The effect of feeding on CO2 production and energy expenditure in ponies measured by indirect calorimetry and the 13C-bicarbonate technique

Rasmus Bovbjerg Jensen; T. D. Kyrstein; P Junghans; Anne-Helene Tauson

doi:10.1017/s1751731115001330

The effect of feeding on CO₂ production and energy expenditure in ponies measured by indirect calorimetry and the¹³C-bicarbonate technique

Rasmus Bovbjerg Jensen, T. D. Kyrstein, P Junghans, Anne-Helene Tauson

2 Citations (Scopus)

Abstract

Energy expenditure (EE) can be estimated based on respiratory gas exchange measurements, traditionally done in respiration chambers by indirect calorimetry (IC). However, the ¹³C-bicarbonate technique (¹³C-BT) might be an alternative minimal invasive method for estimation of CO2 production and EE in the field. In this study, four Shetland ponies were used to explore the effect of feeding on CO2 production and EE measured simultaneously by IC and ¹³C-BT. The ponies were individually housed in respiration chambers and received either a single oral or intravenous (IV) bolus dose of ¹³C-labelled sodium bicarbonate (NaH¹³CO3). The ponies were fed haylage 3 h before (T -3), simultaneously with (T 0) or 3 h after (T +3) administration of ¹³C-bicarbonate. The CO2 produced and O2 consumed by the ponies were measured for 6 h with both administration routes of ¹³C-bicarbonate at the three different feeding times. Feeding time affected the CO2 production (P<0.001) and O2 consumption (P<0.001), but not the respiratory quotient (RQ) measured by IC. The recovery factor (RF) of ¹³C in breath CO2 was affected by feeding time (P<0.01) and three different RF were used in the calculation of CO2 production measured by ¹³C-BT. An average RQ was used for the calculations of EE. There was no difference between IC and ¹³C-BT for estimation of CO2 production. An effect of feeding time (P<0.001) on the estimated EE was found, with higher EE when feed was offered (T 0 and T +3) compared with when no feed was available (T -3) during measurements. In conclusion, this study showed that feeding time affects the RF and measurements of CO2 production and EE. This should be considered when the ¹³C-BT is used in the field. IV administration of ¹³C-bicarbonate is recommended in future studies with horses to avoid complex ¹³C enrichment-time curves with maxima and shoulders as observed in several experiments with oral administration of ¹³C-bicarbonate.

Original language	English
Journal	Animal
Volume	9
Issue number	11
Pages (from-to)	1778-1785
Number of pages	8
ISSN	1751-7311
DOIs	https://doi.org/10.1017/s1751731115001330
Publication status	Published - 10 Jul 2015

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10.1017/s1751731115001330Licence: CC BY-NC-ND

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@article{56096c30fa134bd7b8e9528a6ef66234,

title = "The effect of feeding on CO2 production and energy expenditure in ponies measured by indirect calorimetry and the 13C-bicarbonate technique",

abstract = "Energy expenditure (EE) can be estimated based on respiratory gas exchange measurements, traditionally done in respiration chambers by indirect calorimetry (IC). However, the 13C-bicarbonate technique (13C-BT) might be an alternative minimal invasive method for estimation of CO2 production and EE in the field. In this study, four Shetland ponies were used to explore the effect of feeding on CO2 production and EE measured simultaneously by IC and 13C-BT. The ponies were individually housed in respiration chambers and received either a single oral or intravenous (IV) bolus dose of 13C-labelled sodium bicarbonate (NaH13CO3). The ponies were fed haylage 3 h before (T -3), simultaneously with (T 0) or 3 h after (T +3) administration of 13C-bicarbonate. The CO2 produced and O2 consumed by the ponies were measured for 6 h with both administration routes of 13C-bicarbonate at the three different feeding times. Feeding time affected the CO2 production (P<0.001) and O2 consumption (P<0.001), but not the respiratory quotient (RQ) measured by IC. The recovery factor (RF) of 13C in breath CO2 was affected by feeding time (P<0.01) and three different RF were used in the calculation of CO2 production measured by 13C-BT. An average RQ was used for the calculations of EE. There was no difference between IC and 13C-BT for estimation of CO2 production. An effect of feeding time (P<0.001) on the estimated EE was found, with higher EE when feed was offered (T 0 and T +3) compared with when no feed was available (T -3) during measurements. In conclusion, this study showed that feeding time affects the RF and measurements of CO2 production and EE. This should be considered when the 13C-BT is used in the field. IV administration of 13C-bicarbonate is recommended in future studies with horses to avoid complex 13C enrichment-time curves with maxima and shoulders as observed in several experiments with oral administration of 13C-bicarbonate.",

author = "Jensen, {Rasmus Bovbjerg} and Kyrstein, {T. D.} and P Junghans and Anne-Helene Tauson",

year = "2015",

month = jul,

day = "10",

doi = "10.1017/s1751731115001330",

language = "English",

volume = "9",

pages = "1778--1785",

journal = "Reproduction Nutrition Development",

issn = "1751-7311",

publisher = "Cambridge University Press",

number = "11",

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TY - JOUR

T1 - The effect of feeding on CO2 production and energy expenditure in ponies measured by indirect calorimetry and the 13C-bicarbonate technique

AU - Jensen, Rasmus Bovbjerg

AU - Kyrstein, T. D.

