The eps15 homology (EH) domain-based interaction between eps15 and hrb connects the molecular machinery of endocytosis to that of nucleocytosolic transport.

TY - JOUR

T1 - The eps15 homology (EH) domain-based interaction between eps15 and hrb connects the molecular machinery of endocytosis to that of nucleocytosolic transport.

AU - Doria, M

AU - Salcini, A E

AU - Colombo, E

AU - Parslow, T G

AU - Pelicci, P G

AU - Di Fiore, P P

N1 - Keywords: Animals; Biological Transport; Calcium-Binding Proteins; Cell Compartmentation; Cell Line; Cell Nucleus; Cytosol; Drug Synergism; Endocytosis; Gene Products, rev; Nuclear Pore Complex Proteins; Nuclear Proteins; Phosphoproteins; RNA-Binding Proteins; Sequence Homology, Amino Acid; Signal Transduction

PY - 1999

Y1 - 1999

N2 - The Eps15 homology (EH) module is a protein-protein interaction domain that establishes a network of connections involved in various aspects of endocytosis and sorting. The finding that EH-containing proteins bind to Hrb (a cellular cofactor of the Rev protein) and to the related protein Hrbl raised the possibility that the EH network might also influence the so-called Rev export pathway, which mediates nucleocytoplasmic transfer of proteins and RNAs. In this study, we demonstrate that Eps15 and Eps15R, two EH-containing proteins, synergize with Hrb and Hrbl to enhance the function of Rev in the export pathway. In addition, the EH-mediated association between Eps15 and Hrb is required for the synergistic effect. The interaction between Eps15 and Hrb occurs in the cytoplasm, thus pointing to an unexpected site of action of Hrb, and to a possible role of the Eps15-Hrb complex in regulating the stability of Rev.

AB - The Eps15 homology (EH) module is a protein-protein interaction domain that establishes a network of connections involved in various aspects of endocytosis and sorting. The finding that EH-containing proteins bind to Hrb (a cellular cofactor of the Rev protein) and to the related protein Hrbl raised the possibility that the EH network might also influence the so-called Rev export pathway, which mediates nucleocytoplasmic transfer of proteins and RNAs. In this study, we demonstrate that Eps15 and Eps15R, two EH-containing proteins, synergize with Hrb and Hrbl to enhance the function of Rev in the export pathway. In addition, the EH-mediated association between Eps15 and Hrb is required for the synergistic effect. The interaction between Eps15 and Hrb occurs in the cytoplasm, thus pointing to an unexpected site of action of Hrb, and to a possible role of the Eps15-Hrb complex in regulating the stability of Rev.

M3 - Journal article

C2 - 10613896

SN - 0021-9525

VL - 147

SP - 1379

EP - 1384

JO - Journal of Cell Biology

JF - Journal of Cell Biology

IS - 7

ER -