The Effect of Lean-Seafood and Non-Seafood Diets on Fecal Metabolites and Gut Microbiome: Results from a Randomized Crossover Intervention Study

Mette Schmedes; Asker Daniel Brejnrod; Eli Kristin Aadland; Pia Kiilerich; Karsten Kristiansen; Hélène Jacques; Charles Lavigne; Ingvild Eide Graff; Øyvin Eng; Asle Holthe; Gunnar Mellgren; Jette Feveile Young; Ulrik Kræmer Sundekilde; Bjørn Liaset; Hanne Christine Bertram

doi:10.1002/mnfr.201700976

The Effect of Lean-Seafood and Non-Seafood Diets on Fecal Metabolites and Gut Microbiome: Results from a Randomized Crossover Intervention Study

Mette Schmedes, Asker Daniel Brejnrod, Eli Kristin Aadland, Pia Kiilerich, Karsten Kristiansen, Hélène Jacques, Charles Lavigne, Ingvild Eide Graff, Øyvin Eng, Asle Holthe, Gunnar Mellgren, Jette Feveile Young, Ulrik Kræmer Sundekilde, Bjørn Liaset, Hanne Christine Bertram^*

^*Corresponding author for this work

17 Citations (Scopus)

Abstract

Scope: The impact of dietary protein types on the gut microbiome is scarcely studied. The aim of the present study is therefore to examine the effects of lean-seafood and non-seafood proteins on the gut microbiome composition and activity and elucidate potential associations to cardiovascular disease (CVD) risk factors. Methods: A crossover intervention study in which 20 healthy subjects consumed two diets that varied in protein source was conducted. ¹H NMR spectroscopy and 16S rDNA sequencing analyses were applied to characterize fecal metabolites and gut microbiota composition, respectively. Results: A twofold increase in fecal trimethylamine excretion was observed after the lean-seafood diet period. Circulating TAG and the total to high-density lipoprotein (HDL) cholesterol ratio as well as circulating TMAO levels were each associated with specific gut bacteria. Following the non-seafood diet period, a decreased relative abundance of Clostridium cluster IV and a tendency toward an increased Firmicutes/Bacteroidetes ratio were found. Conclusions: Lean-seafood and non-seafood diets differentially modulate the gut microbiome composition and activity. Furthermore, the gut microbiota composition seems to affect circulating TMAO levels and CVD risk factors.

Original language	English
Article number	1700976
Journal	Molecular Nutrition and Food Research
Volume	63
Issue number	1
Pages (from-to)	1-8
ISSN	1613-4125
DOIs	https://doi.org/10.1002/mnfr.201700976
Publication status	Published - 2019

Keywords

16S rDNA
feces
gut microbiota
seafood protein
TMAO

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Schmedes, M., Brejnrod, A. D., Aadland, E. K., Kiilerich, P., Kristiansen, K., Jacques, H., Lavigne, C., Graff, I. E., Eng, Ø., Holthe, A., Mellgren, G., Young, J. F., Sundekilde, U. K., Liaset, B., & Bertram, H. C. (2019). The Effect of Lean-Seafood and Non-Seafood Diets on Fecal Metabolites and Gut Microbiome: Results from a Randomized Crossover Intervention Study. Molecular Nutrition and Food Research, 63(1), 1-8. [1700976]. https://doi.org/10.1002/mnfr.201700976

The Effect of Lean-Seafood and Non-Seafood Diets on Fecal Metabolites and Gut Microbiome : Results from a Randomized Crossover Intervention Study. / Schmedes, Mette; Brejnrod, Asker Daniel; Aadland, Eli Kristin et al.

In: Molecular Nutrition and Food Research, Vol. 63, No. 1, 1700976, 2019, p. 1-8.

Research output: Contribution to journal › Journal article › Research › peer-review

Schmedes, M, Brejnrod, AD, Aadland, EK, Kiilerich, P, Kristiansen, K, Jacques, H, Lavigne, C, Graff, IE, Eng, Ø, Holthe, A, Mellgren, G, Young, JF, Sundekilde, UK, Liaset, B & Bertram, HC 2019, 'The Effect of Lean-Seafood and Non-Seafood Diets on Fecal Metabolites and Gut Microbiome: Results from a Randomized Crossover Intervention Study', Molecular Nutrition and Food Research, vol. 63, no. 1, 1700976, pp. 1-8. https://doi.org/10.1002/mnfr.201700976