AU - Junghans, P

AU - Tauson, Anne-Helene

PY - 2015/7/10

Y1 - 2015/7/10

N2 - Energy expenditure (EE) can be estimated based on respiratory gas exchange measurements, traditionally done in respiration chambers by indirect calorimetry (IC). However, the 13C-bicarbonate technique (13C-BT) might be an alternative minimal invasive method for estimation of CO2 production and EE in the field. In this study, four Shetland ponies were used to explore the effect of feeding on CO2 production and EE measured simultaneously by IC and 13C-BT. The ponies were individually housed in respiration chambers and received either a single oral or intravenous (IV) bolus dose of 13C-labelled sodium bicarbonate (NaH13CO3). The ponies were fed haylage 3 h before (T -3), simultaneously with (T 0) or 3 h after (T +3) administration of 13C-bicarbonate. The CO2 produced and O2 consumed by the ponies were measured for 6 h with both administration routes of 13C-bicarbonate at the three different feeding times. Feeding time affected the CO2 production (P<0.001) and O2 consumption (P<0.001), but not the respiratory quotient (RQ) measured by IC. The recovery factor (RF) of 13C in breath CO2 was affected by feeding time (P<0.01) and three different RF were used in the calculation of CO2 production measured by 13C-BT. An average RQ was used for the calculations of EE. There was no difference between IC and 13C-BT for estimation of CO2 production. An effect of feeding time (P<0.001) on the estimated EE was found, with higher EE when feed was offered (T 0 and T +3) compared with when no feed was available (T -3) during measurements. In conclusion, this study showed that feeding time affects the RF and measurements of CO2 production and EE. This should be considered when the 13C-BT is used in the field. IV administration of 13C-bicarbonate is recommended in future studies with horses to avoid complex 13C enrichment-time curves with maxima and shoulders as observed in several experiments with oral administration of 13C-bicarbonate.

AB - Energy expenditure (EE) can be estimated based on respiratory gas exchange measurements, traditionally done in respiration chambers by indirect calorimetry (IC). However, the 13C-bicarbonate technique (13C-BT) might be an alternative minimal invasive method for estimation of CO2 production and EE in the field. In this study, four Shetland ponies were used to explore the effect of feeding on CO2 production and EE measured simultaneously by IC and 13C-BT. The ponies were individually housed in respiration chambers and received either a single oral or intravenous (IV) bolus dose of 13C-labelled sodium bicarbonate (NaH13CO3). The ponies were fed haylage 3 h before (T -3), simultaneously with (T 0) or 3 h after (T +3) administration of 13C-bicarbonate. The CO2 produced and O2 consumed by the ponies were measured for 6 h with both administration routes of 13C-bicarbonate at the three different feeding times. Feeding time affected the CO2 production (P<0.001) and O2 consumption (P<0.001), but not the respiratory quotient (RQ) measured by IC. The recovery factor (RF) of 13C in breath CO2 was affected by feeding time (P<0.01) and three different RF were used in the calculation of CO2 production measured by 13C-BT. An average RQ was used for the calculations of EE. There was no difference between IC and 13C-BT for estimation of CO2 production. An effect of feeding time (P<0.001) on the estimated EE was found, with higher EE when feed was offered (T 0 and T +3) compared with when no feed was available (T -3) during measurements. In conclusion, this study showed that feeding time affects the RF and measurements of CO2 production and EE. This should be considered when the 13C-BT is used in the field. IV administration of 13C-bicarbonate is recommended in future studies with horses to avoid complex 13C enrichment-time curves with maxima and shoulders as observed in several experiments with oral administration of 13C-bicarbonate.

U2 - 10.1017/s1751731115001330

DO - 10.1017/s1751731115001330

M3 - Journal article

C2 - 26477528

SN - 1751-7311

VL - 9

SP - 1778

EP - 1785

JO - Reproduction Nutrition Development

JF - Reproduction Nutrition Development

IS - 11

ER -

The effect of feeding on CO2 production and energy expenditure in ponies measured by indirect calorimetry and the 13C-bicarbonate technique

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The effect of feeding on CO₂ production and energy expenditure in ponies measured by indirect calorimetry and the¹³C-bicarbonate technique