The determination and analysis of site-specific rates of mitochondrial reactive oxygen species production

Casey L Quinlan; Irina V Perevoschikova; Renata L S Goncalves; Martin Hey-Mogensen; Martin D Brand

doi:10.1016/B978-0-12-405883-5.00012-0

The determination and analysis of site-specific rates of mitochondrial reactive oxygen species production

Casey L Quinlan, Irina V Perevoschikova, Renata L S Goncalves, Martin Hey-Mogensen, Martin D Brand

Torekov Group

51 Citations (Scopus)

Abstract

Mitochondrial reactive oxygen species (ROS) are widely implicated in physiological and pathological pathways. We propose that it is critical to understand the specific sites of mitochondrial ROS production and their mechanisms of action. Mitochondria possess at least eight distinct sites of ROS production in the electron transport chain and matrix compartment. In this chapter, we describe the nature of the mitochondrial ROS-producing machinery and the relative capacities of each site. We provide detailed methods for the measurement of H2O2 release and the conditions under which maximal rates from each site can be achieved in intact skeletal muscle mitochondria.

Original language	English
Title of host publication	The determination and analysis of site-specific rates of mitochondrial reactive oxygen species production
Number of pages	29
Volume	526
Publication date	2013
Pages	189-217
Chapter	12
DOIs	https://doi.org/10.1016/B978-0-12-405883-5.00012-0
Publication status	Published - 2013

Series	Methods in Enzymology
ISSN	0076-6879

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Quinlan, C. L., Perevoschikova, I. V., Goncalves, R. L. S., Hey-Mogensen, M., & Brand, M. D. (2013). The determination and analysis of site-specific rates of mitochondrial reactive oxygen species production. In The determination and analysis of site-specific rates of mitochondrial reactive oxygen species production (Vol. 526, pp. 189-217). Methods in Enzymology https://doi.org/10.1016/B978-0-12-405883-5.00012-0

The determination and analysis of site-specific rates of mitochondrial reactive oxygen species production. / Quinlan, Casey L; Perevoschikova, Irina V; Goncalves, Renata L S et al.

The determination and analysis of site-specific rates of mitochondrial reactive oxygen species production. Vol. 526 2013. p. 189-217 (Methods in Enzymology).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Quinlan, CL, Perevoschikova, IV, Goncalves, RLS, Hey-Mogensen, M & Brand, MD 2013, The determination and analysis of site-specific rates of mitochondrial reactive oxygen species production. in The determination and analysis of site-specific rates of mitochondrial reactive oxygen species production. vol. 526, Methods in Enzymology, pp. 189-217. https://doi.org/10.1016/B978-0-12-405883-5.00012-0

Quinlan CL, Perevoschikova IV, Goncalves RLS, Hey-Mogensen M, Brand MD. The determination and analysis of site-specific rates of mitochondrial reactive oxygen species production. In The determination and analysis of site-specific rates of mitochondrial reactive oxygen species production. Vol. 526. 2013. p. 189-217. (Methods in Enzymology). doi: 10.1016/B978-0-12-405883-5.00012-0

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BT - The determination and analysis of site-specific rates of mitochondrial reactive oxygen species production

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The determination and analysis of site-specific rates of mitochondrial reactive oxygen species production

Abstract

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