The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II

Hans Elmlund; Vera Baraznenok; Martin Lindahl; Camilla O Samuelsen; Philip J B Koeck; Steen Holmberg; Hans Hebert; Claes M Gustafsson

doi:10.1073/pnas.0607483103

The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II

Hans Elmlund, Vera Baraznenok, Martin Lindahl, Camilla O Samuelsen, Philip J B Koeck, Steen Holmberg, Hans Hebert, Claes M Gustafsson

Functional Genomics

141 Citations (Scopus)

Abstract

CDK8 (cyclin-dependent kinase 8), along with CycC, Med12, and Med13, form a repressive module (the Cdk8 module) that prevents RNA polymerase II (pol II) interactions with Mediator. Here, we report that the ability of the Cdk8 module to prevent pol II interactions is independent of the Cdk8-dependent kinase activity. We use electron microscopy and single-particle reconstruction to demonstrate that the Cdk8 module forms a distinct structural entity that binds to the head and middle region of Mediator, thereby sterically blocking interactions with pol II.

Original language	English
Journal	Proceedings of the National Academy of Science of the United States of America
Volume	103
Issue number	43
Pages (from-to)	15788-93
Number of pages	5
ISSN	0027-8424
DOIs	https://doi.org/10.1073/pnas.0607483103
Publication status	Published - 2006

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Elmlund, H., Baraznenok, V., Lindahl, M., Samuelsen, C. O., Koeck, P. J. B., Holmberg, S., Hebert, H., & Gustafsson, C. M. (2006). The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II. Proceedings of the National Academy of Science of the United States of America, 103(43), 15788-93. https://doi.org/10.1073/pnas.0607483103

@article{d43567a0cdb411dd9473000ea68e967b,

title = "The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II",

abstract = "CDK8 (cyclin-dependent kinase 8), along with CycC, Med12, and Med13, form a repressive module (the Cdk8 module) that prevents RNA polymerase II (pol II) interactions with Mediator. Here, we report that the ability of the Cdk8 module to prevent pol II interactions is independent of the Cdk8-dependent kinase activity. We use electron microscopy and single-particle reconstruction to demonstrate that the Cdk8 module forms a distinct structural entity that binds to the head and middle region of Mediator, thereby sterically blocking interactions with pol II.",

author = "Hans Elmlund and Vera Baraznenok and Martin Lindahl and Samuelsen, {Camilla O} and Koeck, {Philip J B} and Steen Holmberg and Hans Hebert and Gustafsson, {Claes M}",

note = "Keywords: Cyclin-Dependent Kinases; Holoenzymes; Microscopy, Electron; Models, Molecular; Protein Binding; Protein Structure, Quaternary; Protein Structure, Tertiary; RNA Polymerase II; Schizosaccharomyces; Schizosaccharomyces pombe Proteins",

year = "2006",

doi = "10.1073/pnas.0607483103",

language = "English",

volume = "103",

pages = "15788--93",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

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publisher = "The National Academy of Sciences of the United States of America",

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TY - JOUR

T1 - The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II

AU - Elmlund, Hans

AU - Baraznenok, Vera

AU - Lindahl, Martin

AU - Samuelsen, Camilla O

AU - Koeck, Philip J B

AU - Holmberg, Steen

AU - Hebert, Hans

AU - Gustafsson, Claes M

N1 - Keywords: Cyclin-Dependent Kinases; Holoenzymes; Microscopy, Electron; Models, Molecular; Protein Binding; Protein Structure, Quaternary; Protein Structure, Tertiary; RNA Polymerase II; Schizosaccharomyces; Schizosaccharomyces pombe Proteins

PY - 2006

Y1 - 2006

N2 - CDK8 (cyclin-dependent kinase 8), along with CycC, Med12, and Med13, form a repressive module (the Cdk8 module) that prevents RNA polymerase II (pol II) interactions with Mediator. Here, we report that the ability of the Cdk8 module to prevent pol II interactions is independent of the Cdk8-dependent kinase activity. We use electron microscopy and single-particle reconstruction to demonstrate that the Cdk8 module forms a distinct structural entity that binds to the head and middle region of Mediator, thereby sterically blocking interactions with pol II.

AB - CDK8 (cyclin-dependent kinase 8), along with CycC, Med12, and Med13, form a repressive module (the Cdk8 module) that prevents RNA polymerase II (pol II) interactions with Mediator. Here, we report that the ability of the Cdk8 module to prevent pol II interactions is independent of the Cdk8-dependent kinase activity. We use electron microscopy and single-particle reconstruction to demonstrate that the Cdk8 module forms a distinct structural entity that binds to the head and middle region of Mediator, thereby sterically blocking interactions with pol II.

U2 - 10.1073/pnas.0607483103

DO - 10.1073/pnas.0607483103

M3 - Journal article

C2 - 17043218

SN - 0027-8424

VL - 103

SP - 15788

EP - 15793

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 43

ER -

The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II

Abstract

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