TY - JOUR
T1 - The effect of dietary estimates calculated using food frequency questionnaires on micronuclei formation in European pregnant women
T2 - a NewGeneris study
AU - Vande Loock, Kim
AU - Botsivali, Maria
AU - Zangogianni, Marina
AU - Anderson, Diana
AU - Baumgartner, Adolph
AU - Fthenou, Eleni
AU - Chatzi, Leda
AU - Marcos, Ricard
AU - Agramunt, Sylvia
AU - Namork, Ellen
AU - Granum, Berit
AU - Knudsen, Lisbeth E.
AU - Nielssen, Jeanette K S
AU - Meltzer, Helle Margrete
AU - Haugen, Margaretha
AU - Kyrtopoulos, Soterios A
AU - Decordier, Ilse
AU - Plas, Gina
AU - Roelants, Mathieu
AU - Merlo, Franco
AU - Kleinjans, Jos
AU - Kogevinas, Manolis
AU - Kirsch-Volders, Micheline
N1 - © The Author 2014. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please e-mail: [email protected].
PY - 2014/11/1
Y1 - 2014/11/1
N2 - The use of biomarkers of early genetic effects, predictive for cancer, such as micronuclei (MN) in lymphocytes, may help to investigate the association between diet and cancer. We hypothesised that the presence of mutagens in the diet may increase MN formation. A 'pooled' standardised analysis was performed by applying the same experimental protocol for the cytokinesis block micronucleus assay in 625 young healthy women after delivery from five European study populations (Greece, Denmark, UK, Spain and Norway). We assessed MN frequencies in mono- and binucleated T-lymphocytes (MNMONO and MNBN) and the cytokinesis blocked proliferation index using a semi-automated image analysis system. Food frequency questionnaires (FFQs) were used to estimate intake of fatty acids and a broad range of immunotoxic and genotoxic/carcinogenic compounds through the diet. Pooled difference based on delivery type revealed higher MNMONO frequencies in caesarean than in vaginal delivery (P = 0.002). Statistical analysis showed a decrease in MNMONO frequencies with increasing calculated omega-6 PUFA concentrations and a decrease in MNBN frequencies with increasing calculated omega-3 PUFA concentrations. The expected toxic compounds estimated by FFQs were not associated with MN formation in mothers after delivery. In pregnant women, an omega-3 and -6 rich diet estimated by FFQ is associated with lower MN formation during pregnancy and delivery.
AB - The use of biomarkers of early genetic effects, predictive for cancer, such as micronuclei (MN) in lymphocytes, may help to investigate the association between diet and cancer. We hypothesised that the presence of mutagens in the diet may increase MN formation. A 'pooled' standardised analysis was performed by applying the same experimental protocol for the cytokinesis block micronucleus assay in 625 young healthy women after delivery from five European study populations (Greece, Denmark, UK, Spain and Norway). We assessed MN frequencies in mono- and binucleated T-lymphocytes (MNMONO and MNBN) and the cytokinesis blocked proliferation index using a semi-automated image analysis system. Food frequency questionnaires (FFQs) were used to estimate intake of fatty acids and a broad range of immunotoxic and genotoxic/carcinogenic compounds through the diet. Pooled difference based on delivery type revealed higher MNMONO frequencies in caesarean than in vaginal delivery (P = 0.002). Statistical analysis showed a decrease in MNMONO frequencies with increasing calculated omega-6 PUFA concentrations and a decrease in MNBN frequencies with increasing calculated omega-3 PUFA concentrations. The expected toxic compounds estimated by FFQs were not associated with MN formation in mothers after delivery. In pregnant women, an omega-3 and -6 rich diet estimated by FFQ is associated with lower MN formation during pregnancy and delivery.
U2 - 10.1093/mutage/geu052
DO - 10.1093/mutage/geu052
M3 - Journal article
C2 - 25296962
SN - 0267-8357
VL - 29
SP - 393
EP - 400
JO - Mutagenesis
JF - Mutagenesis
IS - 6
ER -
