-binding domain of fibronectin supports activation of RhoA, other regions of fibronectin are required for the development of alpha5beta1-mediated but not alphavbeta3-mediated focal contacts. Using chimeras of beta1 and beta3 subunits, we find that the extracellular domain of beta1 controls RhoA activity. By expressing both beta1 and beta3 at high levels, we show that beta1-mediated control of the levels of beta3 is important for the distribution of focal contacts. Our findings demonstrate that the pattern of fibronectin receptors expressed on a cell dictates the ability of fibronectin to stimulate RhoA-mediated organization of cell matrix adhesions.

U2 - 10.1083/jcb.200205014

DO - 10.1083/jcb.200205014

M3 - Journal article

C2 - 12486108

SN - 0021-9525

VL - 159

SP - 1071

EP - 1086

JO - Journal of Cell Biology

JF - Journal of Cell Biology

IS - 6

ER -

The fibronectin-binding integrins alpha5beta1 and alphavbeta3 differentially modulate RhoA-GTP loading, organization of cell matrix adhesions, and fibronectin fibrillogenesis.

Abstract

Access to Document

Fingerprint

Cite this