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title = "The Effect of Lean-Seafood and Non-Seafood Diets on Fecal Metabolites and Gut Microbiome: Results from a Randomized Crossover Intervention Study",

abstract = "Scope: The impact of dietary protein types on the gut microbiome is scarcely studied. The aim of the present study is therefore to examine the effects of lean-seafood and non-seafood proteins on the gut microbiome composition and activity and elucidate potential associations to cardiovascular disease (CVD) risk factors. Methods: A crossover intervention study in which 20 healthy subjects consumed two diets that varied in protein source was conducted. 1H NMR spectroscopy and 16S rDNA sequencing analyses were applied to characterize fecal metabolites and gut microbiota composition, respectively. Results: A twofold increase in fecal trimethylamine excretion was observed after the lean-seafood diet period. Circulating TAG and the total to high-density lipoprotein (HDL) cholesterol ratio as well as circulating TMAO levels were each associated with specific gut bacteria. Following the non-seafood diet period, a decreased relative abundance of Clostridium cluster IV and a tendency toward an increased Firmicutes/Bacteroidetes ratio were found. Conclusions: Lean-seafood and non-seafood diets differentially modulate the gut microbiome composition and activity. Furthermore, the gut microbiota composition seems to affect circulating TMAO levels and CVD risk factors.",

keywords = "16S rDNA, feces, gut microbiota, seafood protein, TMAO",

author = "Mette Schmedes and Brejnrod, {Asker Daniel} and Aadland, {Eli Kristin} and Pia Kiilerich and Karsten Kristiansen and H{\'e}l{\`e}ne Jacques and Charles Lavigne and Graff, {Ingvild Eide} and {\O}yvin Eng and Asle Holthe and Gunnar Mellgren and Young, {Jette Feveile} and Sundekilde, {Ulrik Kr{\ae}mer} and Bj{\o}rn Liaset and Bertram, {Hanne Christine}",

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T1 - The Effect of Lean-Seafood and Non-Seafood Diets on Fecal Metabolites and Gut Microbiome

T2 - Results from a Randomized Crossover Intervention Study

AU - Schmedes, Mette

AU - Brejnrod, Asker Daniel

AU - Aadland, Eli Kristin

AU - Kiilerich, Pia

AU - Kristiansen, Karsten

AU - Jacques, Hélène

AU - Lavigne, Charles

AU - Graff, Ingvild Eide

AU - Eng, Øyvin

AU - Holthe, Asle

AU - Mellgren, Gunnar

AU - Young, Jette Feveile

AU - Sundekilde, Ulrik Kræmer

AU - Liaset, Bjørn

AU - Bertram, Hanne Christine

PY - 2019

Y1 - 2019

N2 - Scope: The impact of dietary protein types on the gut microbiome is scarcely studied. The aim of the present study is therefore to examine the effects of lean-seafood and non-seafood proteins on the gut microbiome composition and activity and elucidate potential associations to cardiovascular disease (CVD) risk factors. Methods: A crossover intervention study in which 20 healthy subjects consumed two diets that varied in protein source was conducted. 1H NMR spectroscopy and 16S rDNA sequencing analyses were applied to characterize fecal metabolites and gut microbiota composition, respectively. Results: A twofold increase in fecal trimethylamine excretion was observed after the lean-seafood diet period. Circulating TAG and the total to high-density lipoprotein (HDL) cholesterol ratio as well as circulating TMAO levels were each associated with specific gut bacteria. Following the non-seafood diet period, a decreased relative abundance of Clostridium cluster IV and a tendency toward an increased Firmicutes/Bacteroidetes ratio were found. Conclusions: Lean-seafood and non-seafood diets differentially modulate the gut microbiome composition and activity. Furthermore, the gut microbiota composition seems to affect circulating TMAO levels and CVD risk factors.

AB - Scope: The impact of dietary protein types on the gut microbiome is scarcely studied. The aim of the present study is therefore to examine the effects of lean-seafood and non-seafood proteins on the gut microbiome composition and activity and elucidate potential associations to cardiovascular disease (CVD) risk factors. Methods: A crossover intervention study in which 20 healthy subjects consumed two diets that varied in protein source was conducted. 1H NMR spectroscopy and 16S rDNA sequencing analyses were applied to characterize fecal metabolites and gut microbiota composition, respectively. Results: A twofold increase in fecal trimethylamine excretion was observed after the lean-seafood diet period. Circulating TAG and the total to high-density lipoprotein (HDL) cholesterol ratio as well as circulating TMAO levels were each associated with specific gut bacteria. Following the non-seafood diet period, a decreased relative abundance of Clostridium cluster IV and a tendency toward an increased Firmicutes/Bacteroidetes ratio were found. Conclusions: Lean-seafood and non-seafood diets differentially modulate the gut microbiome composition and activity. Furthermore, the gut microbiota composition seems to affect circulating TMAO levels and CVD risk factors.

KW - 16S rDNA

KW - feces

KW - gut microbiota

KW - seafood protein

KW - TMAO

U2 - 10.1002/mnfr.201700976

DO - 10.1002/mnfr.201700976

M3 - Journal article

C2 - 29509315

AN - SCOPUS:85045835954

SN - 1613-4125

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JO - Molecular Nutrition & Food Research

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ER -

The Effect of Lean-Seafood and Non-Seafood Diets on Fecal Metabolites and Gut Microbiome: Results from a Randomized Crossover Intervention Study

Abstract

Keywords

UN SDGs

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